Haloactinobacterium album

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Ruaniaceae;

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5FA42|A0A1H5FA42_9MICO PucR C-terminal helix-turn-helix domain-containing protein OS=Haloactinobacterium album OX=648782 GN=SAMN04488554_1279 PE=3 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84RR4 pKa = 11.84ARR6 pKa = 11.84WRR8 pKa = 11.84RR9 pKa = 11.84AAGVFTVALLVATGLAAVEE28 pKa = 4.23ATVAPAPAASAASSPDD44 pKa = 2.94GTGIRR49 pKa = 11.84QSQTFYY55 pKa = 10.97AYY57 pKa = 10.28ADD59 pKa = 3.5AGEE62 pKa = 4.37TVSAAFEE69 pKa = 4.44VVNRR73 pKa = 11.84WSFTGSAVFTVTAPDD88 pKa = 4.34GATQTCTTPAGAALGSVCEE107 pKa = 4.22IGPMTSEE114 pKa = 4.1TSGVWTVDD122 pKa = 2.37VHH124 pKa = 7.59RR125 pKa = 11.84PGDD128 pKa = 3.5FGGAFTWDD136 pKa = 2.92ITVADD141 pKa = 4.94GGTAIPGRR149 pKa = 11.84VWTDD153 pKa = 2.88RR154 pKa = 11.84FLMWNRR160 pKa = 11.84EE161 pKa = 4.06FVDD164 pKa = 3.53LTLWYY169 pKa = 10.17VGASGHH175 pKa = 5.34QYY177 pKa = 9.04EE178 pKa = 4.45VSYY181 pKa = 11.17FDD183 pKa = 3.96FYY185 pKa = 11.39GIEE188 pKa = 4.3SLFSSNQFGVVYY200 pKa = 10.7DD201 pKa = 4.24GTCEE205 pKa = 3.76SAYY208 pKa = 10.52QSAEE212 pKa = 3.88DD213 pKa = 4.36RR214 pKa = 11.84PSGPYY219 pKa = 10.13YY220 pKa = 10.15DD221 pKa = 5.38DD222 pKa = 4.44HH223 pKa = 8.22LSNSGQEE230 pKa = 4.33CGPLYY235 pKa = 11.36NMFFAPPAADD245 pKa = 4.22LPASAADD252 pKa = 3.52ATGATQFILPPVQPPTVSEE271 pKa = 4.12LRR273 pKa = 11.84FTPDD277 pKa = 2.79TPDD280 pKa = 3.09TRR282 pKa = 11.84EE283 pKa = 3.94GTFSFEE289 pKa = 4.2TNHH292 pKa = 6.12VGNADD297 pKa = 4.1LLIDD301 pKa = 4.02ADD303 pKa = 4.5GNGSYY308 pKa = 11.03DD309 pKa = 3.55DD310 pKa = 4.02TVDD313 pKa = 2.98RR314 pKa = 11.84TIPVSVNTGAEE325 pKa = 3.82PSVAFDD331 pKa = 3.79GLDD334 pKa = 3.59GEE336 pKa = 5.33GNAISPSTRR345 pKa = 11.84FNATMRR351 pKa = 11.84ITQAGEE357 pKa = 3.45IHH359 pKa = 6.4FVNGDD364 pKa = 3.3VEE366 pKa = 4.65SRR368 pKa = 11.84GGISVTALTGTADD381 pKa = 3.88GTSTLYY387 pKa = 10.57WNDD390 pKa = 3.53SEE392 pKa = 4.99LQTEE396 pKa = 4.54EE397 pKa = 5.13RR398 pKa = 11.84DD399 pKa = 3.91QSCLPPVLDD408 pKa = 4.73GRR410 pKa = 11.84DD411 pKa = 3.9GQDD414 pKa = 2.83SSVSGGVHH422 pKa = 6.54GWPCTPRR429 pKa = 11.84NPNDD433 pKa = 3.56GVTGPWGDD441 pKa = 3.43VRR443 pKa = 11.84LIDD446 pKa = 4.27DD447 pKa = 3.61WTFNPVDD454 pKa = 3.52VSASVEE460 pKa = 3.87IPAAEE465 pKa = 4.06GNYY468 pKa = 9.68VVTKK472 pKa = 10.75SSDD475 pKa = 3.82PEE477 pKa = 4.68PGTAVHH483 pKa = 6.77PGDD486 pKa = 3.46VVTYY490 pKa = 8.45TIEE493 pKa = 3.9VAQIGNDD500 pKa = 3.19SAAANVSDD508 pKa = 5.1DD509 pKa = 4.41LSDD512 pKa = 3.9VLDD515 pKa = 4.32DD516 pKa = 4.41ASYY519 pKa = 11.51NGDD522 pKa = 3.28ASATAGEE529 pKa = 4.23VDD531 pKa = 3.74VTGTTLNWNGTLLDD545 pKa = 4.29GGSARR550 pKa = 11.84ITYY553 pKa = 10.29SVTVDD558 pKa = 3.45DD559 pKa = 5.57AGDD562 pKa = 3.46VSSGDD567 pKa = 3.56GQVGNVVTSPGCGSDD582 pKa = 3.21ACATEE587 pKa = 4.73HH588 pKa = 6.74PVGDD592 pKa = 3.74YY593 pKa = 10.8SVEE596 pKa = 4.04KK597 pKa = 10.39SAEE600 pKa = 4.03PAPGSAVTDD609 pKa = 4.03GDD611 pKa = 4.09TVAYY615 pKa = 8.03TLTVAQEE622 pKa = 3.68GAAAVEE628 pKa = 4.62GASLVDD634 pKa = 4.34DD635 pKa = 5.02LSDD638 pKa = 3.78VLDD641 pKa = 4.24DD642 pKa = 4.68AVWNGDD648 pKa = 3.48LVASAGAAEE657 pKa = 4.25FDD659 pKa = 3.85EE660 pKa = 5.23DD661 pKa = 3.85AQEE664 pKa = 4.31LRR666 pKa = 11.84WSGDD670 pKa = 3.18LGIDD674 pKa = 3.51DD675 pKa = 4.95VVTISYY681 pKa = 10.85SVMVTGSGDD690 pKa = 3.38TSVDD694 pKa = 3.12NTVASDD700 pKa = 3.84GCGTAEE706 pKa = 4.31EE707 pKa = 4.92CQSSHH712 pKa = 7.19PYY714 pKa = 9.86GGYY717 pKa = 10.55GVVKK721 pKa = 10.36SADD724 pKa = 3.64PAPGGGVAEE733 pKa = 4.82GEE735 pKa = 4.55TVAYY739 pKa = 8.26TLTVTQSGQGSVPAEE754 pKa = 4.02LSDD757 pKa = 5.13DD758 pKa = 3.73LTAVLDD764 pKa = 4.0DD765 pKa = 4.49AVYY768 pKa = 11.19NDD770 pKa = 5.08DD771 pKa = 4.17LQASAGAASYY781 pKa = 10.79DD782 pKa = 4.13SEE784 pKa = 5.13SGTIDD789 pKa = 2.87WDD791 pKa = 3.63GTLARR796 pKa = 11.84GDD798 pKa = 3.88VVTISYY804 pKa = 10.54SVTVQPVGTGDD815 pKa = 3.16GSLRR819 pKa = 11.84NVVTSPGCDD828 pKa = 2.96SDD830 pKa = 4.36ADD832 pKa = 3.92CVTEE836 pKa = 3.79NRR838 pKa = 11.84VGGFVYY844 pKa = 10.52SKK846 pKa = 10.69SADD849 pKa = 3.58GGPGSQVSQGDD860 pKa = 3.98VITYY864 pKa = 10.08SVTVTHH870 pKa = 6.93TGAGPVAEE878 pKa = 4.76ARR880 pKa = 11.84VSDD883 pKa = 4.33DD884 pKa = 3.64LSGVLDD890 pKa = 4.4DD891 pKa = 5.26ATFNDD896 pKa = 4.46DD897 pKa = 3.65ASASSGSIRR906 pKa = 11.84VDD908 pKa = 3.0GSTLDD913 pKa = 3.49WTGSLAPGDD922 pKa = 3.96VVEE925 pKa = 4.51ITYY928 pKa = 10.86SVTVTGAGDD937 pKa = 3.6LAVDD941 pKa = 3.83NVVLTDD947 pKa = 4.15DD948 pKa = 4.16PRR950 pKa = 11.84GVCDD954 pKa = 4.75PDD956 pKa = 3.45ATCATEE962 pKa = 4.26HH963 pKa = 5.74QVPPSPDD970 pKa = 3.24LPATGARR977 pKa = 11.84VGAWVIPAALLMTGLGALVLMGARR1001 pKa = 11.84RR1002 pKa = 11.84HH1003 pKa = 5.5RR1004 pKa = 11.84AAEE1007 pKa = 4.02KK1008 pKa = 10.58

Molecular weight: 103.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5LNV2|A0A1H5LNV2_9MICO TIR domain-containing protein OS=Haloactinobacterium album OX=648782 GN=SAMN04488554_2902 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.97GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4192

0

4192

1448774

29

2441

345.6

37.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.017 ± 0.05

0.611 ± 0.009

6.407 ± 0.043

5.96 ± 0.036

2.844 ± 0.022

9.119 ± 0.042

2.261 ± 0.021

3.902 ± 0.026

1.314 ± 0.022

10.074 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.895 ± 0.016

1.876 ± 0.019

5.764 ± 0.03

2.994 ± 0.017

7.247 ± 0.052

5.488 ± 0.027

6.569 ± 0.037

8.957 ± 0.038

1.645 ± 0.019

2.057 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski