Amino acid dipepetide frequency for Thermoclostridium stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754) (Clostridium stercorarium)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.473AlaAla: 5.473 ± 0.102	0.884AlaCys: 0.884 ± 0.034	3.55AlaAsp: 3.55 ± 0.065	5.232AlaGlu: 5.232 ± 0.096	2.917AlaPhe: 2.917 ± 0.066	5.462AlaGly: 5.462 ± 0.089	1.006AlaHis: 1.006 ± 0.032	4.913AlaIle: 4.913 ± 0.076	4.239AlaLys: 4.239 ± 0.084	6.416AlaLeu: 6.416 ± 0.091	1.812AlaMet: 1.812 ± 0.051	2.41AlaAsn: 2.41 ± 0.052	1.816AlaPro: 1.816 ± 0.053	1.779AlaGln: 1.779 ± 0.039	3.173AlaArg: 3.173 ± 0.06	3.363AlaSer: 3.363 ± 0.074	2.89AlaThr: 2.89 ± 0.063	6.095AlaVal: 6.095 ± 0.096	0.557AlaTrp: 0.557 ± 0.029	2.528AlaTyr: 2.528 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.752CysAla: 0.752 ± 0.031	0.202CysCys: 0.202 ± 0.015	0.662CysAsp: 0.662 ± 0.03	0.766CysGlu: 0.766 ± 0.026	0.603CysPhe: 0.603 ± 0.026	1.111CysGly: 1.111 ± 0.04	0.245CysHis: 0.245 ± 0.019	1.017CysIle: 1.017 ± 0.033	0.616CysLys: 0.616 ± 0.028	1.023CysLeu: 1.023 ± 0.038	0.285CysMet: 0.285 ± 0.017	0.529CysAsn: 0.529 ± 0.025	0.542CysPro: 0.542 ± 0.028	0.209CysGln: 0.209 ± 0.015	0.732CysArg: 0.732 ± 0.033	0.749CysSer: 0.749 ± 0.032	0.541CysThr: 0.541 ± 0.025	0.804CysVal: 0.804 ± 0.034	0.099CysTrp: 0.099 ± 0.011	0.425CysTyr: 0.425 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.258AspAla: 3.258 ± 0.064	0.597AspCys: 0.597 ± 0.026	2.646AspAsp: 2.646 ± 0.062	4.717AspGlu: 4.717 ± 0.08	2.684AspPhe: 2.684 ± 0.062	4.019AspGly: 4.019 ± 0.077	0.595AspHis: 0.595 ± 0.027	5.271AspIle: 5.271 ± 0.076	3.525AspLys: 3.525 ± 0.067	4.349AspLeu: 4.349 ± 0.074	1.515AspMet: 1.515 ± 0.045	2.437AspAsn: 2.437 ± 0.058	2.045AspPro: 2.045 ± 0.049	0.949AspGln: 0.949 ± 0.031	2.832AspArg: 2.832 ± 0.061	2.611AspSer: 2.611 ± 0.061	2.621AspThr: 2.621 ± 0.055	3.663AspVal: 3.663 ± 0.061	0.585AspTrp: 0.585 ± 0.029	2.525AspTyr: 2.525 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	4.868GluAla: 4.868 ± 0.083	0.694GluCys: 0.694 ± 0.03	3.337GluAsp: 3.337 ± 0.063	5.943GluGlu: 5.943 ± 0.095	2.889GluPhe: 2.889 ± 0.066	4.139GluGly: 4.139 ± 0.07	1.153GluHis: 1.153 ± 0.04	6.88GluIle: 6.88 ± 0.106	7.197GluLys: 7.197 ± 0.094	6.748GluLeu: 6.748 ± 0.114	2.205GluMet: 2.205 ± 0.048	4.834GluAsn: 4.834 ± 0.077	2.078GluPro: 2.078 ± 0.052	2.198GluGln: 2.198 ± 0.072	3.698GluArg: 3.698 ± 0.078	3.106GluSer: 3.106 ± 0.065	3.725GluThr: 3.725 ± 0.057	4.237GluVal: 4.237 ± 0.071	0.752GluTrp: 0.752 ± 0.032	3.27GluTyr: 3.27 ± 0.071	0.0GluXaa: 0.0 ± 0.0
Phe	2.819PheAla: 2.819 ± 0.054	0.645PheCys: 0.645 ± 0.026	2.664PheAsp: 2.664 ± 0.056	2.935PheGlu: 2.935 ± 0.069	2.172PhePhe: 2.172 ± 0.063	3.12PheGly: 3.12 ± 0.071	0.686PheHis: 0.686 ± 0.029	3.755PheIle: 3.755 ± 0.069	2.467PheLys: 2.467 ± 0.049	4.169PheLeu: 4.169 ± 0.083	1.118PheMet: 1.118 ± 0.041	2.072PheAsn: 2.072 ± 0.049	1.579PhePro: 1.579 ± 0.047	1.009PheGln: 1.009 ± 0.037	2.17PheArg: 2.17 ± 0.051	3.3PheSer: 3.3 ± 0.066	2.549PheThr: 2.549 ± 0.051	2.983PheVal: 2.983 ± 0.065	0.465PheTrp: 0.465 ± 0.021	1.888PheTyr: 1.888 ± 0.047	0.001PheXaa: 0.001 ± 0.001
Gly	4.079GlyAla: 4.079 ± 0.078	1.002GlyCys: 1.002 ± 0.04	3.372GlyAsp: 3.372 ± 0.071	4.612GlyGlu: 4.612 ± 0.065	3.416GlyPhe: 3.416 ± 0.058	4.361GlyGly: 4.361 ± 0.09	1.131GlyHis: 1.131 ± 0.034	6.867GlyIle: 6.867 ± 0.083	5.462GlyLys: 5.462 ± 0.084	5.954GlyLeu: 5.954 ± 0.088	2.148GlyMet: 2.148 ± 0.053	3.55GlyAsn: 3.55 ± 0.066	1.562GlyPro: 1.562 ± 0.05	1.779GlyGln: 1.779 ± 0.046	3.358GlyArg: 3.358 ± 0.071	3.648GlySer: 3.648 ± 0.064	3.886GlyThr: 3.886 ± 0.082	4.695GlyVal: 4.695 ± 0.084	0.659GlyTrp: 0.659 ± 0.032	3.195GlyTyr: 3.195 ± 0.063	0.001GlyXaa: 0.001 ± 0.001
His	0.925HisAla: 0.925 ± 0.036	0.22HisCys: 0.22 ± 0.017	0.785HisAsp: 0.785 ± 0.035	1.092HisGlu: 1.092 ± 0.041	0.76HisPhe: 0.76 ± 0.033	1.235HisGly: 1.235 ± 0.039	0.303HisHis: 0.303 ± 0.019	1.312HisIle: 1.312 ± 0.047	0.85HisLys: 0.85 ± 0.032	1.297HisLeu: 1.297 ± 0.042	0.433HisMet: 0.433 ± 0.021	0.769HisAsn: 0.769 ± 0.028	0.841HisPro: 0.841 ± 0.031	0.387HisGln: 0.387 ± 0.022	0.833HisArg: 0.833 ± 0.031	0.84HisSer: 0.84 ± 0.036	0.832HisThr: 0.832 ± 0.035	1.058HisVal: 1.058 ± 0.038	0.179HisTrp: 0.179 ± 0.016	0.675HisTyr: 0.675 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.657IleAla: 5.657 ± 0.086	1.079IleCys: 1.079 ± 0.041	4.633IleAsp: 4.633 ± 0.078	5.836IleGlu: 5.836 ± 0.085	3.489IlePhe: 3.489 ± 0.082	5.032IleGly: 5.032 ± 0.075	1.29IleHis: 1.29 ± 0.041	6.988IleIle: 6.988 ± 0.114	5.439IleLys: 5.439 ± 0.095	7.749IleLeu: 7.749 ± 0.123	2.138IleMet: 2.138 ± 0.05	4.433IleAsn: 4.433 ± 0.078	3.974IlePro: 3.974 ± 0.067	2.183IleGln: 2.183 ± 0.052	4.359IleArg: 4.359 ± 0.071	5.676IleSer: 5.676 ± 0.085	4.829IleThr: 4.829 ± 0.084	5.572IleVal: 5.572 ± 0.086	0.655IleTrp: 0.655 ± 0.026	3.224IleTyr: 3.224 ± 0.072	0.0IleXaa: 0.0 ± 0.0
Lys	4.879LysAla: 4.879 ± 0.086	0.688LysCys: 0.688 ± 0.033	3.713LysAsp: 3.713 ± 0.082	5.64LysGlu: 5.64 ± 0.093	2.399LysPhe: 2.399 ± 0.053	4.481LysGly: 4.481 ± 0.068	1.098LysHis: 1.098 ± 0.038	6.039LysIle: 6.039 ± 0.086	5.994LysLys: 5.994 ± 0.101	5.941LysLeu: 5.941 ± 0.087	1.92LysMet: 1.92 ± 0.048	4.348LysAsn: 4.348 ± 0.071	2.454LysPro: 2.454 ± 0.06	1.969LysGln: 1.969 ± 0.05	3.381LysArg: 3.381 ± 0.067	3.646LysSer: 3.646 ± 0.077	3.861LysThr: 3.861 ± 0.078	4.671LysVal: 4.671 ± 0.087	0.721LysTrp: 0.721 ± 0.032	3.215LysTyr: 3.215 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	6.043LeuAla: 6.043 ± 0.085	1.135LeuCys: 1.135 ± 0.039	4.741LeuAsp: 4.741 ± 0.072	5.883LeuGlu: 5.883 ± 0.09	4.088LeuPhe: 4.088 ± 0.078	5.643LeuGly: 5.643 ± 0.096	1.269LeuHis: 1.269 ± 0.042	7.043LeuIle: 7.043 ± 0.107	7.207LeuLys: 7.207 ± 0.094	8.297LeuLeu: 8.297 ± 0.131	2.434LeuMet: 2.434 ± 0.059	4.674LeuAsn: 4.674 ± 0.081	3.615LeuPro: 3.615 ± 0.072	2.258LeuGln: 2.258 ± 0.05	4.123LeuArg: 4.123 ± 0.067	6.311LeuSer: 6.311 ± 0.096	4.915LeuThr: 4.915 ± 0.074	5.88LeuVal: 5.88 ± 0.095	0.783LeuTrp: 0.783 ± 0.029	3.75LeuTyr: 3.75 ± 0.073	0.001LeuXaa: 0.001 ± 0.001
Met	2.089MetAla: 2.089 ± 0.043	0.241MetCys: 0.241 ± 0.016	1.63MetAsp: 1.63 ± 0.047	2.033MetGlu: 2.033 ± 0.051	1.017MetPhe: 1.017 ± 0.039	1.854MetGly: 1.854 ± 0.05	0.484MetHis: 0.484 ± 0.022	1.926MetIle: 1.926 ± 0.046	2.215MetLys: 2.215 ± 0.053	2.589MetLeu: 2.589 ± 0.058	0.651MetMet: 0.651 ± 0.029	1.363MetAsn: 1.363 ± 0.04	1.163MetPro: 1.163 ± 0.039	0.853MetGln: 0.853 ± 0.029	1.191MetArg: 1.191 ± 0.037	1.583MetSer: 1.583 ± 0.042	1.34MetThr: 1.34 ± 0.041	1.878MetVal: 1.878 ± 0.055	0.189MetTrp: 0.189 ± 0.014	0.829MetTyr: 0.829 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.244AsnAla: 3.244 ± 0.069	0.588AsnCys: 0.588 ± 0.028	2.462AsnAsp: 2.462 ± 0.057	3.537AsnGlu: 3.537 ± 0.064	2.11AsnPhe: 2.11 ± 0.052	3.812AsnGly: 3.812 ± 0.063	0.734AsnHis: 0.734 ± 0.03	4.685AsnIle: 4.685 ± 0.092	2.992AsnLys: 2.992 ± 0.066	4.319AsnLeu: 4.319 ± 0.067	1.432AsnMet: 1.432 ± 0.039	2.585AsnAsn: 2.585 ± 0.065	2.458AsnPro: 2.458 ± 0.058	1.248AsnGln: 1.248 ± 0.043	2.714AsnArg: 2.714 ± 0.057	2.884AsnSer: 2.884 ± 0.06	2.678AsnThr: 2.678 ± 0.062	3.406AsnVal: 3.406 ± 0.071	0.472AsnTrp: 0.472 ± 0.025	2.004AsnTyr: 2.004 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	2.513ProAla: 2.513 ± 0.054	0.416ProCys: 0.416 ± 0.023	2.733ProAsp: 2.733 ± 0.063	3.77ProGlu: 3.77 ± 0.066	1.759ProPhe: 1.759 ± 0.044	2.796ProGly: 2.796 ± 0.057	0.655ProHis: 0.655 ± 0.028	2.055ProIle: 2.055 ± 0.051	1.973ProLys: 1.973 ± 0.049	2.932ProLeu: 2.932 ± 0.067	0.82ProMet: 0.82 ± 0.031	1.415ProAsn: 1.415 ± 0.042	1.078ProPro: 1.078 ± 0.04	0.916ProGln: 0.916 ± 0.033	1.241ProArg: 1.241 ± 0.04	2.003ProSer: 2.003 ± 0.05	1.645ProThr: 1.645 ± 0.056	3.813ProVal: 3.813 ± 0.072	0.381ProTrp: 0.381 ± 0.022	1.638ProTyr: 1.638 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	1.846GlnAla: 1.846 ± 0.063	0.239GlnCys: 0.239 ± 0.017	1.145GlnAsp: 1.145 ± 0.037	1.787GlnGlu: 1.787 ± 0.048	0.988GlnPhe: 0.988 ± 0.034	1.465GlnGly: 1.465 ± 0.049	0.381GlnHis: 0.381 ± 0.022	2.17GlnIle: 2.17 ± 0.049	2.298GlnLys: 2.298 ± 0.06	2.429GlnLeu: 2.429 ± 0.057	0.829GlnMet: 0.829 ± 0.032	1.494GlnAsn: 1.494 ± 0.044	0.832GlnPro: 0.832 ± 0.03	0.864GlnGln: 0.864 ± 0.041	1.347GlnArg: 1.347 ± 0.042	1.271GlnSer: 1.271 ± 0.036	1.23GlnThr: 1.23 ± 0.037	1.749GlnVal: 1.749 ± 0.054	0.318GlnTrp: 0.318 ± 0.017	1.084GlnTyr: 1.084 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.708ArgAla: 2.708 ± 0.049	0.479ArgCys: 0.479 ± 0.025	2.548ArgAsp: 2.548 ± 0.064	4.137ArgGlu: 4.137 ± 0.081	2.203ArgPhe: 2.203 ± 0.047	2.582ArgGly: 2.582 ± 0.054	0.871ArgHis: 0.871 ± 0.037	4.62ArgIle: 4.62 ± 0.08	4.185ArgLys: 4.185 ± 0.077	4.523ArgLeu: 4.523 ± 0.074	1.422ArgMet: 1.422 ± 0.045	2.793ArgAsn: 2.793 ± 0.056	1.513ArgPro: 1.513 ± 0.043	1.491ArgGln: 1.491 ± 0.042	2.363ArgArg: 2.363 ± 0.055	2.152ArgSer: 2.152 ± 0.051	2.288ArgThr: 2.288 ± 0.059	3.168ArgVal: 3.168 ± 0.066	0.381ArgTrp: 0.381 ± 0.021	2.156ArgTyr: 2.156 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	3.829SerAla: 3.829 ± 0.075	0.603SerCys: 0.603 ± 0.03	3.277SerAsp: 3.277 ± 0.063	4.206SerGlu: 4.206 ± 0.068	2.83SerPhe: 2.83 ± 0.062	5.04SerGly: 5.04 ± 0.097	0.924SerHis: 0.924 ± 0.033	4.446SerIle: 4.446 ± 0.085	3.345SerLys: 3.345 ± 0.063	5.17SerLeu: 5.17 ± 0.085	1.515SerMet: 1.515 ± 0.042	2.316SerAsn: 2.316 ± 0.057	2.065SerPro: 2.065 ± 0.053	1.486SerGln: 1.486 ± 0.043	2.824SerArg: 2.824 ± 0.059	3.307SerSer: 3.307 ± 0.069	2.535SerThr: 2.535 ± 0.057	4.554SerVal: 4.554 ± 0.09	0.543SerTrp: 0.543 ± 0.025	2.25SerTyr: 2.25 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	3.869ThrAla: 3.869 ± 0.07	0.542ThrCys: 0.542 ± 0.023	3.004ThrAsp: 3.004 ± 0.058	3.527ThrGlu: 3.527 ± 0.059	2.14ThrPhe: 2.14 ± 0.056	5.098ThrGly: 5.098 ± 0.087	0.802ThrHis: 0.802 ± 0.035	3.788ThrIle: 3.788 ± 0.071	2.652ThrLys: 2.652 ± 0.055	4.577ThrLeu: 4.577 ± 0.075	1.193ThrMet: 1.193 ± 0.036	2.014ThrAsn: 2.014 ± 0.047	2.316ThrPro: 2.316 ± 0.068	1.147ThrGln: 1.147 ± 0.039	2.149ThrArg: 2.149 ± 0.053	2.65ThrSer: 2.65 ± 0.051	2.459ThrThr: 2.459 ± 0.058	5.033ThrVal: 5.033 ± 0.092	0.45ThrTrp: 0.45 ± 0.023	1.903ThrTyr: 1.903 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	4.552ValAla: 4.552 ± 0.094	0.989ValCys: 0.989 ± 0.04	3.656ValAsp: 3.656 ± 0.067	4.817ValGlu: 4.817 ± 0.08	3.529ValPhe: 3.529 ± 0.067	3.928ValGly: 3.928 ± 0.075	1.052ValHis: 1.052 ± 0.035	6.302ValIle: 6.302 ± 0.1	5.184ValLys: 5.184 ± 0.074	6.977ValLeu: 6.977 ± 0.103	1.958ValMet: 1.958 ± 0.042	3.551ValAsn: 3.551 ± 0.068	2.673ValPro: 2.673 ± 0.052	1.617ValGln: 1.617 ± 0.048	3.338ValArg: 3.338 ± 0.062	4.903ValSer: 4.903 ± 0.074	3.854ValThr: 3.854 ± 0.079	5.052ValVal: 5.052 ± 0.09	0.62ValTrp: 0.62 ± 0.029	3.087ValTyr: 3.087 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.621TrpAla: 0.621 ± 0.028	0.123TrpCys: 0.123 ± 0.011	0.542TrpAsp: 0.542 ± 0.029	0.635TrpGlu: 0.635 ± 0.031	0.465TrpPhe: 0.465 ± 0.024	0.586TrpGly: 0.586 ± 0.023	0.223TrpHis: 0.223 ± 0.016	0.694TrpIle: 0.694 ± 0.03	0.644TrpLys: 0.644 ± 0.027	0.894TrpLeu: 0.894 ± 0.034	0.276TrpMet: 0.276 ± 0.021	0.574TrpAsn: 0.574 ± 0.029	0.285TrpPro: 0.285 ± 0.019	0.326TrpGln: 0.326 ± 0.02	0.359TrpArg: 0.359 ± 0.016	0.493TrpSer: 0.493 ± 0.026	0.43TrpThr: 0.43 ± 0.023	0.631TrpVal: 0.631 ± 0.026	0.132TrpTrp: 0.132 ± 0.012	0.421TrpTyr: 0.421 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.493TyrAla: 2.493 ± 0.052	0.548TyrCys: 0.548 ± 0.025	2.342TyrAsp: 2.342 ± 0.056	2.846TyrGlu: 2.846 ± 0.057	2.108TyrPhe: 2.108 ± 0.042	2.973TyrGly: 2.973 ± 0.062	0.724TyrHis: 0.724 ± 0.035	3.462TyrIle: 3.462 ± 0.071	2.575TyrLys: 2.575 ± 0.059	3.744TyrLeu: 3.744 ± 0.068	1.012TyrMet: 1.012 ± 0.037	2.202TyrAsn: 2.202 ± 0.053	1.66TyrPro: 1.66 ± 0.042	1.098TyrGln: 1.098 ± 0.039	2.396TyrArg: 2.396 ± 0.057	2.572TyrSer: 2.572 ± 0.061	2.204TyrThr: 2.204 ± 0.057	2.697TyrVal: 2.697 ± 0.055	0.411TyrTrp: 0.411 ± 0.023	2.042TyrTyr: 2.042 ± 0.058	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 2669 proteins (856082 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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