Planctopirus limnophila (strain ATCC 43296 / DSM 3776 / IFAM 1008 / 290) (Planctomyces limnophilus)

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Planctopirus; Planctopirus limnophila

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5SNE3|D5SNE3_PLAL2 AAA ATPase OS=Planctopirus limnophila (strain ATCC 43296 / DSM 3776 / IFAM 1008 / 290) OX=521674 GN=Plim_2231 PE=4 SV=1
MM1 pKa = 6.97EE2 pKa = 4.21TTRR5 pKa = 11.84EE6 pKa = 3.92LTAEE10 pKa = 4.12EE11 pKa = 4.52VGNLANQLQNAYY23 pKa = 10.76DD24 pKa = 4.43SIQDD28 pKa = 3.54SYY30 pKa = 11.76EE31 pKa = 3.93YY32 pKa = 10.4PLDD35 pKa = 4.31DD36 pKa = 6.26LIDD39 pKa = 3.95YY40 pKa = 7.55LQQSQSEE47 pKa = 4.52PIHH50 pKa = 5.74EE51 pKa = 4.37VEE53 pKa = 4.62EE54 pKa = 4.55SDD56 pKa = 4.48GVAARR61 pKa = 11.84YY62 pKa = 8.33FLYY65 pKa = 10.41FSSPPEE71 pKa = 3.66TWEE74 pKa = 3.9ALCGRR79 pKa = 11.84EE80 pKa = 4.81GIYY83 pKa = 10.01TVDD86 pKa = 3.86AKK88 pKa = 9.11TLRR91 pKa = 11.84ANNFDD96 pKa = 3.36ISVMNN101 pKa = 4.52

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5SMD4|D5SMD4_PLAL2 Chromosomal replication initiator protein DnaA OS=Planctopirus limnophila (strain ATCC 43296 / DSM 3776 / IFAM 1008 / 290) OX=521674 GN=dnaA PE=3 SV=1
MM1 pKa = 6.97PRR3 pKa = 11.84KK4 pKa = 10.05RR5 pKa = 11.84IPLEE9 pKa = 3.74PRR11 pKa = 11.84ACEE14 pKa = 4.01YY15 pKa = 10.86CGTVYY20 pKa = 10.28MPDD23 pKa = 3.06RR24 pKa = 11.84VTARR28 pKa = 11.84WCQSGCYY35 pKa = 9.52ARR37 pKa = 11.84WLIEE41 pKa = 3.67TRR43 pKa = 11.84KK44 pKa = 10.24AEE46 pKa = 3.85RR47 pKa = 11.84AARR50 pKa = 11.84RR51 pKa = 11.84IVYY54 pKa = 9.94EE55 pKa = 4.29MVCPICDD62 pKa = 3.01IKK64 pKa = 11.17FQTSHH69 pKa = 6.49HH70 pKa = 6.14AKK72 pKa = 9.78KK73 pKa = 10.33FCSEE77 pKa = 3.67KK78 pKa = 10.57CQARR82 pKa = 11.84DD83 pKa = 3.01SSQRR87 pKa = 11.84QIQRR91 pKa = 11.84LKK93 pKa = 10.21IDD95 pKa = 3.3RR96 pKa = 11.84EE97 pKa = 3.8TARR100 pKa = 11.84RR101 pKa = 11.84YY102 pKa = 9.61RR103 pKa = 11.84RR104 pKa = 11.84AHH106 pKa = 5.94IEE108 pKa = 3.97RR109 pKa = 11.84LRR111 pKa = 11.84STPEE115 pKa = 3.1GRR117 pKa = 11.84AKK119 pKa = 10.11IKK121 pKa = 10.23RR122 pKa = 11.84WNDD125 pKa = 2.99QKK127 pKa = 11.14NARR130 pKa = 11.84VSAEE134 pKa = 3.47RR135 pKa = 11.84RR136 pKa = 11.84QRR138 pKa = 11.84RR139 pKa = 11.84LDD141 pKa = 3.54RR142 pKa = 11.84DD143 pKa = 3.31KK144 pKa = 11.39EE145 pKa = 4.1

Molecular weight: 17.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4258

0

4258

1533192

30

7238

360.1

39.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.632 ± 0.042

1.126 ± 0.017

5.091 ± 0.03

6.239 ± 0.035

3.701 ± 0.021

7.409 ± 0.047

2.223 ± 0.024

5.23 ± 0.024

3.9 ± 0.036

10.423 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.018

3.109 ± 0.033

5.625 ± 0.045

4.497 ± 0.026

6.335 ± 0.037

6.789 ± 0.032

5.708 ± 0.051

6.849 ± 0.028

1.627 ± 0.019

2.28 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski