Cryobacterium psychrophilum

Taxonomy:

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8KKD4|A0A4Y8KKD4_9MICO Uncharacterized protein OS=Cryobacterium psychrophilum OX=41988 GN=E3T53_15710 PE=4 SV=1
MM1 pKa = 7.4NNLGRR6 pKa = 11.84SFAGVALVTGLVATLAACSAAEE28 pKa = 4.08PTNVADD34 pKa = 5.34DD35 pKa = 3.96CTPDD39 pKa = 3.58YY40 pKa = 11.18VFSTIEE46 pKa = 4.09SGTLTVGLTEE56 pKa = 4.75IPPFSYY62 pKa = 10.55TDD64 pKa = 3.29NGDD67 pKa = 3.39ATGVDD72 pKa = 3.68VDD74 pKa = 4.23IVSAFADD81 pKa = 4.23ANCLTVDD88 pKa = 4.0YY89 pKa = 11.47VPVTYY94 pKa = 10.39SAAVPAVEE102 pKa = 5.01SKK104 pKa = 10.89RR105 pKa = 11.84IDD107 pKa = 3.47LTIGDD112 pKa = 4.57WYY114 pKa = 9.34RR115 pKa = 11.84TAARR119 pKa = 11.84SEE121 pKa = 4.17IVNFTAPNYY130 pKa = 10.06LDD132 pKa = 3.68EE133 pKa = 5.1FSVISEE139 pKa = 4.21TGITTVEE146 pKa = 4.03GLVGQKK152 pKa = 10.11VGTVEE157 pKa = 4.29GYY159 pKa = 10.74LWVDD163 pKa = 3.79DD164 pKa = 5.11LRR166 pKa = 11.84TLLDD170 pKa = 3.67SDD172 pKa = 3.94LQVYY176 pKa = 9.14PSSVEE181 pKa = 3.78LKK183 pKa = 10.24QDD185 pKa = 3.19IEE187 pKa = 4.08AGRR190 pKa = 11.84INVGVDD196 pKa = 3.04AFGTSLYY203 pKa = 11.08NFGDD207 pKa = 3.1TDD209 pKa = 4.0YY210 pKa = 11.35QVTTVAPDD218 pKa = 3.37EE219 pKa = 4.42RR220 pKa = 11.84VLATVEE226 pKa = 3.85AAQTGFPYY234 pKa = 10.0TQDD237 pKa = 3.39NTDD240 pKa = 3.23MGIALDD246 pKa = 3.71ATIEE250 pKa = 4.16DD251 pKa = 4.14LHH253 pKa = 8.72ASGGMVDD260 pKa = 3.09ILEE263 pKa = 4.29AHH265 pKa = 7.22GLPASAEE272 pKa = 4.31KK273 pKa = 10.74VGEE276 pKa = 3.96PRR278 pKa = 11.84LIEE281 pKa = 3.98

Molecular weight: 29.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8KMU4|A0A4Y8KMU4_9MICO Transglutaminase domain-containing protein OS=Cryobacterium psychrophilum OX=41988 GN=E3T53_10940 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3267

0

3267

1042130

32

1981

319.0

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.782 ± 0.06

0.578 ± 0.011

5.74 ± 0.038

5.298 ± 0.038

3.251 ± 0.028

8.661 ± 0.033

2.087 ± 0.017

4.873 ± 0.034

2.272 ± 0.029

10.463 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.93 ± 0.017

2.433 ± 0.025

5.186 ± 0.031

2.887 ± 0.02

6.857 ± 0.04

6.119 ± 0.029

6.407 ± 0.034

8.757 ± 0.038

1.414 ± 0.018

2.004 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski