Emiliania huxleyi virus 207

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Coccolithovirus; unclassified Coccolithovirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 467 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4YBU3|G4YBU3_9PHYC Uncharacterized protein OS=Emiliania huxleyi virus 207 OX=181214 GN=EQVG_00202 PE=4 SV=1
MM1 pKa = 7.24SVCKK5 pKa = 9.37ITNDD9 pKa = 5.27GIDD12 pKa = 3.35LANNVDD18 pKa = 3.48VDD20 pKa = 3.81TLYY23 pKa = 10.92KK24 pKa = 10.23RR25 pKa = 11.84EE26 pKa = 4.05EE27 pKa = 4.27VVIQPPASSLRR38 pKa = 11.84ICLYY42 pKa = 10.46TLDD45 pKa = 4.67KK46 pKa = 10.91PGTPVKK52 pKa = 10.56QSTLLSYY59 pKa = 10.74DD60 pKa = 3.94LPPPFDD66 pKa = 3.56NYY68 pKa = 11.01LYY70 pKa = 10.48NSPVYY75 pKa = 9.16MQFIGGTITSVSQATQLIKK94 pKa = 10.89LLGEE98 pKa = 4.36IFVHH102 pKa = 6.39TDD104 pKa = 3.19DD105 pKa = 5.45SVSDD109 pKa = 4.12SNTLVYY115 pKa = 10.62EE116 pKa = 4.0KK117 pKa = 11.17VEE119 pKa = 4.08DD120 pKa = 3.9VEE122 pKa = 4.88SEE124 pKa = 4.18EE125 pKa = 4.89EE126 pKa = 3.97EE127 pKa = 4.42DD128 pKa = 5.13DD129 pKa = 4.64VPEE132 pKa = 4.38EE133 pKa = 4.38YY134 pKa = 10.56YY135 pKa = 11.14DD136 pKa = 3.68HH137 pKa = 7.26NEE139 pKa = 3.67GSEE142 pKa = 3.99YY143 pKa = 11.02EE144 pKa = 4.05EE145 pKa = 5.84DD146 pKa = 3.54EE147 pKa = 4.33DD148 pKa = 4.59VAYY151 pKa = 10.95SDD153 pKa = 6.17DD154 pKa = 4.07EE155 pKa = 6.76DD156 pKa = 5.97DD157 pKa = 5.8DD158 pKa = 4.29EE159 pKa = 6.32SKK161 pKa = 11.27VEE163 pKa = 4.23DD164 pKa = 3.69SSTVYY169 pKa = 10.88EE170 pKa = 4.89DD171 pKa = 5.17DD172 pKa = 4.73SSLKK176 pKa = 10.73

Molecular weight: 19.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4YCE8|G4YCE8_9PHYC Uncharacterized protein OS=Emiliania huxleyi virus 207 OX=181214 GN=EQVG_00408 PE=4 SV=1
MM1 pKa = 7.25AVSRR5 pKa = 11.84RR6 pKa = 11.84SRR8 pKa = 11.84YY9 pKa = 7.36GTVRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84TVRR20 pKa = 11.84KK21 pKa = 8.12STASRR26 pKa = 11.84STKK29 pKa = 9.49VGRR32 pKa = 11.84VAKK35 pKa = 9.99LSNMSAGCRR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 10.33VKK48 pKa = 10.58SKK50 pKa = 10.47LGSRR54 pKa = 11.84KK55 pKa = 9.56LSRR58 pKa = 11.84ATRR61 pKa = 11.84ASIIRR66 pKa = 11.84SSVKK70 pKa = 8.95SWKK73 pKa = 10.17RR74 pKa = 11.84MSKK77 pKa = 7.59TRR79 pKa = 11.84KK80 pKa = 8.61AAYY83 pKa = 9.3SKK85 pKa = 10.35RR86 pKa = 11.84GRR88 pKa = 11.84VSTRR92 pKa = 11.84SRR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84STKK99 pKa = 8.68RR100 pKa = 11.84SRR102 pKa = 11.84RR103 pKa = 11.84TRR105 pKa = 11.84TKK107 pKa = 10.01RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84STKK113 pKa = 9.18RR114 pKa = 11.84SRR116 pKa = 11.84RR117 pKa = 11.84SKK119 pKa = 7.88RR120 pKa = 11.84TKK122 pKa = 10.09RR123 pKa = 11.84RR124 pKa = 11.84SSTLKK129 pKa = 10.23RR130 pKa = 11.84PKK132 pKa = 9.57TSTMSKK138 pKa = 10.11SLKK141 pKa = 10.19KK142 pKa = 10.61YY143 pKa = 10.13IRR145 pKa = 11.84TKK147 pKa = 10.44SGKK150 pKa = 9.72KK151 pKa = 9.61NLSKK155 pKa = 11.04VSTKK159 pKa = 7.72MLRR162 pKa = 11.84KK163 pKa = 9.83LIAMWRR169 pKa = 11.84RR170 pKa = 11.84MSAKK174 pKa = 9.61SKK176 pKa = 10.22ARR178 pKa = 11.84YY179 pKa = 8.53SVRR182 pKa = 11.84STRR185 pKa = 11.84KK186 pKa = 7.96RR187 pKa = 11.84STRR190 pKa = 11.84KK191 pKa = 8.83RR192 pKa = 11.84STRR195 pKa = 11.84KK196 pKa = 8.83RR197 pKa = 11.84STRR200 pKa = 11.84KK201 pKa = 8.83RR202 pKa = 11.84STRR205 pKa = 11.84KK206 pKa = 8.83RR207 pKa = 11.84STRR210 pKa = 11.84KK211 pKa = 8.83RR212 pKa = 11.84STRR215 pKa = 11.84KK216 pKa = 8.83RR217 pKa = 11.84STRR220 pKa = 11.84KK221 pKa = 8.83RR222 pKa = 11.84STRR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 11.84RR228 pKa = 11.84RR229 pKa = 11.84TTRR232 pKa = 11.84KK233 pKa = 9.12RR234 pKa = 11.84RR235 pKa = 11.84RR236 pKa = 11.84SRR238 pKa = 11.84PRR240 pKa = 11.84TRR242 pKa = 11.84RR243 pKa = 11.84VRR245 pKa = 11.84RR246 pKa = 11.84TRR248 pKa = 11.84RR249 pKa = 11.84TRR251 pKa = 11.84RR252 pKa = 11.84TRR254 pKa = 11.84RR255 pKa = 11.84TRR257 pKa = 11.84RR258 pKa = 11.84TVRR261 pKa = 11.84RR262 pKa = 11.84RR263 pKa = 3.12

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

466

1

467

123462

49

5427

264.4

29.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.398 ± 0.116

1.87 ± 0.075

5.708 ± 0.085

5.08 ± 0.126

3.865 ± 0.077

5.43 ± 0.159

2.535 ± 0.111

7.308 ± 0.126

5.592 ± 0.21

7.353 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.019 ± 0.09

5.564 ± 0.089

6.017 ± 0.412

3.063 ± 0.056

4.764 ± 0.119

7.521 ± 0.158

7.023 ± 0.133

6.565 ± 0.09

1.07 ± 0.04

4.255 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski