Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Simian immunodeficiency virus; Simian immunodeficiency virus - sm

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P19509|VPR_SIVSP Protein Vpr OS=Simian immunodeficiency virus (isolate PBj14/BCL-3) OX=11738 GN=vpr PE=1 SV=1
MM1 pKa = 7.48TEE3 pKa = 4.53RR4 pKa = 11.84PPEE7 pKa = 4.27DD8 pKa = 3.58EE9 pKa = 4.35APQRR13 pKa = 11.84EE14 pKa = 4.52PWDD17 pKa = 3.42EE18 pKa = 3.85WVVEE22 pKa = 3.95VLEE25 pKa = 4.63EE26 pKa = 4.14IKK28 pKa = 10.98EE29 pKa = 3.96EE30 pKa = 4.04ALNHH34 pKa = 6.58FDD36 pKa = 4.72PRR38 pKa = 11.84LLTALGNYY46 pKa = 9.66IYY48 pKa = 10.21DD49 pKa = 3.55RR50 pKa = 11.84HH51 pKa = 6.43GDD53 pKa = 3.6TLEE56 pKa = 4.37GAGEE60 pKa = 4.94LIRR63 pKa = 11.84ILQRR67 pKa = 11.84ALFIHH72 pKa = 6.25FRR74 pKa = 11.84GGCRR78 pKa = 11.84HH79 pKa = 5.46SRR81 pKa = 11.84IGQSGGGNPLSTIPPSRR98 pKa = 11.84GVLL101 pKa = 3.39

Molecular weight: 11.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P19503|ENV_SIVSP Envelope glycoprotein gp160 OS=Simian immunodeficiency virus (isolate PBj14/BCL-3) OX=11738 GN=env PE=3 SV=1
MM1 pKa = 7.52SSNEE5 pKa = 3.6EE6 pKa = 3.69EE7 pKa = 3.92LRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84LRR13 pKa = 11.84LIHH16 pKa = 6.83LLHH19 pKa = 5.75QTNPYY24 pKa = 9.4PDD26 pKa = 4.1GPGTANQRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84WRR42 pKa = 11.84QRR44 pKa = 11.84WQQILALADD53 pKa = 4.78RR54 pKa = 11.84IYY56 pKa = 10.92SFPDD60 pKa = 3.51PPVDD64 pKa = 3.63TPLDD68 pKa = 3.58LAIQQLQRR76 pKa = 11.84LAIEE80 pKa = 4.46EE81 pKa = 4.59LPNPPASAPEE91 pKa = 4.03PLKK94 pKa = 11.06DD95 pKa = 3.61AAEE98 pKa = 4.32SPP100 pKa = 3.94

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3761

100

1449

417.9

47.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.302 ± 0.316

2.34 ± 0.396

3.749 ± 0.313

7.525 ± 0.61

2.606 ± 0.19

7.498 ± 0.589

2.074 ± 0.316

5.504 ± 0.607

6.514 ± 0.849

8.721 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.021 ± 0.258

4.493 ± 0.48

5.982 ± 0.592

6.062 ± 0.472

6.328 ± 0.829

4.626 ± 0.672

5.956 ± 0.885

5.717 ± 0.498

2.898 ± 0.452

3.084 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski