Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695) (Hybrid yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Millerozyma; Millerozyma farinosa

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|G8YQ21|G8YQ21_PICSO Piso0_000785 protein OS=Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695) OX=559304 GN=Piso0_000785 PE=3 SV=1
MM1 pKa = 6.88VAASKK6 pKa = 11.06ALIVVVLVSTIQALLIDD23 pKa = 4.0RR24 pKa = 11.84TNDD27 pKa = 2.81IEE29 pKa = 5.11KK30 pKa = 10.35RR31 pKa = 11.84ALLSGITSLVSSVEE45 pKa = 3.99DD46 pKa = 3.61LLGGLLSAVEE56 pKa = 4.1AQLEE60 pKa = 4.54CIADD64 pKa = 3.74VVLGDD69 pKa = 4.68DD70 pKa = 4.63GQITTANEE78 pKa = 4.1DD79 pKa = 3.79EE80 pKa = 4.43FTLAVILANGFYY92 pKa = 10.3DD93 pKa = 4.04YY94 pKa = 11.66GSLDD98 pKa = 3.67SFSSLQSTLNSVADD112 pKa = 4.03NDD114 pKa = 4.89PGLLNNNTLANYY126 pKa = 9.86LGVSITDD133 pKa = 3.35NVQGVDD139 pKa = 3.34NKK141 pKa = 11.24ADD143 pKa = 3.47VLTTFIKK150 pKa = 10.66SVIEE154 pKa = 3.91VLDD157 pKa = 4.83DD158 pKa = 3.76NLSSLGGTSVTSDD171 pKa = 3.07TLANAIQEE179 pKa = 4.32KK180 pKa = 10.69AEE182 pKa = 3.86QNGIDD187 pKa = 3.77FSLSEE192 pKa = 4.58DD193 pKa = 3.47ASSDD197 pKa = 3.59SVQLVITDD205 pKa = 3.99SQWLCKK211 pKa = 10.54LLDD214 pKa = 3.72HH215 pKa = 6.89VVDD218 pKa = 4.12GLEE221 pKa = 4.02GLVDD225 pKa = 4.09CVSVTLTNLKK235 pKa = 10.02SVYY238 pKa = 10.49NLVDD242 pKa = 3.45EE243 pKa = 4.85VLGSLCPAASSSSSGVSSTAPSSTSGSSATSAPSGSSTPTSAATSGASGASSATGSGSPSGSSGTSSGSPSSSSTSGSGSPSGASSATGSGSPSGSSGTSSGSPSSSSTSGSGSPSGASSATSSGSPSGSSSATGSGSPSGSGTSGSPSGSGTSGSPSGSSASGASSVTSSGSPSGSSASGSGSSGASSATSSGSPSGASSATGSGASGSGSSSATASGSSSGSGASSVASASATGSGGSGSSGIIGDD491 pKa = 3.84LVGSIEE497 pKa = 4.01GLLGGLLSAVEE508 pKa = 4.1AQLEE512 pKa = 4.54CIADD516 pKa = 3.74VVLGDD521 pKa = 4.68DD522 pKa = 4.63GQITTANEE530 pKa = 4.1DD531 pKa = 3.79EE532 pKa = 4.43FTLAVILANGFYY544 pKa = 10.3DD545 pKa = 4.04YY546 pKa = 11.66GSLDD550 pKa = 3.67SFSSLQSTLNSVADD564 pKa = 4.03NDD566 pKa = 4.89PGLLNNNTLANYY578 pKa = 9.86LGVSITDD585 pKa = 3.35NVQGVDD591 pKa = 3.34NKK593 pKa = 11.24ADD595 pKa = 3.47VLTTFIKK602 pKa = 10.68SVIEE606 pKa = 3.85VLADD610 pKa = 3.49NLSSLGGTSVTSDD623 pKa = 3.07TLANAIQEE631 pKa = 4.32KK632 pKa = 10.69AEE634 pKa = 3.86QNGIDD639 pKa = 3.77FSLSEE644 pKa = 4.58DD645 pKa = 3.47ASSDD649 pKa = 3.59SVQLVITDD657 pKa = 3.99SQWLCKK663 pKa = 10.2LLNHH667 pKa = 5.99VVDD670 pKa = 4.25GLEE673 pKa = 3.96GLVDD677 pKa = 4.09CVSVTLTNLKK687 pKa = 10.02SVYY690 pKa = 10.49NLVDD694 pKa = 3.45EE695 pKa = 4.85VLGSLCPAASSSSSGVSSTAPSSTSGSSATSTPSGSSAPTSAATSGASGASSATGSGTPSGSSTSGSGSSSGASSATSSGSPSGSSASGSGASSATGSGTPSGSSASGSGSSSATGSGSPSGSSGTTSGSPSGSSSATGSGSPSGSSASGSGSASGASSATGSGASGSGSSSATGSGSPSGSGASGSGASSATGSGSPSSSSASGASSATSSGASSASSATGSPSGSGSSSATASGSSSGSGTSSVASASATGSGGSGSSGIIGDD960 pKa = 3.84LVGSIEE966 pKa = 4.13GLLSGLLSAVEE977 pKa = 4.51SKK979 pKa = 10.93LEE981 pKa = 4.38CIADD985 pKa = 3.68VVLGDD990 pKa = 4.15NPQSTSATEE999 pKa = 3.83DD1000 pKa = 3.54EE1001 pKa = 5.0YY1002 pKa = 11.24ILAVILANGFYY1013 pKa = 10.28DD1014 pKa = 4.16YY1015 pKa = 11.21RR1016 pKa = 11.84SLDD1019 pKa = 3.59SFDD1022 pKa = 4.64SLQSTLNSVAEE1033 pKa = 4.19NDD1035 pKa = 4.2PDD1037 pKa = 4.05LLNNNTLANYY1047 pKa = 9.86LGVSITDD1054 pKa = 3.35NVQGVDD1060 pKa = 3.34NKK1062 pKa = 11.24ADD1064 pKa = 3.47VLTTFIKK1071 pKa = 10.68SVIEE1075 pKa = 3.85VLADD1079 pKa = 3.49NLSSLGGTSVTSDD1092 pKa = 3.07TLANAIQEE1100 pKa = 4.32KK1101 pKa = 10.69AEE1103 pKa = 3.86QNGIDD1108 pKa = 3.77FSLSEE1113 pKa = 4.58DD1114 pKa = 3.47ASSDD1118 pKa = 3.59SVQLVITDD1126 pKa = 3.99SQWLCKK1132 pKa = 10.2LLNHH1136 pKa = 5.99VVDD1139 pKa = 4.16GLEE1142 pKa = 4.14GLVQCTVVDD1151 pKa = 4.56LFDD1154 pKa = 4.52LKK1156 pKa = 10.93SVYY1159 pKa = 10.87NLVDD1163 pKa = 3.45EE1164 pKa = 4.85VLGSLCPTASSSSSAVSSTAPSSTSGSSATSAPSGSSTPTSAATSGASGASSASGSGSSGSGSPSGSGASSATSSGSTSGSGASGSGSSSATSSGSPSGSGASGASGSSSATGSATGSGSPSGSSGTSSGSPSGASSATGSGASGSGSSSATGSGSPSGSGASGSGSSSATSSGSPSGSSTSGSSASGASSATGSGSPSGTSSSSASGASSATSSGSPSGSGASGSGSSSATSSGSPSGSGASGSGSSSATSSGSSSGSGASGASGSSSATGSGSSSGSGASSATSTGSPSGSGSSGSGSSSATGSTTGSGSPSGSGASGSGSSSATSSGSPSGASSATGSGASGSGSSSATGSGSPSGSGASGSGSSSATSSGSPSGSGASSATSSGSTSGSGASGSGSSSATSSGSPSGSGASGASGSSSATGSATGSGSPSGSSGTSSGSPSGASSATGSGASGSGSSSATGSGSPSGSGASGSGSSSATSSGSPSGSSTSGSSASGASSATGSGSPSGTSSSSASGASSATSSGSPSGSGASGSGSSSATSSGSSSGSGASGASGSSSATGSGSSSGSGASSATSTGSPSGSGSSGSGSSSATGSTTGSGSPSGSGASGSGSSSATSSGSPSGSGASGASGSSSATGSATGSGSPSGSSGTSSGSPSGSSASGASSASGTGASGASGSSSATSSGSASGTGASGSGSSSATGSATGSGSASGSGASSVASASATGSGGSGSSGLIGDD1905 pKa = 4.63LVGSIEE1911 pKa = 4.13GLLSGLLSAVEE1922 pKa = 4.46SKK1924 pKa = 10.98LEE1926 pKa = 4.36SIAEE1930 pKa = 4.18VVLGDD1935 pKa = 3.49NAQISSAIEE1944 pKa = 3.74DD1945 pKa = 4.07EE1946 pKa = 4.73YY1947 pKa = 11.22ILAVILANGFYY1958 pKa = 10.28DD1959 pKa = 3.83YY1960 pKa = 10.98RR1961 pKa = 11.84SSDD1964 pKa = 3.48SFNSLQSTLNSVAEE1978 pKa = 4.19NDD1980 pKa = 4.2PDD1982 pKa = 4.05LLNNNTLANYY1992 pKa = 9.73LGVGITDD1999 pKa = 3.34NVQGVDD2005 pKa = 3.31NKK2007 pKa = 11.2ADD2009 pKa = 3.54VLATFIKK2016 pKa = 10.62SVIEE2020 pKa = 3.85VLADD2024 pKa = 3.49NLSSLGGTSVTSDD2037 pKa = 3.07TLANAIQEE2045 pKa = 4.32KK2046 pKa = 10.69AEE2048 pKa = 3.86QNGIDD2053 pKa = 3.77FSLSEE2058 pKa = 4.58DD2059 pKa = 3.47ASSDD2063 pKa = 3.64SVQLVIKK2070 pKa = 9.9DD2071 pKa = 4.0PQWICQVLDD2080 pKa = 3.72LVVDD2084 pKa = 4.13GLEE2087 pKa = 4.37GLVQYY2092 pKa = 11.11DD2093 pKa = 3.67FADD2096 pKa = 3.73SFDD2099 pKa = 4.38LKK2101 pKa = 10.2TIYY2104 pKa = 10.97NLVDD2108 pKa = 3.44EE2109 pKa = 5.07VLGSLCPAASSSSSGVSSTAPSSTSGSSATSAPSGSSAPTSAATSGASGASSATGSGTPSGSGASGSGSSSATGSGSGSSGSGSSGSGSSGSGSSSATGSGSPSGSGASGASGASSATGSGSPSGASSVAPASVSGVSKK2248 pKa = 9.52GTNPTSTKK2256 pKa = 9.63TEE2258 pKa = 3.96YY2259 pKa = 10.41SHH2261 pKa = 6.75TVVTVTSCSGHH2272 pKa = 6.09KK2273 pKa = 10.31CSEE2276 pKa = 4.38TPVTTGLTTVTKK2288 pKa = 10.58DD2289 pKa = 2.98STIYY2293 pKa = 8.01TTYY2296 pKa = 10.65CPLPTEE2302 pKa = 4.33SAAPASSVAPASVTGVSKK2320 pKa = 9.64GTNPTSTKK2328 pKa = 9.63TEE2330 pKa = 3.96YY2331 pKa = 10.41SHH2333 pKa = 6.75TVVTVTSCSGNKK2345 pKa = 9.78CSEE2348 pKa = 4.39TPVTTGLTTVTKK2360 pKa = 10.58DD2361 pKa = 2.98STIYY2365 pKa = 8.01TTYY2368 pKa = 10.65CPLPTEE2374 pKa = 4.55SSAPAPVPSSKK2385 pKa = 10.5VLEE2388 pKa = 4.45SAAPASSVAPASVTGVSKK2406 pKa = 9.64GTNPTSTKK2414 pKa = 9.63TEE2416 pKa = 3.96YY2417 pKa = 10.41SHH2419 pKa = 6.75TVVTVTSCSGNKK2431 pKa = 9.78CSEE2434 pKa = 4.39TPVTTGLTTVTKK2446 pKa = 10.58DD2447 pKa = 2.98STIYY2451 pKa = 8.01TTYY2454 pKa = 10.65CPLPTEE2460 pKa = 4.55SSAPAPVPSSKK2471 pKa = 10.28VPEE2474 pKa = 4.3SAAPASSEE2482 pKa = 4.0APASKK2487 pKa = 10.65APASEE2492 pKa = 4.84GPASQAAKK2500 pKa = 10.01SSSGPAPSKK2509 pKa = 10.98APASSEE2515 pKa = 4.1APTSQAAEE2523 pKa = 4.19SSSSPVAQSSSAAAPSVNSVSEE2545 pKa = 3.93AGAIKK2550 pKa = 10.42AGISMGPLFAGAVLLLLL2567 pKa = 4.63

Molecular weight:
233.4 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|G8YHK2|G8YHK2_PICSO Piso0_003239 protein OS=Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695) OX=559304 GN=Piso0_003239 PE=4 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.35SFRR8 pKa = 11.84VKK10 pKa = 10.47QKK12 pKa = 9.98LAKK15 pKa = 9.67AQKK18 pKa = 9.21QNRR21 pKa = 11.84PLPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNKK34 pKa = 8.39IRR36 pKa = 11.84YY37 pKa = 5.83NAKK40 pKa = 8.24RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.11WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 11.12LNII51 pKa = 3.83

Molecular weight:
6.33 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8851

0

8851

4358694

40

4944

492.5

55.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.676 ± 0.023

1.186 ± 0.009

6.058 ± 0.016

6.694 ± 0.026

4.42 ± 0.018

5.381 ± 0.025

2.192 ± 0.01

6.35 ± 0.021

7.29 ± 0.027

9.321 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.95 ± 0.01

5.756 ± 0.02

4.267 ± 0.021

3.641 ± 0.018

4.573 ± 0.016

9.786 ± 0.04

5.195 ± 0.014

5.729 ± 0.015

0.999 ± 0.007

3.537 ± 0.013

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski