Avon-Heathcote Estuary associated circular virus 22

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5I9V1|A0A0C5I9V1_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 22 OX=1618246 PE=4 SV=1
MM1 pKa = 7.24NPIAVYY7 pKa = 10.49EE8 pKa = 4.22FTLKK12 pKa = 10.77KK13 pKa = 9.85QDD15 pKa = 3.52KK16 pKa = 9.57QNEE19 pKa = 3.99NEE21 pKa = 4.4IIKK24 pKa = 10.05ILYY27 pKa = 5.38PTIVKK32 pKa = 10.14KK33 pKa = 10.91ISFQLEE39 pKa = 4.14KK40 pKa = 10.59SDD42 pKa = 3.87EE43 pKa = 4.56GYY45 pKa = 10.29EE46 pKa = 4.14HH47 pKa = 5.79YY48 pKa = 10.16QGRR51 pKa = 11.84ISLVKK56 pKa = 9.42KK57 pKa = 10.23RR58 pKa = 11.84RR59 pKa = 11.84MKK61 pKa = 9.71EE62 pKa = 3.8TLDD65 pKa = 3.17VLKK68 pKa = 10.49PHH70 pKa = 6.82FPDD73 pKa = 2.87IHH75 pKa = 7.55ISPTCNNGLTEE86 pKa = 4.11NFYY89 pKa = 8.12TTKK92 pKa = 10.35EE93 pKa = 4.01DD94 pKa = 3.56TRR96 pKa = 11.84IDD98 pKa = 4.02GPWTEE103 pKa = 3.66KK104 pKa = 10.73SYY106 pKa = 11.42VYY108 pKa = 10.53VPRR111 pKa = 11.84QIRR114 pKa = 11.84EE115 pKa = 4.06IVDD118 pKa = 3.61LKK120 pKa = 10.27PWQKK124 pKa = 11.05SVVLLSRR131 pKa = 11.84IWDD134 pKa = 3.5TRR136 pKa = 11.84TINIIVDD143 pKa = 3.46TEE145 pKa = 4.31GNIGKK150 pKa = 8.28STLTTYY156 pKa = 10.18MGIHH160 pKa = 6.3NLAKK164 pKa = 10.23QIPFCNDD171 pKa = 2.64YY172 pKa = 11.26KK173 pKa = 11.18DD174 pKa = 4.0VLRR177 pKa = 11.84MVCDD181 pKa = 3.95MPTSGCFIIDD191 pKa = 3.41MPRR194 pKa = 11.84AIRR197 pKa = 11.84KK198 pKa = 7.17EE199 pKa = 3.94KK200 pKa = 10.14LYY202 pKa = 11.2QMYY205 pKa = 10.54SAIEE209 pKa = 4.31TIKK212 pKa = 10.67SGYY215 pKa = 10.67AYY217 pKa = 10.11DD218 pKa = 3.71EE219 pKa = 4.7RR220 pKa = 11.84YY221 pKa = 9.75HH222 pKa = 6.91FKK224 pKa = 10.73DD225 pKa = 3.53KK226 pKa = 11.49YY227 pKa = 10.66FDD229 pKa = 4.46CPCIWVFTNIIPDD242 pKa = 3.57EE243 pKa = 4.27TLLSRR248 pKa = 11.84DD249 pKa = 3.23RR250 pKa = 11.84WRR252 pKa = 11.84KK253 pKa = 7.95WEE255 pKa = 4.46IINEE259 pKa = 4.07EE260 pKa = 4.19LKK262 pKa = 11.12KK263 pKa = 10.46FDD265 pKa = 4.59PLMMRR270 pKa = 4.57

Molecular weight: 32.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5I9V1|A0A0C5I9V1_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 22 OX=1618246 PE=4 SV=1
MM1 pKa = 7.88AFRR4 pKa = 11.84KK5 pKa = 8.64FFPRR9 pKa = 11.84KK10 pKa = 7.75KK11 pKa = 8.28TSGRR15 pKa = 11.84KK16 pKa = 7.93ATTPAKK22 pKa = 10.29RR23 pKa = 11.84RR24 pKa = 11.84TAKK27 pKa = 10.42ALPSRR32 pKa = 11.84FPSGRR37 pKa = 11.84KK38 pKa = 7.32TYY40 pKa = 9.77RR41 pKa = 11.84QTRR44 pKa = 11.84AVTRR48 pKa = 11.84VLKK51 pKa = 10.74NISEE55 pKa = 4.43TKK57 pKa = 9.97IQALTPQNAITPSPVEE73 pKa = 3.9VAPTLGPVFFTNFCLGTAPSAWVGPSGSAAFNNLNGFVWPLGSGAAQRR121 pKa = 11.84NGQYY125 pKa = 10.21IYY127 pKa = 11.02LKK129 pKa = 9.52KK130 pKa = 8.79TALNLRR136 pKa = 11.84VAMNASSRR144 pKa = 11.84HH145 pKa = 5.14GPCKK149 pKa = 10.19FRR151 pKa = 11.84VVVYY155 pKa = 9.72KK156 pKa = 10.21EE157 pKa = 3.53RR158 pKa = 11.84RR159 pKa = 11.84NLYY162 pKa = 9.41NVSGGGNPCDD172 pKa = 3.82NLFISQTGDD181 pKa = 2.99SVGFNTISPVAQRR194 pKa = 11.84TMDD197 pKa = 3.71FNTWLVNKK205 pKa = 10.2RR206 pKa = 11.84NYY208 pKa = 10.06QVVKK212 pKa = 10.77DD213 pKa = 4.02MKK215 pKa = 10.58FVLAPEE221 pKa = 4.26TLSALGSTDD230 pKa = 3.21PFNVNKK236 pKa = 10.46GYY238 pKa = 9.46TSSRR242 pKa = 11.84DD243 pKa = 3.02IRR245 pKa = 11.84LSLGHH250 pKa = 5.5FQKK253 pKa = 11.19AKK255 pKa = 10.58FGDD258 pKa = 4.18DD259 pKa = 3.64NTPEE263 pKa = 4.04DD264 pKa = 3.52QMYY267 pKa = 9.52RR268 pKa = 11.84YY269 pKa = 8.89CISVISMPMSSSEE282 pKa = 4.48APHH285 pKa = 6.66SDD287 pKa = 2.42YY288 pKa = 10.11KK289 pKa = 10.88TYY291 pKa = 10.98VNGVVSCIDD300 pKa = 3.18NN301 pKa = 3.58

Molecular weight: 33.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

571

270

301

285.5

32.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.904 ± 1.944

1.926 ± 0.188

5.254 ± 1.136

4.378 ± 1.694

4.729 ± 0.653

5.254 ± 1.224

1.401 ± 0.287

6.83 ± 2.492

8.056 ± 1.003

6.305 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.802 ± 0.338

5.954 ± 0.962

5.954 ± 0.49

3.152 ± 0.121

6.48 ± 0.353

6.83 ± 1.52

7.356 ± 0.203

6.305 ± 0.949

1.576 ± 0.412

4.553 ± 0.639
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski