Oscillibacter valericigenes (strain DSM 18026 / NBRC 101213 / Sjm18-20)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Oscillibacter; Oscillibacter valericigenes

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4KRV1|G4KRV1_OSCVS Putative transposase orfB for insertion sequence element OS=Oscillibacter valericigenes (strain DSM 18026 / NBRC 101213 / Sjm18-20) OX=693746 GN=OBV_27690 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.26RR3 pKa = 11.84AFLAALAAMLLLSGCGKK20 pKa = 9.75QAEE23 pKa = 4.64EE24 pKa = 4.13PSGLYY29 pKa = 10.24YY30 pKa = 10.16EE31 pKa = 5.08ASGIAPDD38 pKa = 4.12TVLLTVDD45 pKa = 3.36GRR47 pKa = 11.84EE48 pKa = 4.19VPAARR53 pKa = 11.84YY54 pKa = 7.94FYY56 pKa = 10.46WLTADD61 pKa = 3.89CDD63 pKa = 4.04YY64 pKa = 11.18LAEE67 pKa = 4.16QSGGEE72 pKa = 4.03PDD74 pKa = 3.12WTGDD78 pKa = 4.06AGGQTLDD85 pKa = 4.13EE86 pKa = 4.32YY87 pKa = 11.73VKK89 pKa = 11.01AQALSAAVFYY99 pKa = 9.3ATLEE103 pKa = 4.21SLAEE107 pKa = 4.05SHH109 pKa = 7.5DD110 pKa = 4.25CVLTAGDD117 pKa = 4.5LAAMDD122 pKa = 5.19KK123 pKa = 11.0DD124 pKa = 3.69WADD127 pKa = 3.28QAAAAGGEE135 pKa = 4.04DD136 pKa = 4.0AYY138 pKa = 11.48LAVLAQKK145 pKa = 11.01GLDD148 pKa = 3.35QAGAQLFSADD158 pKa = 3.56YY159 pKa = 10.67YY160 pKa = 11.18LYY162 pKa = 10.17EE163 pKa = 4.05QLRR166 pKa = 11.84EE167 pKa = 4.01LSQTEE172 pKa = 4.36GNDD175 pKa = 3.6LAPKK179 pKa = 10.3SGDD182 pKa = 3.14VTAYY186 pKa = 9.99AQAAGLYY193 pKa = 8.73TADD196 pKa = 3.8TLLFSDD202 pKa = 4.43GDD204 pKa = 3.98LAAVALSEE212 pKa = 4.77LEE214 pKa = 4.37KK215 pKa = 11.33DD216 pKa = 3.68PDD218 pKa = 4.31LDD220 pKa = 4.18PATLKK225 pKa = 10.99DD226 pKa = 3.36GTYY229 pKa = 10.9ASVTAAPGDD238 pKa = 3.74GTLPEE243 pKa = 4.29AAEE246 pKa = 4.22SALKK250 pKa = 10.64GLQTDD255 pKa = 3.22QWSQPVEE262 pKa = 4.35TEE264 pKa = 3.53AGVYY268 pKa = 10.07LLRR271 pKa = 11.84PAALDD276 pKa = 3.31EE277 pKa = 4.85DD278 pKa = 4.37AAGDD282 pKa = 3.51LWFDD286 pKa = 4.63GRR288 pKa = 11.84LQDD291 pKa = 4.63LAADD295 pKa = 4.06APVTLAEE302 pKa = 4.5EE303 pKa = 4.52YY304 pKa = 11.22GSFTAASFYY313 pKa = 10.92KK314 pKa = 9.91GLCAARR320 pKa = 11.84VGGKK324 pKa = 9.43

Molecular weight: 33.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4KZ25|G4KZ25_OSCVS Stage 0 sporulation protein A homolog OS=Oscillibacter valericigenes (strain DSM 18026 / NBRC 101213 / Sjm18-20) OX=693746 GN=OBV_40180 PE=4 SV=1
MM1 pKa = 7.86AGRR4 pKa = 11.84NTQLPLARR12 pKa = 11.84MRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84TMSPVLRR23 pKa = 11.84SRR25 pKa = 11.84LTGLLSRR32 pKa = 11.84RR33 pKa = 11.84NTASHH38 pKa = 6.65RR39 pKa = 11.84CIFSCFRR46 pKa = 11.84RR47 pKa = 11.84GTRR50 pKa = 11.84ALPRR54 pKa = 11.84LPFPAWPGAVSAISSPQSKK73 pKa = 9.82IKK75 pKa = 10.82AGAA78 pKa = 3.24

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4593

0

4593

1245691

31

3221

271.2

29.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.254 ± 0.048

1.719 ± 0.018

5.503 ± 0.035

6.408 ± 0.043

3.94 ± 0.027

7.643 ± 0.038

1.789 ± 0.016

5.939 ± 0.04

5.379 ± 0.035

9.608 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.022

3.737 ± 0.026

3.943 ± 0.027

3.418 ± 0.025

5.447 ± 0.041

6.18 ± 0.042

5.763 ± 0.044

6.933 ± 0.034

1.015 ± 0.013

3.54 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski