Pseudomonas phage Njord

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Uliginvirus; Pseudomonas virus Njord

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GMI3|A0A2S1GMI3_9CAUD Uncharacterized protein OS=Pseudomonas phage Njord OX=2163985 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84MVFTVEE9 pKa = 3.33VDD11 pKa = 3.78QIEE14 pKa = 4.16EE15 pKa = 3.89DD16 pKa = 3.57ADD18 pKa = 3.84RR19 pKa = 11.84QRR21 pKa = 11.84IAEE24 pKa = 4.06QMVDD28 pKa = 3.44LLQAQWCYY36 pKa = 11.25VDD38 pKa = 3.43VALIEE43 pKa = 4.36VEE45 pKa = 4.64GEE47 pKa = 3.91

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GMJ9|A0A2S1GMJ9_9CAUD Recombination endonuclease VII OS=Pseudomonas phage Njord OX=2163985 PE=4 SV=1
MM1 pKa = 7.5SKK3 pKa = 10.27NAQRR7 pKa = 11.84YY8 pKa = 7.06HH9 pKa = 7.25SYY11 pKa = 10.58YY12 pKa = 9.85ATGRR16 pKa = 11.84HH17 pKa = 6.34DD18 pKa = 4.03KK19 pKa = 10.26RR20 pKa = 11.84TGQANRR26 pKa = 11.84AWSIPVKK33 pKa = 10.23YY34 pKa = 9.91RR35 pKa = 11.84RR36 pKa = 11.84AYY38 pKa = 8.64NHH40 pKa = 6.47GFNSYY45 pKa = 7.78NTPEE49 pKa = 4.49PSPLSWGQRR58 pKa = 11.84FLFWIGRR65 pKa = 11.84KK66 pKa = 9.39LGLDD70 pKa = 3.68FQPYY74 pKa = 8.8GVAAA78 pKa = 4.41

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

14129

47

1258

321.1

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.413 ± 0.559

0.977 ± 0.146

6.441 ± 0.259

6.115 ± 0.318

3.871 ± 0.276

7.771 ± 0.328

2.286 ± 0.196

4.792 ± 0.21

5.528 ± 0.348

8.182 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.086 ± 0.202

4.303 ± 0.2

4.31 ± 0.244

4.36 ± 0.342

5.471 ± 0.283

5.202 ± 0.258

6.172 ± 0.307

6.879 ± 0.28

1.493 ± 0.163

3.348 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski