Aeromonas phage phiARM81mr

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9KFC4|A0A1I9KFC4_9CAUD 23S rRNA-intervening sequence protein OS=Aeromonas phage phiARM81mr OX=1754209 GN=ARM81mr_p58 PE=4 SV=1
MM1 pKa = 7.63AGFVFLGNPQPTYY14 pKa = 8.75WTISMHH20 pKa = 6.09WSDD23 pKa = 4.21AVVNGITVTGVVTQVRR39 pKa = 11.84EE40 pKa = 4.14YY41 pKa = 10.94DD42 pKa = 3.41GSQALVTLNTGVSVVVPATHH62 pKa = 6.4EE63 pKa = 4.19PVPGDD68 pKa = 3.78AIVEE72 pKa = 4.45GEE74 pKa = 4.13LSLL77 pKa = 5.07

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9KFE6|A0A1I9KFE6_9CAUD Repressor OS=Aeromonas phage phiARM81mr OX=1754209 GN=ARM81mr_p07 PE=4 SV=1
MM1 pKa = 7.25GRR3 pKa = 11.84KK4 pKa = 8.82SDD6 pKa = 3.65RR7 pKa = 11.84PSRR10 pKa = 11.84AHH12 pKa = 6.2HH13 pKa = 6.49GDD15 pKa = 3.14RR16 pKa = 11.84GRR18 pKa = 11.84RR19 pKa = 11.84AAWLGVEE26 pKa = 4.5YY27 pKa = 10.9SHH29 pKa = 7.28GPRR32 pKa = 11.84PVCQVQEE39 pKa = 3.89QHH41 pKa = 5.53QGKK44 pKa = 7.0TVLVPRR50 pKa = 11.84PCAEE54 pKa = 3.8VLQPAKK60 pKa = 10.77

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

18389

40

3190

262.7

28.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.018 ± 0.478

1.055 ± 0.221

5.846 ± 0.249

6.874 ± 0.265

2.703 ± 0.154

7.466 ± 0.395

2.056 ± 0.236

4.394 ± 0.172

5.128 ± 0.312

8.315 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.806 ± 0.2

3.622 ± 0.169

4.427 ± 0.267

5.346 ± 0.354

6.738 ± 0.265

5.683 ± 0.305

5.607 ± 0.292

6.047 ± 0.246

1.485 ± 0.124

2.387 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski