Austrofundulus limnaeus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata; Acanthomorphata;

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I4ALM3|A0A2I4ALM3_9TELE 15-hydroxyprostaglandin dehydrogenase [NAD(+)]-like OS=Austrofundulus limnaeus OX=52670 GN=LOC106512286 PE=3 SV=1
MM1 pKa = 7.54FSHH4 pKa = 7.11SDD6 pKa = 3.39DD7 pKa = 3.79SDD9 pKa = 4.3SFTHH13 pKa = 7.24PDD15 pKa = 3.37DD16 pKa = 4.95SDD18 pKa = 4.18SFTHH22 pKa = 7.22LDD24 pKa = 3.6DD25 pKa = 4.85SDD27 pKa = 4.46SFSHH31 pKa = 7.28SDD33 pKa = 3.46DD34 pKa = 3.65SDD36 pKa = 4.3SFTHH40 pKa = 7.24PDD42 pKa = 3.51DD43 pKa = 5.02SDD45 pKa = 4.27SFCHH49 pKa = 7.38SDD51 pKa = 4.07DD52 pKa = 4.29SDD54 pKa = 4.33SLTHH58 pKa = 7.24PDD60 pKa = 3.87DD61 pKa = 4.74SDD63 pKa = 4.6SFTHH67 pKa = 7.14PDD69 pKa = 3.29DD70 pKa = 4.91SEE72 pKa = 5.44SFTHH76 pKa = 7.54PDD78 pKa = 3.26DD79 pKa = 3.91TDD81 pKa = 3.92SFSHH85 pKa = 7.06SDD87 pKa = 3.51DD88 pKa = 3.65SDD90 pKa = 4.3SFTHH94 pKa = 7.24PDD96 pKa = 3.37DD97 pKa = 4.98SDD99 pKa = 4.49SFTHH103 pKa = 7.05PDD105 pKa = 3.9DD106 pKa = 5.42SFTHH110 pKa = 7.04PDD112 pKa = 3.39DD113 pKa = 4.98SDD115 pKa = 4.49SFTHH119 pKa = 7.24PDD121 pKa = 3.37DD122 pKa = 4.95SDD124 pKa = 4.2SFTHH128 pKa = 7.01SDD130 pKa = 3.45DD131 pKa = 3.73SDD133 pKa = 4.28SFSHH137 pKa = 7.42PDD139 pKa = 3.33DD140 pKa = 4.75SDD142 pKa = 4.21SFSHH146 pKa = 7.42PDD148 pKa = 3.33DD149 pKa = 4.75SDD151 pKa = 4.21SFSHH155 pKa = 7.42PDD157 pKa = 3.34DD158 pKa = 4.79SDD160 pKa = 4.46SFTHH164 pKa = 7.24PDD166 pKa = 3.36DD167 pKa = 4.82SDD169 pKa = 4.24SFSHH173 pKa = 7.42PDD175 pKa = 3.34DD176 pKa = 4.79SDD178 pKa = 4.46SFTHH182 pKa = 7.24PDD184 pKa = 3.37DD185 pKa = 4.98SDD187 pKa = 4.49SFTHH191 pKa = 7.24PDD193 pKa = 3.37DD194 pKa = 4.95SDD196 pKa = 4.2SFTHH200 pKa = 7.01SDD202 pKa = 3.45DD203 pKa = 3.73SDD205 pKa = 4.28SFSHH209 pKa = 7.42PDD211 pKa = 3.34DD212 pKa = 4.76SDD214 pKa = 4.18SFTHH218 pKa = 7.01SDD220 pKa = 3.45DD221 pKa = 3.73SDD223 pKa = 4.28SFSHH227 pKa = 7.42PDD229 pKa = 3.34DD230 pKa = 4.79SDD232 pKa = 4.46SFTHH236 pKa = 7.24PDD238 pKa = 3.37DD239 pKa = 4.95SDD241 pKa = 4.2SFTHH245 pKa = 7.01SDD247 pKa = 3.45DD248 pKa = 3.73SDD250 pKa = 4.28SFSHH254 pKa = 7.42PDD256 pKa = 3.34DD257 pKa = 4.76SDD259 pKa = 4.18SFTHH263 pKa = 7.01SDD265 pKa = 3.45DD266 pKa = 3.73SDD268 pKa = 4.28SFSHH272 pKa = 7.35PDD274 pKa = 3.26DD275 pKa = 3.97SFF277 pKa = 5.36

Molecular weight: 30.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I4D7L9|A0A2I4D7L9_9TELE next to BRCA1 gene 1 protein OS=Austrofundulus limnaeus OX=52670 GN=nbr1 PE=4 SV=1
MM1 pKa = 7.22AQAVCLKK8 pKa = 10.33SLPDD12 pKa = 3.53NGGDD16 pKa = 3.52GSRR19 pKa = 11.84SRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84GGDD26 pKa = 3.07GSRR29 pKa = 11.84SRR31 pKa = 11.84RR32 pKa = 11.84LRR34 pKa = 11.84NQGRR38 pKa = 11.84RR39 pKa = 11.84THH41 pKa = 6.59GNQGRR46 pKa = 11.84RR47 pKa = 11.84THH49 pKa = 6.72GNQGQRR55 pKa = 11.84THH57 pKa = 6.64GNQGQRR63 pKa = 11.84THH65 pKa = 6.81GDD67 pKa = 2.82QGQRR71 pKa = 11.84THH73 pKa = 7.27RR74 pKa = 11.84NQGQRR79 pKa = 11.84THH81 pKa = 6.61GNQGQRR87 pKa = 11.84THH89 pKa = 6.65GNQGQRR95 pKa = 11.84IHH97 pKa = 7.31RR98 pKa = 11.84NQGQRR103 pKa = 11.84THH105 pKa = 6.54GNQGRR110 pKa = 11.84SRR112 pKa = 11.84RR113 pKa = 11.84ANEE116 pKa = 3.51NQGRR120 pKa = 11.84SRR122 pKa = 11.84SANGNQGRR130 pKa = 11.84SRR132 pKa = 11.84RR133 pKa = 11.84ANGNQGRR140 pKa = 11.84SRR142 pKa = 11.84RR143 pKa = 11.84ANGNQGRR150 pKa = 11.84SRR152 pKa = 11.84RR153 pKa = 11.84ANGNQGRR160 pKa = 11.84SRR162 pKa = 11.84RR163 pKa = 11.84ANGNQGRR170 pKa = 11.84SWRR173 pKa = 11.84ANGNQGRR180 pKa = 11.84SQRR183 pKa = 11.84ANRR186 pKa = 11.84NPSRR190 pKa = 11.84MANRR194 pKa = 11.84NLSRR198 pKa = 11.84RR199 pKa = 11.84ATRR202 pKa = 11.84NQSRR206 pKa = 11.84RR207 pKa = 11.84ATRR210 pKa = 11.84NQSRR214 pKa = 11.84RR215 pKa = 11.84ATRR218 pKa = 11.84NQSRR222 pKa = 11.84RR223 pKa = 11.84ATRR226 pKa = 11.84NQSRR230 pKa = 11.84RR231 pKa = 11.84ATRR234 pKa = 11.84NQSRR238 pKa = 11.84RR239 pKa = 11.84ATRR242 pKa = 11.84NQQ244 pKa = 2.88

Molecular weight: 27.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23690

8758

32448

21847199

31

29191

673.3

74.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.514 ± 0.011

2.132 ± 0.013

5.25 ± 0.008

7.032 ± 0.019

3.48 ± 0.009

6.245 ± 0.017

2.619 ± 0.008

4.13 ± 0.01

5.679 ± 0.014

9.401 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.005

3.81 ± 0.008

5.989 ± 0.017

4.934 ± 0.016

5.657 ± 0.011

9.148 ± 0.018

5.706 ± 0.011

6.273 ± 0.013

1.09 ± 0.005

2.548 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski