Halioglobus japonicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Halioglobus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4451 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S9MWS2|A0A5S9MWS2_9GAMM Pesticin receptor OS=Halioglobus japonicus OX=930805 GN=fyuA_1 PE=3 SV=1
MM1 pKa = 7.41KK2 pKa = 10.38LVRR5 pKa = 11.84NTITLLTASLGAAMLATPAAAVPMXVASADD35 pKa = 3.45YY36 pKa = 10.5NGAVTFSLSNGLSVIGGVDD55 pKa = 3.16AQYY58 pKa = 7.92RR59 pKa = 11.84TPPGPPRR66 pKa = 11.84PYY68 pKa = 10.65QFNTSLNFGSITITPKK84 pKa = 9.54VTITTPEE91 pKa = 3.54IVLIPGSEE99 pKa = 4.0TCVPIFGCFTTPDD112 pKa = 3.29VSLPSQIIPLTPSISLTDD130 pKa = 3.6PVSVYY135 pKa = 10.7NYY137 pKa = 10.35SYY139 pKa = 7.63TTNGEE144 pKa = 4.05IPVGDD149 pKa = 3.65IFLTDD154 pKa = 4.18FGTPLLGEE162 pKa = 4.62ALTVDD167 pKa = 3.72DD168 pKa = 5.31LVRR171 pKa = 11.84EE172 pKa = 4.2QFQSGATSASEE183 pKa = 3.99SGTVVGDD190 pKa = 3.31LVGSYY195 pKa = 9.7EE196 pKa = 4.2YY197 pKa = 10.89EE198 pKa = 4.23GVLLPGGDD206 pKa = 3.92TIEE209 pKa = 4.92GEE211 pKa = 4.34YY212 pKa = 10.86LLNLSSPEE220 pKa = 3.8LLAEE224 pKa = 4.54LEE226 pKa = 4.19ASLLDD231 pKa = 5.03LINDD235 pKa = 3.63NTAEE239 pKa = 4.09IADD242 pKa = 3.82IALQALIASDD252 pKa = 3.9PCAGLGIGEE261 pKa = 4.93GICNDD266 pKa = 4.36VINGMDD272 pKa = 3.54SSEE275 pKa = 4.37LLVTVDD281 pKa = 4.34SIGEE285 pKa = 3.94FSSDD289 pKa = 3.1FSLQKK294 pKa = 10.62SVISVPVPATIPLFALGLALIGVNAKK320 pKa = 9.63RR321 pKa = 11.84RR322 pKa = 11.84RR323 pKa = 11.84AA324 pKa = 3.37

Molecular weight: 33.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S9QST3|A0A5S9QST3_9GAMM 50S ribosomal protein L24 OS=Halioglobus japonicus OX=930805 GN=rplX PE=3 SV=1
MM1 pKa = 7.37LWGVGIIIGLVLGLTGAGGSVFAVPLLIWIVGLEE35 pKa = 3.94PSQAIGISLGVVAIAALFGVITRR58 pKa = 11.84LRR60 pKa = 11.84SGEE63 pKa = 4.17IQWLPALVYY72 pKa = 8.85ATLGSIVAPVGQWLNQRR89 pKa = 11.84IDD91 pKa = 4.21DD92 pKa = 3.81QWLMAGFGFLVLIIALRR109 pKa = 11.84LWLQASRR116 pKa = 11.84SPQEE120 pKa = 3.72TRR122 pKa = 11.84AVRR125 pKa = 11.84ASVSAGKK132 pKa = 10.15DD133 pKa = 3.08DD134 pKa = 4.36AGAVCRR140 pKa = 11.84MINNQPFRR148 pKa = 11.84IGLPCAAGMSGGAILTGILSGLFGVGGGFIIVPTLLFLTRR188 pKa = 11.84ISIRR192 pKa = 11.84QAVATSLVVISAVGISGFSSFLLGGGNPGTGILLQVAAGSIVGMTAGVFCSRR244 pKa = 11.84FLSGPVLQKK253 pKa = 10.56VFAVMMLAIGGITVYY268 pKa = 9.73TQVLL272 pKa = 3.56

Molecular weight: 27.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4451

0

4451

1492785

29

4260

335.4

36.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.129 ± 0.041

1.102 ± 0.013

5.936 ± 0.029

6.156 ± 0.035

3.818 ± 0.024

7.692 ± 0.05

2.171 ± 0.018

5.362 ± 0.027

3.523 ± 0.025

10.376 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.019

3.499 ± 0.025

4.671 ± 0.024

4.086 ± 0.023

5.747 ± 0.029

6.393 ± 0.032

5.369 ± 0.033

7.1 ± 0.029

1.417 ± 0.014

2.954 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski