Circovirus-like genome RW-C

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GII0|C6GII0_9VIRU Uncharacterized protein OS=Circovirus-like genome RW-C OX=642253 PE=3 SV=1
MM1 pKa = 7.57NKK3 pKa = 10.15RR4 pKa = 11.84IRR6 pKa = 11.84NVCFTGFNEE15 pKa = 4.0TGYY18 pKa = 11.04DD19 pKa = 3.62FNEE22 pKa = 4.01NQMKK26 pKa = 9.94YY27 pKa = 10.45LIVGKK32 pKa = 8.01EE33 pKa = 3.66ICPTTGKK40 pKa = 8.92EE41 pKa = 3.43HH42 pKa = 5.25WQGYY46 pKa = 8.5VEE48 pKa = 5.03LKK50 pKa = 9.49NAKK53 pKa = 8.49TFSAIKK59 pKa = 10.45KK60 pKa = 9.16LFKK63 pKa = 10.46DD64 pKa = 3.53NKK66 pKa = 9.78LHH68 pKa = 7.09LEE70 pKa = 3.97EE71 pKa = 5.15RR72 pKa = 11.84KK73 pKa = 10.29ASAQCASNYY82 pKa = 9.24CKK84 pKa = 10.51KK85 pKa = 10.46EE86 pKa = 4.05GNFKK90 pKa = 10.71EE91 pKa = 4.09FGKK94 pKa = 10.42ISEE97 pKa = 4.18QGKK100 pKa = 8.03RR101 pKa = 11.84TDD103 pKa = 4.81LIEE106 pKa = 4.56LKK108 pKa = 10.82DD109 pKa = 4.97DD110 pKa = 3.21ILAGKK115 pKa = 9.39KK116 pKa = 9.51NCTEE120 pKa = 3.75VRR122 pKa = 11.84EE123 pKa = 4.31EE124 pKa = 4.01NPEE127 pKa = 4.37LYY129 pKa = 9.79HH130 pKa = 7.03QYY132 pKa = 11.06GRR134 pKa = 11.84TLDD137 pKa = 3.85KK138 pKa = 11.66LEE140 pKa = 5.27DD141 pKa = 4.96DD142 pKa = 4.53YY143 pKa = 12.14LCKK146 pKa = 10.21QKK148 pKa = 10.84RR149 pKa = 11.84NWMTKK154 pKa = 10.08GIWIWGPTGCGKK166 pKa = 10.4SRR168 pKa = 11.84WAYY171 pKa = 6.25EE172 pKa = 4.31TYY174 pKa = 10.48PNAYY178 pKa = 7.61TWADD182 pKa = 3.48DD183 pKa = 5.63KK184 pKa = 11.63DD185 pKa = 3.73WQDD188 pKa = 3.72CYY190 pKa = 11.26SGQEE194 pKa = 4.04TIIIDD199 pKa = 3.86DD200 pKa = 3.5FRR202 pKa = 11.84GNIKK206 pKa = 9.86FQNLLKK212 pKa = 10.46MIDD215 pKa = 3.33RR216 pKa = 11.84YY217 pKa = 8.97EE218 pKa = 4.24FKK220 pKa = 10.39LSRR223 pKa = 11.84RR224 pKa = 11.84GRR226 pKa = 11.84VPFPLLAKK234 pKa = 9.37TIIITSSMPPEE245 pKa = 3.83KK246 pKa = 10.41VYY248 pKa = 11.4KK249 pKa = 10.12NLSVDD254 pKa = 4.43DD255 pKa = 5.12SIEE258 pKa = 3.87QLKK261 pKa = 10.22RR262 pKa = 11.84RR263 pKa = 11.84CKK265 pKa = 10.16IIEE268 pKa = 4.08LPKK271 pKa = 10.08CTEE274 pKa = 3.69EE275 pKa = 4.35NYY277 pKa = 9.66TEE279 pKa = 4.24VVRR282 pKa = 11.84VILEE286 pKa = 3.89NSVV289 pKa = 2.82

Molecular weight: 33.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GII0|C6GII0_9VIRU Uncharacterized protein OS=Circovirus-like genome RW-C OX=642253 PE=3 SV=1
MM1 pKa = 7.81PYY3 pKa = 9.58YY4 pKa = 10.17RR5 pKa = 11.84KK6 pKa = 10.07KK7 pKa = 10.06RR8 pKa = 11.84VYY10 pKa = 9.66RR11 pKa = 11.84KK12 pKa = 9.3KK13 pKa = 10.43RR14 pKa = 11.84PMRR17 pKa = 11.84RR18 pKa = 11.84NRR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.52YY23 pKa = 9.23SRR25 pKa = 11.84LPRR28 pKa = 11.84RR29 pKa = 11.84SLGNPNQKK37 pKa = 9.61VYY39 pKa = 10.44YY40 pKa = 9.4FKK42 pKa = 11.1RR43 pKa = 11.84FTTLGTIVVTSSSANAYY60 pKa = 7.42GASVFSLDD68 pKa = 3.43QLPGYY73 pKa = 8.49TEE75 pKa = 3.94FTNLFDD81 pKa = 4.42FYY83 pKa = 10.72KK84 pKa = 10.43IKK86 pKa = 10.6AIKK89 pKa = 10.28LSFIPTSNVTLQTGNSSTTVANSIYY114 pKa = 10.02NNRR117 pKa = 11.84IFTVIDD123 pKa = 3.53YY124 pKa = 10.36NDD126 pKa = 3.5AGIPTSVNEE135 pKa = 3.55LRR137 pKa = 11.84EE138 pKa = 3.92YY139 pKa = 11.27SNCKK143 pKa = 8.93WSPNNKK149 pKa = 6.86IHH151 pKa = 7.02KK152 pKa = 9.73RR153 pKa = 11.84YY154 pKa = 9.5IVPNPLADD162 pKa = 3.72ATDD165 pKa = 4.17DD166 pKa = 3.88STISLMNKK174 pKa = 8.64PWVPTTNYY182 pKa = 10.82AMDD185 pKa = 3.99YY186 pKa = 10.8YY187 pKa = 10.61AIKK190 pKa = 9.52WAIEE194 pKa = 3.6NTAAGVGVEE203 pKa = 4.3LYY205 pKa = 11.03KK206 pKa = 10.22MEE208 pKa = 4.38AKK210 pKa = 10.4FYY212 pKa = 10.19IACKK216 pKa = 10.07SPKK219 pKa = 10.07

Molecular weight: 25.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

508

219

289

254.0

29.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.921 ± 1.149

2.362 ± 0.863

4.921 ± 0.756

6.299 ± 2.121

3.937 ± 0.103

4.921 ± 0.756

0.787 ± 0.197

6.89 ± 0.296

10.433 ± 1.047

6.496 ± 0.606

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.969 ± 0.187

7.283 ± 0.83

4.331 ± 0.957

2.559 ± 0.709

6.102 ± 0.445

5.906 ± 1.379

6.89 ± 0.792

4.724 ± 0.722

1.969 ± 0.357

6.299 ± 1.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski