Candidatus Planktophila limnetica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Candidatus Nanopelagicales; Candidatus Nanopelagicaceae; Candidatus Planktophila

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1326 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249LES1|A0A249LES1_9ACTN Dihydrolipoyl dehydrogenase OS=Candidatus Planktophila limnetica OX=573600 GN=PHILAsVB114_02965 PE=3 SV=1
MM1 pKa = 7.57TYY3 pKa = 10.64VIAQPCIDD11 pKa = 4.64VKK13 pKa = 11.12DD14 pKa = 3.84KK15 pKa = 11.53SCIEE19 pKa = 4.2EE20 pKa = 4.5CPVDD24 pKa = 5.27CIYY27 pKa = 11.01EE28 pKa = 4.32GNRR31 pKa = 11.84MLYY34 pKa = 9.11IQPDD38 pKa = 3.57EE39 pKa = 4.8CVDD42 pKa = 3.84CGACEE47 pKa = 3.85PVCPVEE53 pKa = 4.4AIYY56 pKa = 11.25YY57 pKa = 9.14EE58 pKa = 4.67DD59 pKa = 5.34DD60 pKa = 4.39VPGPWKK66 pKa = 10.54DD67 pKa = 3.65FQKK70 pKa = 10.81VNSEE74 pKa = 4.04FFVEE78 pKa = 4.62LGSPGGAAKK87 pKa = 10.34VGPTSTDD94 pKa = 3.11HH95 pKa = 7.19ADD97 pKa = 3.79VIAAAAKK104 pKa = 10.46SEE106 pKa = 3.99

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249LHG3|A0A249LHG3_9ACTN Putative drug exporter of the RND superfamily OS=Candidatus Planktophila limnetica OX=573600 GN=PHILAsVB114_02940 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.08RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.1KK16 pKa = 8.61HH17 pKa = 4.41GFRR20 pKa = 11.84ARR22 pKa = 11.84MATRR26 pKa = 11.84AGRR29 pKa = 11.84AVLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.61GRR40 pKa = 11.84VRR42 pKa = 11.84LSAA45 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1326

0

1326

418542

45

1503

315.6

34.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.718 ± 0.081

0.737 ± 0.017

5.429 ± 0.049

5.85 ± 0.078

3.721 ± 0.045

8.06 ± 0.057

1.956 ± 0.034

6.775 ± 0.054

5.037 ± 0.057

9.746 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.026

3.236 ± 0.03

4.314 ± 0.038

2.988 ± 0.031

5.309 ± 0.059

6.884 ± 0.058

5.766 ± 0.044

7.764 ± 0.05

1.217 ± 0.027

2.278 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski