Lysobacter silvestris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K1Q089|A0A2K1Q089_9GAMM Putative transcriptional regulator OS=Lysobacter silvestris OX=1645665 GN=Lysil_0087 PE=4 SV=1
MM1 pKa = 7.68SEE3 pKa = 4.08LAVAADD9 pKa = 3.81TPFRR13 pKa = 11.84TWMCVVCGFIYY24 pKa = 10.64DD25 pKa = 3.83EE26 pKa = 4.46ALGLPEE32 pKa = 4.87EE33 pKa = 4.64GLAAGTRR40 pKa = 11.84WEE42 pKa = 5.6DD43 pKa = 4.49IPDD46 pKa = 3.14TWTCPDD52 pKa = 3.91CGVTKK57 pKa = 10.68DD58 pKa = 3.96DD59 pKa = 5.22FEE61 pKa = 4.29MTEE64 pKa = 3.7II65 pKa = 4.61

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K1PZE9|A0A2K1PZE9_9GAMM Uncharacterized protein OS=Lysobacter silvestris OX=1645665 GN=Lysil_2340 PE=4 SV=1
MM1 pKa = 7.58NIGATARR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 4.6GTTALLSLAIAGFGTATALAGSPVAQAVAPVAAVKK45 pKa = 10.05AHH47 pKa = 5.74PTSGKK52 pKa = 9.2ASRR55 pKa = 11.84IVRR58 pKa = 11.84IRR60 pKa = 11.84QAQAVPFFAFPPRR73 pKa = 11.84II74 pKa = 3.66

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2544

0

2544

874491

30

3684

343.7

37.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.369 ± 0.069

0.847 ± 0.016

6.126 ± 0.038

4.964 ± 0.053

3.401 ± 0.031

8.471 ± 0.078

2.436 ± 0.028

4.717 ± 0.032

3.228 ± 0.045

10.267 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.027

2.848 ± 0.052

5.177 ± 0.033

3.72 ± 0.032

7.222 ± 0.062

5.386 ± 0.038

5.235 ± 0.064

7.28 ± 0.042

1.519 ± 0.025

2.387 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski