Clostridiales bacterium PH28_bin88

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2U857|A0A0M2U857_9FIRM Peptide ABC transporter ATP-binding protein OS=Clostridiales bacterium PH28_bin88 OX=1605376 GN=SY88_05850 PE=3 SV=1
MM1 pKa = 7.49VGVDD5 pKa = 3.24AWVDD9 pKa = 3.58STVAAPLDD17 pKa = 3.43TGAAEE22 pKa = 4.56FNPGMGMLPFAPLIPPMEE40 pKa = 4.0QFIAYY45 pKa = 7.57LTFCHH50 pKa = 6.62PPATFTATLRR60 pKa = 11.84PEE62 pKa = 3.77AA63 pKa = 4.77

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2U1T1|A0A0M2U1T1_9FIRM Uncharacterized protein OS=Clostridiales bacterium PH28_bin88 OX=1605376 GN=SY88_18720 PE=4 SV=1
MM1 pKa = 6.81KK2 pKa = 10.01QQWGRR7 pKa = 11.84VHH9 pKa = 5.11WTVALVAPQVTPQVGEE25 pKa = 4.28LLASMRR31 pKa = 11.84GEE33 pKa = 4.04MSRR36 pKa = 11.84EE37 pKa = 3.83ALQSALGLRR46 pKa = 11.84DD47 pKa = 3.15RR48 pKa = 11.84KK49 pKa = 10.15SFRR52 pKa = 11.84GRR54 pKa = 11.84YY55 pKa = 9.0LNPALVNGLIKK66 pKa = 8.64MTALEE71 pKa = 4.68KK72 pKa = 9.56PTSRR76 pKa = 11.84LQICRR81 pKa = 11.84LTDD84 pKa = 4.98KK85 pKa = 10.98GPQWQTTEE93 pKa = 3.99NQKK96 pKa = 11.24DD97 pKa = 3.56PIVRR101 pKa = 11.84YY102 pKa = 6.78QEE104 pKa = 4.7RR105 pKa = 11.84YY106 pKa = 9.21GSRR109 pKa = 11.84GDD111 pKa = 3.44SRR113 pKa = 11.84AARR116 pKa = 11.84CQSAKK121 pKa = 9.78TLPP124 pKa = 3.6

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4252

3

4255

1260418

35

2709

296.2

32.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.352 ± 0.042

1.085 ± 0.018

4.695 ± 0.029

6.903 ± 0.039

3.609 ± 0.023

8.551 ± 0.036

1.919 ± 0.017

5.759 ± 0.038

4.664 ± 0.036

10.514 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.019

3.027 ± 0.021

4.692 ± 0.025

3.519 ± 0.025

6.412 ± 0.045

4.927 ± 0.023

5.094 ± 0.027

8.545 ± 0.033

1.128 ± 0.017

2.943 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski