Trichoderma harzianum bipartite mycovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Curvulaviridae; Orthocurvulavirus; Trichoderma harzianum orthocurvulavirus 1

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346THI6|A0A346THI6_9VIRU Uncharacterized protein OS=Trichoderma harzianum bipartite mycovirus 1 OX=2315392 PE=4 SV=1
MM1 pKa = 7.49NAPEE5 pKa = 4.12IPGRR9 pKa = 11.84NPDD12 pKa = 3.83RR13 pKa = 11.84IRR15 pKa = 11.84RR16 pKa = 11.84SSLFNFDD23 pKa = 4.26GGLLTDD29 pKa = 3.2SHH31 pKa = 8.25GDD33 pKa = 3.31VLLNTRR39 pKa = 11.84KK40 pKa = 10.04RR41 pKa = 11.84GRR43 pKa = 11.84NQFDD47 pKa = 4.42DD48 pKa = 3.93EE49 pKa = 4.88TEE51 pKa = 4.26VTSAPSSHH59 pKa = 6.43TSGNASTRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 9.35RR71 pKa = 11.84LRR73 pKa = 11.84RR74 pKa = 11.84VRR76 pKa = 11.84MASHH80 pKa = 6.35HH81 pKa = 5.6TDD83 pKa = 2.95EE84 pKa = 4.8MSALTKK90 pKa = 10.41LFQHH94 pKa = 6.66ISILGRR100 pKa = 11.84IEE102 pKa = 4.09QYY104 pKa = 11.38NKK106 pKa = 10.49LNPDD110 pKa = 3.97LVVADD115 pKa = 4.66PDD117 pKa = 4.13PLIVEE122 pKa = 4.56YY123 pKa = 9.84TLHH126 pKa = 6.48NPPIIPNFKK135 pKa = 10.02PEE137 pKa = 4.74DD138 pKa = 3.62YY139 pKa = 10.18TFVKK143 pKa = 10.58ACTPVEE149 pKa = 4.1MAHH152 pKa = 6.73LKK154 pKa = 10.74HH155 pKa = 6.62FDD157 pKa = 3.59RR158 pKa = 11.84EE159 pKa = 4.18EE160 pKa = 4.14SVPQAEE166 pKa = 4.75YY167 pKa = 10.9LPSLQTASDD176 pKa = 3.46IVGKK180 pKa = 10.17LLSLPEE186 pKa = 3.94YY187 pKa = 10.87LFFPEE192 pKa = 5.42VDD194 pKa = 3.45DD195 pKa = 4.73LNNVRR200 pKa = 11.84YY201 pKa = 9.68IGSKK205 pKa = 10.41FPGIEE210 pKa = 3.76YY211 pKa = 10.72AMQGFKK217 pKa = 9.43TRR219 pKa = 11.84RR220 pKa = 11.84EE221 pKa = 4.04AHH223 pKa = 5.73EE224 pKa = 3.99MAMTDD229 pKa = 3.52ALSAFDD235 pKa = 4.29KK236 pKa = 10.76LLNGEE241 pKa = 4.19RR242 pKa = 11.84VKK244 pKa = 10.97PHH246 pKa = 6.54DD247 pKa = 3.56VRR249 pKa = 11.84LGGRR253 pKa = 11.84GKK255 pKa = 9.95VAEE258 pKa = 4.32MDD260 pKa = 3.35RR261 pKa = 11.84TAAEE265 pKa = 4.26AKK267 pKa = 9.31PPAVGRR273 pKa = 11.84LILMMSQRR281 pKa = 11.84DD282 pKa = 3.76LLLCGVTEE290 pKa = 4.19NLLTRR295 pKa = 11.84AYY297 pKa = 9.36TPAQFPIAVGQSWFHH312 pKa = 6.17GGATEE317 pKa = 4.45FVDD320 pKa = 3.96RR321 pKa = 11.84FYY323 pKa = 10.71PYY325 pKa = 10.19TEE327 pKa = 4.27YY328 pKa = 11.15FCFDD332 pKa = 3.16AKK334 pKa = 10.95KK335 pKa = 9.98FDD337 pKa = 3.75SSIDD341 pKa = 2.94RR342 pKa = 11.84WMVRR346 pKa = 11.84IAINILRR353 pKa = 11.84HH354 pKa = 4.61QFFEE358 pKa = 4.64GEE360 pKa = 3.95EE361 pKa = 4.23SKK363 pKa = 11.47YY364 pKa = 10.22DD365 pKa = 3.65AYY367 pKa = 8.98WTFVEE372 pKa = 5.19EE373 pKa = 4.48SLLDD377 pKa = 3.59APIYY381 pKa = 10.49RR382 pKa = 11.84DD383 pKa = 3.52DD384 pKa = 3.58GVRR387 pKa = 11.84FQKK390 pKa = 10.75HH391 pKa = 5.89CGTTSGHH398 pKa = 5.12SHH400 pKa = 5.6NTLIQSIITLLLAYY414 pKa = 7.21TTMLFLHH421 pKa = 7.07PEE423 pKa = 4.14LSVEE427 pKa = 4.37EE428 pKa = 4.15ILEE431 pKa = 4.17NMWAEE436 pKa = 4.24SLGDD440 pKa = 3.91DD441 pKa = 5.08NILGVKK447 pKa = 10.2GPLAGHH453 pKa = 5.33TVEE456 pKa = 5.9EE457 pKa = 4.48IAQVVYY463 pKa = 10.85DD464 pKa = 4.02IFRR467 pKa = 11.84VDD469 pKa = 3.0WFGKK473 pKa = 10.21KK474 pKa = 8.56SFKK477 pKa = 7.89TTCLLDD483 pKa = 4.81AITGAFQGLQFLGKK497 pKa = 8.47YY498 pKa = 9.33FRR500 pKa = 11.84IGEE503 pKa = 4.26YY504 pKa = 8.29PTADD508 pKa = 3.89KK509 pKa = 10.9LVDD512 pKa = 3.43MPIPYY517 pKa = 10.14RR518 pKa = 11.84PFKK521 pKa = 9.52EE522 pKa = 4.21TYY524 pKa = 10.02LRR526 pKa = 11.84LLYY529 pKa = 9.91PEE531 pKa = 4.91YY532 pKa = 10.53GAHH535 pKa = 6.12TPTEE539 pKa = 3.78TWLRR543 pKa = 11.84TLGNYY548 pKa = 9.75LDD550 pKa = 4.58AAGNPQAEE558 pKa = 4.49TWLSGLLTWLEE569 pKa = 4.33DD570 pKa = 3.63RR571 pKa = 11.84VEE573 pKa = 4.34TPPEE577 pKa = 3.79EE578 pKa = 3.91WPANYY583 pKa = 9.92KK584 pKa = 10.68RR585 pKa = 11.84MVSRR589 pKa = 11.84DD590 pKa = 3.52YY591 pKa = 10.61SHH593 pKa = 7.85VGIEE597 pKa = 4.03VPKK600 pKa = 9.78PVRR603 pKa = 11.84INYY606 pKa = 6.99EE607 pKa = 3.45QWRR610 pKa = 11.84DD611 pKa = 3.53LVVMSRR617 pKa = 11.84ADD619 pKa = 3.39YY620 pKa = 11.13VNTYY624 pKa = 10.22KK625 pKa = 10.74PALVKK630 pKa = 10.49QQ631 pKa = 3.73

Molecular weight: 72.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346THI6|A0A346THI6_9VIRU Uncharacterized protein OS=Trichoderma harzianum bipartite mycovirus 1 OX=2315392 PE=4 SV=1
MM1 pKa = 7.7AAQPQPSLLAQFGGANTNIDD21 pKa = 4.11FSADD25 pKa = 3.59PNRR28 pKa = 11.84LAAALPIPEE37 pKa = 4.75MGDD40 pKa = 3.26SFEE43 pKa = 3.98EE44 pKa = 4.12LQRR47 pKa = 11.84KK48 pKa = 8.21LRR50 pKa = 11.84ALAHH54 pKa = 5.4VVEE57 pKa = 4.82QGRR60 pKa = 11.84SVAVATGYY68 pKa = 10.24KK69 pKa = 9.99AAAQQEE75 pKa = 4.16RR76 pKa = 11.84DD77 pKa = 3.44PRR79 pKa = 11.84EE80 pKa = 3.88IARR83 pKa = 11.84MAMTPVEE90 pKa = 4.31IMQLDD95 pKa = 3.41AWEE98 pKa = 4.47TGAALPKK105 pKa = 9.73MDD107 pKa = 3.29WGKK110 pKa = 10.22DD111 pKa = 3.27QGEE114 pKa = 4.3PPQGDD119 pKa = 3.55ASRR122 pKa = 11.84ATALRR127 pKa = 11.84RR128 pKa = 11.84AAALNRR134 pKa = 11.84LYY136 pKa = 9.8KK137 pKa = 9.56TDD139 pKa = 3.36KK140 pKa = 10.52ATVEE144 pKa = 3.71NSTYY148 pKa = 9.98FVKK151 pKa = 10.63NYY153 pKa = 10.72SPLIPLLTAVTKK165 pKa = 10.58IIGAQRR171 pKa = 11.84DD172 pKa = 3.76LVGGMEE178 pKa = 4.86EE179 pKa = 4.19LDD181 pKa = 3.71EE182 pKa = 4.09MQMAEE187 pKa = 3.86IQTIGKK193 pKa = 9.02IVATAQTNINRR204 pKa = 11.84IQEE207 pKa = 4.06EE208 pKa = 4.35VRR210 pKa = 11.84RR211 pKa = 11.84LLRR214 pKa = 11.84DD215 pKa = 3.18INYY218 pKa = 10.18SNSVLLARR226 pKa = 11.84SHH228 pKa = 6.41ALKK231 pKa = 10.63EE232 pKa = 4.31LIPKK236 pKa = 9.18YY237 pKa = 10.0KK238 pKa = 10.14KK239 pKa = 10.63KK240 pKa = 10.38KK241 pKa = 10.55DD242 pKa = 3.66PVNDD246 pKa = 2.85ARR248 pKa = 11.84LAVGRR253 pKa = 11.84PAIGRR258 pKa = 11.84SFDD261 pKa = 3.71YY262 pKa = 10.42RR263 pKa = 11.84SKK265 pKa = 11.02AAGARR270 pKa = 11.84EE271 pKa = 3.74DD272 pKa = 3.32RR273 pKa = 11.84TNFLRR278 pKa = 11.84GTGLVAGRR286 pKa = 11.84AGPYY290 pKa = 8.86PRR292 pKa = 11.84EE293 pKa = 4.31AKK295 pKa = 9.84RR296 pKa = 11.84VRR298 pKa = 11.84TQSPNPPAEE307 pKa = 4.36EE308 pKa = 4.63GEE310 pKa = 4.31PMAQQ314 pKa = 3.28

Molecular weight: 34.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

945

314

631

472.5

53.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.206 ± 3.021

0.529 ± 0.312

5.926 ± 0.49

7.09 ± 0.049

3.915 ± 1.182

6.243 ± 0.262

2.222 ± 0.935

4.762 ± 0.009

4.868 ± 0.322

9.418 ± 0.671

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.256

3.81 ± 0.195

6.349 ± 0.2

3.704 ± 1.008

7.513 ± 0.828

4.868 ± 0.429

6.032 ± 0.552

5.714 ± 0.365

1.27 ± 0.373

3.81 ± 0.744
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski