Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanothermobacter; Methanothermobacter thermautotrophicus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1869 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O26563|O26563_METTH Uncharacterized protein OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=MTH_463 PE=3 SV=1
MM1 pKa = 7.82SDD3 pKa = 4.05IDD5 pKa = 5.77DD6 pKa = 3.77EE7 pKa = 4.59TADD10 pKa = 3.66VLVEE14 pKa = 3.84LVNEE18 pKa = 4.16YY19 pKa = 10.43EE20 pKa = 4.19YY21 pKa = 11.48EE22 pKa = 4.03HH23 pKa = 7.81LLDD26 pKa = 4.73KK27 pKa = 11.2EE28 pKa = 4.55SVNDD32 pKa = 3.28GWKK35 pKa = 10.24RR36 pKa = 11.84FTEE39 pKa = 4.59AFYY42 pKa = 11.24DD43 pKa = 3.84DD44 pKa = 4.48NKK46 pKa = 11.31EE47 pKa = 4.14MLCDD51 pKa = 3.21TAFNYY56 pKa = 10.48VIDD59 pKa = 4.42GMPDD63 pKa = 3.06DD64 pKa = 5.65GIRR67 pKa = 11.84EE68 pKa = 4.38VFSRR72 pKa = 11.84TYY74 pKa = 10.68LDD76 pKa = 3.11NRR78 pKa = 11.84EE79 pKa = 4.12YY80 pKa = 10.4IISSFRR86 pKa = 11.84YY87 pKa = 9.8DD88 pKa = 3.49EE89 pKa = 5.4DD90 pKa = 4.35FIAYY94 pKa = 9.78FDD96 pKa = 4.51ADD98 pKa = 4.34YY99 pKa = 11.12MDD101 pKa = 6.23LDD103 pKa = 4.63LSNVDD108 pKa = 5.41LGDD111 pKa = 4.19FDD113 pKa = 5.3PLMVRR118 pKa = 11.84SWFIDD123 pKa = 3.06MLLPVALYY131 pKa = 8.36ITEE134 pKa = 4.01VMVDD138 pKa = 4.02FSYY141 pKa = 11.41DD142 pKa = 3.41LEE144 pKa = 4.88SYY146 pKa = 10.52CSVYY150 pKa = 10.56TFTVEE155 pKa = 3.92DD156 pKa = 4.35GEE158 pKa = 4.44IVDD161 pKa = 3.82VEE163 pKa = 4.28FNEE166 pKa = 4.37RR167 pKa = 11.84FIDD170 pKa = 4.03GEE172 pKa = 4.33GMMDD176 pKa = 4.05LSEE179 pKa = 4.69HH180 pKa = 6.16SLLEE184 pKa = 4.13KK185 pKa = 10.43LDD187 pKa = 4.41EE188 pKa = 4.58IKK190 pKa = 11.19NPFDD194 pKa = 3.24MNRR197 pKa = 11.84RR198 pKa = 11.84MRR200 pKa = 11.84KK201 pKa = 8.67ICSTDD206 pKa = 2.89VGRR209 pKa = 11.84EE210 pKa = 3.41EE211 pKa = 4.58ALRR214 pKa = 11.84MSYY217 pKa = 10.57FFDD220 pKa = 4.91CVDD223 pKa = 4.16CEE225 pKa = 4.47VKK227 pKa = 10.69VDD229 pKa = 3.41ILKK232 pKa = 8.72YY233 pKa = 9.53TFDD236 pKa = 4.0FLCRR240 pKa = 11.84LAPDD244 pKa = 3.71EE245 pKa = 4.09TASYY249 pKa = 9.67YY250 pKa = 9.83ISKK253 pKa = 10.51ARR255 pKa = 11.84DD256 pKa = 3.27HH257 pKa = 6.31MFEE260 pKa = 4.32IYY262 pKa = 10.27QIEE265 pKa = 3.92

Molecular weight: 31.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O27654|O27654_METTH Conserved protein OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=MTH_1617 PE=4 SV=1
MM1 pKa = 7.39SRR3 pKa = 11.84NKK5 pKa = 10.04HH6 pKa = 4.23VARR9 pKa = 11.84KK10 pKa = 9.84LRR12 pKa = 11.84MAKK15 pKa = 10.25ANRR18 pKa = 11.84QNRR21 pKa = 11.84RR22 pKa = 11.84VPAWVMVKK30 pKa = 9.15TNYY33 pKa = 9.7RR34 pKa = 11.84VRR36 pKa = 11.84SHH38 pKa = 6.66PKK40 pKa = 7.08MRR42 pKa = 11.84HH43 pKa = 3.26WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.85LKK50 pKa = 10.03VV51 pKa = 2.95

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1868

1

1869

524739

35

1787

280.8

31.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.342 ± 0.058

1.208 ± 0.032

5.914 ± 0.04

8.141 ± 0.066

3.648 ± 0.039

7.979 ± 0.055

1.874 ± 0.024

7.711 ± 0.045

4.558 ± 0.06

9.425 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.061 ± 0.027

3.309 ± 0.06

4.3 ± 0.033

1.895 ± 0.026

6.796 ± 0.062

6.14 ± 0.044

4.962 ± 0.054

7.674 ± 0.055

0.84 ± 0.018

3.224 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski