Edwardsiella phage KF-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kafunavirus; Edwardsiella virus KF1

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4PXB9|K4PXB9_9CAUD Uncharacterized protein OS=Edwardsiella phage KF-1 OX=1244856 PE=4 SV=1
MM1 pKa = 7.21SRR3 pKa = 11.84VRR5 pKa = 11.84QIIADD10 pKa = 3.66ARR12 pKa = 11.84YY13 pKa = 10.39SLADD17 pKa = 3.53PKK19 pKa = 11.05GEE21 pKa = 3.69RR22 pKa = 11.84WTDD25 pKa = 3.16EE26 pKa = 3.68RR27 pKa = 11.84LLRR30 pKa = 11.84LLSQGQKK37 pKa = 10.84DD38 pKa = 3.58LARR41 pKa = 11.84EE42 pKa = 4.01LKK44 pKa = 10.32LLKK47 pKa = 10.68AEE49 pKa = 4.23TSLALSPGQAMYY61 pKa = 11.09KK62 pKa = 10.26LPEE65 pKa = 4.77DD66 pKa = 2.99LWLITRR72 pKa = 11.84AAFNLVRR79 pKa = 11.84IPLLSYY85 pKa = 11.08DD86 pKa = 4.32AMDD89 pKa = 4.09SADD92 pKa = 3.8AAWFARR98 pKa = 11.84TGNRR102 pKa = 11.84VEE104 pKa = 3.93NLVYY108 pKa = 10.69DD109 pKa = 4.48LRR111 pKa = 11.84NVDD114 pKa = 4.82TIRR117 pKa = 11.84VWPTPDD123 pKa = 4.31DD124 pKa = 5.96DD125 pKa = 3.93IDD127 pKa = 4.92KK128 pKa = 10.74DD129 pKa = 4.05VYY131 pKa = 10.31VFEE134 pKa = 4.89GEE136 pKa = 4.36GVLVGTMDD144 pKa = 3.53TAYY147 pKa = 10.31GVYY150 pKa = 10.55ADD152 pKa = 4.75APIDD156 pKa = 3.68KK157 pKa = 10.64VNGVLASLDD166 pKa = 3.51EE167 pKa = 4.37LASFNSVYY175 pKa = 10.79GVTADD180 pKa = 4.62LMRR183 pKa = 11.84NTLAVRR189 pKa = 11.84NTAPYY194 pKa = 9.52FGVVTEE200 pKa = 4.48FDD202 pKa = 3.73GLVPEE207 pKa = 4.66PVFGVLSDD215 pKa = 3.39IVEE218 pKa = 4.42SDD220 pKa = 4.0TIATFDD226 pKa = 3.84SLFGVATDD234 pKa = 3.66VADD237 pKa = 3.4VTGAVTIQYY246 pKa = 10.12IKK248 pKa = 11.08DD249 pKa = 3.67PDD251 pKa = 4.08DD252 pKa = 3.59VLSLDD257 pKa = 4.13GEE259 pKa = 4.61LGVPRR264 pKa = 11.84VFDD267 pKa = 3.65KK268 pKa = 11.46ALVYY272 pKa = 9.43YY273 pKa = 9.97VIGHH277 pKa = 7.22AFSDD281 pKa = 4.47DD282 pKa = 4.83LDD284 pKa = 3.72TQYY287 pKa = 10.89QQKK290 pKa = 10.3SDD292 pKa = 3.68RR293 pKa = 11.84ALSMYY298 pKa = 10.52ARR300 pKa = 11.84EE301 pKa = 4.12LTNIGEE307 pKa = 4.25QTRR310 pKa = 11.84EE311 pKa = 3.73YY312 pKa = 10.67DD313 pKa = 3.23ASATTKK319 pKa = 10.43YY320 pKa = 10.48NRR322 pKa = 11.84SSYY325 pKa = 10.64RR326 pKa = 11.84GAFDD330 pKa = 4.44DD331 pKa = 4.36

Molecular weight: 36.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4PWS2|K4PWS2_9CAUD Putative tailspike protein OS=Edwardsiella phage KF-1 OX=1244856 PE=4 SV=1
MM1 pKa = 7.33TPYY4 pKa = 9.4THH6 pKa = 6.68QLEE9 pKa = 4.52LSDD12 pKa = 3.57VALAILRR19 pKa = 11.84EE20 pKa = 4.02NGLAYY25 pKa = 10.47LANEE29 pKa = 3.91EE30 pKa = 4.34RR31 pKa = 11.84TGKK34 pKa = 8.06TLTAILVSEE43 pKa = 4.34KK44 pKa = 10.79SSAEE48 pKa = 3.94RR49 pKa = 11.84ILVVTKK55 pKa = 10.62KK56 pKa = 10.38KK57 pKa = 10.81ALTGWEE63 pKa = 3.87EE64 pKa = 3.47TLAAFKK70 pKa = 10.23HH71 pKa = 5.07AKK73 pKa = 9.54QYY75 pKa = 11.32VVTNYY80 pKa = 9.85HH81 pKa = 5.23QAHH84 pKa = 5.92KK85 pKa = 10.48QGSNFDD91 pKa = 4.31LVILDD96 pKa = 3.85EE97 pKa = 4.17AHH99 pKa = 6.65NYY101 pKa = 9.25ISSFPKK107 pKa = 9.16PGKK110 pKa = 8.83IHH112 pKa = 6.81KK113 pKa = 9.59EE114 pKa = 3.49LMPICKK120 pKa = 9.7GKK122 pKa = 9.62PILYY126 pKa = 10.12ISATPYY132 pKa = 10.35AQGPQMLFNQFKK144 pKa = 10.36LSSYY148 pKa = 9.62SPWYY152 pKa = 9.3KK153 pKa = 10.44HH154 pKa = 6.25SNFYY158 pKa = 10.62SWFRR162 pKa = 11.84VYY164 pKa = 10.82GKK166 pKa = 9.81PYY168 pKa = 8.36TKK170 pKa = 10.0EE171 pKa = 3.55INGINIPQYY180 pKa = 10.71DD181 pKa = 3.78RR182 pKa = 11.84CNTEE186 pKa = 3.68MVLDD190 pKa = 4.16CVKK193 pKa = 10.65HH194 pKa = 6.06LFITKK199 pKa = 8.6TRR201 pKa = 11.84ADD203 pKa = 3.64LGFEE207 pKa = 4.48HH208 pKa = 7.04EE209 pKa = 5.02PEE211 pKa = 4.98DD212 pKa = 3.48KK213 pKa = 11.15LHH215 pKa = 6.68FIEE218 pKa = 5.9LATNTKK224 pKa = 10.17AVYY227 pKa = 9.88NALLKK232 pKa = 10.95DD233 pKa = 3.6KK234 pKa = 10.58LVQLSVGKK242 pKa = 8.47VVCDD246 pKa = 3.53SAAKK250 pKa = 10.41LRR252 pKa = 11.84TTLHH256 pKa = 5.29QLEE259 pKa = 4.82GGTVKK264 pKa = 9.95IDD266 pKa = 3.59NKK268 pKa = 10.71SHH270 pKa = 5.9VLANNEE276 pKa = 3.88KK277 pKa = 8.65IDD279 pKa = 3.89YY280 pKa = 9.7IKK282 pKa = 10.94EE283 pKa = 4.11HH284 pKa = 6.58FGDD287 pKa = 3.73TGKK290 pKa = 10.54LAIMYY295 pKa = 8.62NFIGEE300 pKa = 4.48GVKK303 pKa = 10.48LNQAFKK309 pKa = 10.54HH310 pKa = 5.14AAILQATSFAEE321 pKa = 4.55GVDD324 pKa = 3.22LHH326 pKa = 7.93KK327 pKa = 10.92YY328 pKa = 10.1DD329 pKa = 3.99EE330 pKa = 4.31LVIYY334 pKa = 9.95SQDD337 pKa = 3.23YY338 pKa = 8.17STARR342 pKa = 11.84HH343 pKa = 4.32TQRR346 pKa = 11.84RR347 pKa = 11.84ARR349 pKa = 11.84QCNQKK354 pKa = 9.35RR355 pKa = 11.84DD356 pKa = 3.62KK357 pKa = 10.74PIVVHH362 pKa = 6.66FLLVANAISSQVYY375 pKa = 8.11KK376 pKa = 10.18TVSVNKK382 pKa = 9.73RR383 pKa = 11.84NYY385 pKa = 8.28VDD387 pKa = 4.29SVFEE391 pKa = 3.97RR392 pKa = 11.84TMLL395 pKa = 4.0

Molecular weight: 45.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

13083

40

969

272.6

30.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.593 ± 0.527

1.07 ± 0.143

6.252 ± 0.199

5.916 ± 0.303

3.485 ± 0.21

7.223 ± 0.417

1.582 ± 0.208

4.701 ± 0.219

5.993 ± 0.286

8.989 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.187

4.51 ± 0.167

3.699 ± 0.209

4.257 ± 0.25

4.815 ± 0.242

6.245 ± 0.289

6.26 ± 0.269

7.536 ± 0.342

1.307 ± 0.15

3.967 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski