Entomoplasma freundtii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae; Entomoplasma

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8NRI7|A0A2K8NRI7_9MOLU Uncharacterized protein OS=Entomoplasma freundtii OX=74700 GN=EFREU_v1c04460 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.49RR3 pKa = 11.84LLTLLTAIGFTTTTAVPVVACCKK26 pKa = 10.03TNTNNSGIKK35 pKa = 10.24DD36 pKa = 3.79LGTLQIRR43 pKa = 11.84QINLPIPKK51 pKa = 8.66ATSYY55 pKa = 10.4TSKK58 pKa = 10.29QANFVISMMTQNINDD73 pKa = 4.21QINEE77 pKa = 3.91DD78 pKa = 3.91TKK80 pKa = 11.21FKK82 pKa = 11.58NNIQNQDD89 pKa = 3.72MIDD92 pKa = 3.67YY93 pKa = 10.2TDD95 pKa = 5.21DD96 pKa = 3.42FTTNIADD103 pKa = 4.87FIKK106 pKa = 10.5PDD108 pKa = 3.61EE109 pKa = 4.25TVKK112 pKa = 10.37IDD114 pKa = 3.47GSGEE118 pKa = 3.69IQINISANQNSKK130 pKa = 10.27ILTGKK135 pKa = 8.38TNATIKK141 pKa = 10.45FNPNTQLASVINNTNLGEE159 pKa = 4.2FQTEE163 pKa = 4.01PTASDD168 pKa = 3.47IIGALSALNPQALIDD183 pKa = 3.94EE184 pKa = 5.11DD185 pKa = 4.18EE186 pKa = 4.83VEE188 pKa = 4.5VLASSINLNGATVKK202 pKa = 10.91AIDD205 pKa = 4.44SNNSLYY211 pKa = 10.61EE212 pKa = 3.72GQINVTYY219 pKa = 10.4KK220 pKa = 10.86VVGKK224 pKa = 9.75KK225 pKa = 8.71QLSEE229 pKa = 4.43VITNTSLGLVNDD241 pKa = 4.24ATNEE245 pKa = 4.25TILNAINQLNPEE257 pKa = 4.56ANLKK261 pKa = 7.21TTDD264 pKa = 3.25VTIVTNSANNGATITATEE282 pKa = 3.96TSNYY286 pKa = 9.06QGSIDD291 pKa = 3.43VTYY294 pKa = 9.75TIRR297 pKa = 11.84KK298 pKa = 7.42QLSTVVTNLDD308 pKa = 4.28LGNIDD313 pKa = 3.63NNANATILAAVNAKK327 pKa = 8.47NTDD330 pKa = 3.67ANLKK334 pKa = 9.39TSDD337 pKa = 3.24VQIAVNSDD345 pKa = 3.16NNGATLTATQSSLYY359 pKa = 9.24QGSVTVTFTVKK370 pKa = 9.35TALDD374 pKa = 3.69SVVKK378 pKa = 8.74VTALGNLDD386 pKa = 3.87NADD389 pKa = 4.58DD390 pKa = 4.05DD391 pKa = 4.87TILAAVNQANSGLKK405 pKa = 9.01LTTADD410 pKa = 3.48VSIKK414 pKa = 10.93VNDD417 pKa = 4.12DD418 pKa = 3.4KK419 pKa = 11.68KK420 pKa = 11.2SATLTANPDD429 pKa = 2.95GNYY432 pKa = 9.6QGSVTVTFTIKK443 pKa = 9.04ITLDD447 pKa = 3.43SVVKK451 pKa = 9.8VTDD454 pKa = 3.78LGKK457 pKa = 11.16LNDD460 pKa = 3.94TDD462 pKa = 4.62NNTILNAVNQVNSGLNLTNNDD483 pKa = 2.61VTIAINEE490 pKa = 4.23NKK492 pKa = 10.45DD493 pKa = 3.28GATLTATSSSLYY505 pKa = 9.68KK506 pKa = 10.84GSVTVTFTVKK516 pKa = 9.35TALDD520 pKa = 3.69SVVKK524 pKa = 8.74VTALGNLDD532 pKa = 3.87NADD535 pKa = 4.58DD536 pKa = 4.05DD537 pKa = 4.87TILAAVNQANSGLKK551 pKa = 9.01LTTADD556 pKa = 3.48VSIKK560 pKa = 10.91VNDD563 pKa = 4.11DD564 pKa = 3.42NKK566 pKa = 11.01SATLTANPDD575 pKa = 2.95GNYY578 pKa = 9.6QGSVTVTFTIKK589 pKa = 10.04IALDD593 pKa = 3.61SVVKK597 pKa = 9.83VTDD600 pKa = 3.78LGKK603 pKa = 11.12LNDD606 pKa = 4.21TDD608 pKa = 4.21NSTILNAVNQVNSGLNLTNNDD629 pKa = 2.61VTIAINEE636 pKa = 4.23NKK638 pKa = 10.45DD639 pKa = 3.28GATLTATPNGNYY651 pKa = 9.81KK652 pKa = 10.78GFVNVTFIVRR662 pKa = 11.84TQLSSVVTKK671 pKa = 10.49TNLGPIIKK679 pKa = 10.06ADD681 pKa = 3.11ATTILNAVNQVNSINLNASDD701 pKa = 5.32VDD703 pKa = 3.44IQNINNSSATLTSTSSSIYY722 pKa = 9.21QGSVTVTFTIKK733 pKa = 10.04IALDD737 pKa = 3.61SVVKK741 pKa = 9.79VTDD744 pKa = 4.41LGPIDD749 pKa = 4.3KK750 pKa = 10.6ADD752 pKa = 3.56SATILAEE759 pKa = 4.17VNSVNDD765 pKa = 4.3LNLTNNDD772 pKa = 3.05VTVVTNPDD780 pKa = 3.7DD781 pKa = 4.47KK782 pKa = 11.52SATLTANPDD791 pKa = 2.95GNYY794 pKa = 9.51QGSVEE799 pKa = 4.11VTFTIKK805 pKa = 9.92ATLEE809 pKa = 4.12LVVKK813 pKa = 9.53VTDD816 pKa = 4.66LGNLDD821 pKa = 3.94PVNDD825 pKa = 3.91EE826 pKa = 4.76TILDD830 pKa = 3.72AVNAKK835 pKa = 7.9NTNVSLKK842 pKa = 10.83ASDD845 pKa = 3.34VTIAIDD851 pKa = 3.78TDD853 pKa = 3.67NKK855 pKa = 10.81SATLTSTSSSIYY867 pKa = 9.21QGSVTVTFTIKK878 pKa = 10.04IALDD882 pKa = 3.61SVVKK886 pKa = 9.83VTDD889 pKa = 3.74LGKK892 pKa = 10.86LNEE895 pKa = 4.6ANNTTILAAVNAANKK910 pKa = 9.81GLNLTTNDD918 pKa = 2.91VTIIINVTQDD928 pKa = 3.28GANLTATEE936 pKa = 3.89TSIYY940 pKa = 9.24KK941 pKa = 10.53DD942 pKa = 3.4SVSVTFNIRR951 pKa = 11.84TQLSSVVTNLNLGNIDD967 pKa = 3.83NNSEE971 pKa = 3.95ATILNAVNAANGLKK985 pKa = 9.34LTTADD990 pKa = 3.48VSIKK994 pKa = 10.91VNDD997 pKa = 4.11DD998 pKa = 3.42NKK1000 pKa = 11.01SATLTANPDD1009 pKa = 2.91GNYY1012 pKa = 9.97QGLVTVTFTIKK1023 pKa = 10.04IALDD1027 pKa = 3.78SVVKK1031 pKa = 9.95ATDD1034 pKa = 3.42LGKK1037 pKa = 11.1LNDD1040 pKa = 4.21TDD1042 pKa = 3.85SSTILAAVNQVNSGLNLTTSDD1063 pKa = 3.13VTIKK1067 pKa = 10.9VNSNSSATITATEE1080 pKa = 3.89TSNYY1084 pKa = 8.67QGSVDD1089 pKa = 3.21VTFIVRR1095 pKa = 11.84TQLSSVVSKK1104 pKa = 10.17TNLGSINKK1112 pKa = 9.36ADD1114 pKa = 3.52STTILVAVNQVNGISLTTSDD1134 pKa = 4.32VDD1136 pKa = 3.4IQIIGNDD1143 pKa = 3.72SATLTATQSSLYY1155 pKa = 9.24QGSVTVTFTIKK1166 pKa = 10.04IALDD1170 pKa = 3.61SVVKK1174 pKa = 9.83VTDD1177 pKa = 3.73LGKK1180 pKa = 11.15LNDD1183 pKa = 4.29ADD1185 pKa = 4.09NNTILAAVNQVNSGLNLTTIDD1206 pKa = 3.19VTIKK1210 pKa = 10.75VNSNSSATLTAIEE1223 pKa = 4.09TSNYY1227 pKa = 8.7QGSVDD1232 pKa = 3.21VTFIVRR1238 pKa = 11.84TQLSSVVTNLDD1249 pKa = 3.78LGDD1252 pKa = 4.31IDD1254 pKa = 5.83NLTDD1258 pKa = 5.19DD1259 pKa = 5.2IILAAVNAKK1268 pKa = 9.99NPNANLKK1275 pKa = 10.68ASDD1278 pKa = 3.83VNIEE1282 pKa = 3.79ITGDD1286 pKa = 3.38NEE1288 pKa = 3.95ATLTATSTGVYY1299 pKa = 9.02QGSVTVTFTVRR1310 pKa = 11.84TQLSSVVTNRR1320 pKa = 11.84DD1321 pKa = 2.7LGAIDD1326 pKa = 5.36NLTDD1330 pKa = 5.05DD1331 pKa = 5.21IILAAVNAKK1340 pKa = 9.99NPNANLKK1347 pKa = 10.68ASDD1350 pKa = 3.85VNIEE1354 pKa = 4.05IIGDD1358 pKa = 3.71NEE1360 pKa = 3.99ATLTATQSSIYY1371 pKa = 9.24QGSVTVTFTIKK1382 pKa = 9.21PTLDD1386 pKa = 3.77SIVKK1390 pKa = 7.81VTNLGPIDD1398 pKa = 4.2KK1399 pKa = 10.61ADD1401 pKa = 3.58STNILAAVNVKK1412 pKa = 10.34NPSANLKK1419 pKa = 9.07DD1420 pKa = 3.5TDD1422 pKa = 3.52VTIQIGGSQDD1432 pKa = 3.29KK1433 pKa = 11.11AILTATKK1440 pKa = 9.51EE1441 pKa = 4.06SAYY1444 pKa = 10.11EE1445 pKa = 3.89GTVNVTFTIKK1455 pKa = 9.94ATLDD1459 pKa = 3.7SIVKK1463 pKa = 8.11VTALGILDD1471 pKa = 3.83NTDD1474 pKa = 4.3DD1475 pKa = 3.97EE1476 pKa = 5.18TILNAVNAVNKK1487 pKa = 10.04DD1488 pKa = 3.55FQLTSDD1494 pKa = 3.47EE1495 pKa = 4.16VTIVVNNDD1503 pKa = 2.48KK1504 pKa = 10.72TGATLTANPSSIYY1517 pKa = 9.92QGSVDD1522 pKa = 3.41VTFTIKK1528 pKa = 10.17QDD1530 pKa = 3.34LDD1532 pKa = 3.81TVVVDD1537 pKa = 4.99RR1538 pKa = 11.84NLGYY1542 pKa = 10.41INNNEE1547 pKa = 3.98LSTILEE1553 pKa = 4.25AVNLQNPNVNLKK1565 pKa = 8.57EE1566 pKa = 4.05TDD1568 pKa = 3.1VTIDD1572 pKa = 3.41VKK1574 pKa = 11.53GEE1576 pKa = 3.88NNATLTATQTSNYY1589 pKa = 8.4QGSVDD1594 pKa = 3.25VTFNIRR1600 pKa = 11.84EE1601 pKa = 4.07QLDD1604 pKa = 3.73SVITNFEE1611 pKa = 4.23LGEE1614 pKa = 4.19IDD1616 pKa = 3.55NTDD1619 pKa = 3.58PATIIAAVNAKK1630 pKa = 9.98NPNANLNVSDD1640 pKa = 3.5VTIVVNSNNTFATLTATKK1658 pKa = 10.45SSIYY1662 pKa = 9.47QGSVNVVFTIKK1673 pKa = 9.8TLL1675 pKa = 3.46

Molecular weight: 176.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8NSF9|A0A2K8NSF9_9MOLU Single-stranded DNA-binding protein OS=Entomoplasma freundtii OX=74700 GN=ssb PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84KK29 pKa = 9.2VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

702

0

702

251788

30

1816

358.7

40.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.467 ± 0.077

0.594 ± 0.026

5.246 ± 0.076

6.155 ± 0.097

4.967 ± 0.085

5.915 ± 0.081

1.825 ± 0.05

8.077 ± 0.084

8.264 ± 0.075

10.435 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.041

6.291 ± 0.091

3.579 ± 0.044

4.112 ± 0.056

3.372 ± 0.054

5.668 ± 0.07

5.867 ± 0.083

6.112 ± 0.071

1.282 ± 0.037

3.547 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski