Escherichia phage IME11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; Escherichia virus IME11

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4MPX4|K4MPX4_9CAUD Portal protein OS=Escherichia phage IME11 OX=1239384 GN=IME11_7 PE=4 SV=1
MM1 pKa = 7.49ALKK4 pKa = 9.0FTFDD8 pKa = 3.76FNGITVNDD16 pKa = 4.12GVLTVSKK23 pKa = 10.41PMIDD27 pKa = 3.97GEE29 pKa = 4.56SGTITFGLLFQATSADD45 pKa = 3.81PLLKK49 pKa = 10.37SVTLSCPYY57 pKa = 10.76DD58 pKa = 3.7PFGADD63 pKa = 3.94PFTQAYY69 pKa = 9.83AYY71 pKa = 9.66IKK73 pKa = 10.31SLPDD77 pKa = 3.22YY78 pKa = 10.51EE79 pKa = 4.46SAEE82 pKa = 4.4EE83 pKa = 3.93II84 pKa = 4.27

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4MMF8|K4MMF8_9CAUD Uncharacterized protein OS=Escherichia phage IME11 OX=1239384 GN=IME11_76 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 9.51SLIMGTKK10 pKa = 9.56EE11 pKa = 3.52DD12 pKa = 3.56VSRR15 pKa = 11.84MRR17 pKa = 11.84NRR19 pKa = 11.84NVASKK24 pKa = 10.45RR25 pKa = 11.84ALSMTNEE32 pKa = 3.92QLQEE36 pKa = 4.45FIDD39 pKa = 4.23AGLKK43 pKa = 8.89QSKK46 pKa = 10.24ARR48 pKa = 11.84IVTFNHH54 pKa = 6.58PSILGNNN61 pKa = 3.73

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

22154

46

3450

243.5

27.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.703 ± 0.621

0.849 ± 0.135

5.696 ± 0.149

6.631 ± 0.286

3.756 ± 0.199

6.653 ± 0.228

1.711 ± 0.156

5.733 ± 0.257

6.405 ± 0.296

8.319 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.966 ± 0.122

5.651 ± 0.166

4.067 ± 0.169

4.297 ± 0.358

4.311 ± 0.164

6.256 ± 0.224

6.378 ± 0.28

6.748 ± 0.236

1.196 ± 0.115

3.674 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski