Edwardsiella phage IW-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kafunavirus; Edwardsiella virus KF1; Edwardsiella phage KF-1

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4PWS9|K4PWS9_9CAUD Putative terminase large subunit OS=Edwardsiella phage IW-1 OX=1244857 PE=4 SV=1
MM1 pKa = 7.21SRR3 pKa = 11.84VRR5 pKa = 11.84QIIADD10 pKa = 3.66ARR12 pKa = 11.84YY13 pKa = 10.39SLADD17 pKa = 3.53PKK19 pKa = 11.05GEE21 pKa = 3.69RR22 pKa = 11.84WTDD25 pKa = 3.16EE26 pKa = 3.68RR27 pKa = 11.84LLRR30 pKa = 11.84LLSQGQKK37 pKa = 10.84DD38 pKa = 3.58LARR41 pKa = 11.84EE42 pKa = 4.01LKK44 pKa = 10.32LLKK47 pKa = 10.68AEE49 pKa = 4.23TSLALSPGQAMYY61 pKa = 11.09KK62 pKa = 10.26LPEE65 pKa = 4.77DD66 pKa = 2.99LWLITRR72 pKa = 11.84AAFSLVRR79 pKa = 11.84IPLLSYY85 pKa = 11.08DD86 pKa = 4.32AMDD89 pKa = 4.09SADD92 pKa = 3.8AAWFARR98 pKa = 11.84TGNRR102 pKa = 11.84VEE104 pKa = 3.93NLVYY108 pKa = 10.69DD109 pKa = 4.48LRR111 pKa = 11.84NVDD114 pKa = 4.82TIRR117 pKa = 11.84VWPTPDD123 pKa = 4.31DD124 pKa = 5.96DD125 pKa = 3.93IDD127 pKa = 4.92KK128 pKa = 10.74DD129 pKa = 4.05VYY131 pKa = 10.31VFEE134 pKa = 4.89GEE136 pKa = 4.36GVLVGTMDD144 pKa = 3.53TAYY147 pKa = 10.31GVYY150 pKa = 10.55ADD152 pKa = 4.75APIDD156 pKa = 3.68KK157 pKa = 10.64VNGVLASLDD166 pKa = 3.51EE167 pKa = 4.37LASFNSVYY175 pKa = 10.79GVTADD180 pKa = 4.62LMRR183 pKa = 11.84NTLAVRR189 pKa = 11.84NTAPYY194 pKa = 9.52FGVVTEE200 pKa = 4.48FDD202 pKa = 3.73GLVPEE207 pKa = 4.66PVFGVLSDD215 pKa = 3.39IVEE218 pKa = 4.42SDD220 pKa = 4.0TIATFDD226 pKa = 3.84SLFGVATDD234 pKa = 3.66VADD237 pKa = 3.4VTGAVTIQYY246 pKa = 10.12IKK248 pKa = 11.08DD249 pKa = 3.67PDD251 pKa = 4.08DD252 pKa = 3.59VLSLDD257 pKa = 4.13GEE259 pKa = 4.61LGVPRR264 pKa = 11.84VFDD267 pKa = 3.65KK268 pKa = 11.46ALVYY272 pKa = 9.43YY273 pKa = 9.97VIGHH277 pKa = 7.22AFSDD281 pKa = 4.47DD282 pKa = 4.83LDD284 pKa = 3.72TQYY287 pKa = 10.89QQKK290 pKa = 10.3SDD292 pKa = 3.68RR293 pKa = 11.84ALSMYY298 pKa = 10.52ARR300 pKa = 11.84EE301 pKa = 4.12LTNIGEE307 pKa = 4.25QTRR310 pKa = 11.84EE311 pKa = 3.73YY312 pKa = 10.67DD313 pKa = 3.23ASATTKK319 pKa = 10.43YY320 pKa = 10.48NRR322 pKa = 11.84SSYY325 pKa = 10.64RR326 pKa = 11.84GAFDD330 pKa = 4.44DD331 pKa = 4.36

Molecular weight: 36.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4PW89|K4PW89_9CAUD Putative DNA polymerase OS=Edwardsiella phage IW-1 OX=1244857 PE=3 SV=1
MM1 pKa = 7.12LTAKK5 pKa = 9.95PHH7 pKa = 5.15YY8 pKa = 9.87KK9 pKa = 10.39KK10 pKa = 10.03LTSEE14 pKa = 3.84QLNAIKK20 pKa = 10.16QHH22 pKa = 5.7VPSNTNFTPTGSVEE36 pKa = 4.19LNRR39 pKa = 11.84TINLAKK45 pKa = 10.4LYY47 pKa = 10.16AQASINYY54 pKa = 9.08INGAWYY60 pKa = 9.67EE61 pKa = 4.37VTWKK65 pKa = 10.82AA66 pKa = 4.3

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

13171

40

1168

274.4

30.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.582 ± 0.413

1.063 ± 0.135

6.264 ± 0.247

5.899 ± 0.343

3.462 ± 0.203

7.289 ± 0.374

1.617 ± 0.185

4.707 ± 0.207

6.044 ± 0.345

8.989 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.205

4.472 ± 0.156

3.682 ± 0.232

4.252 ± 0.256

4.783 ± 0.268

6.256 ± 0.285

6.249 ± 0.321

7.532 ± 0.358

1.298 ± 0.137

3.994 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski