Vibrio variabilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090TFA0|A0A090TFA0_9VIBR Uncharacterized protein OS=Vibrio variabilis OX=990271 GN=JCM19239_6815 PE=4 SV=1
MM1 pKa = 7.34NKK3 pKa = 9.41VALGVATALAAASLSANASLNLNLDD28 pKa = 3.59NEE30 pKa = 4.35VDD32 pKa = 3.43GDD34 pKa = 3.92GDD36 pKa = 3.45IRR38 pKa = 11.84GLFTTVPQGDD48 pKa = 4.11NIFDD52 pKa = 3.53EE53 pKa = 4.63SMNRR57 pKa = 11.84AAHH60 pKa = 6.07NNTTATQDD68 pKa = 3.07SSTTSNGSAIQISLEE83 pKa = 4.05DD84 pKa = 3.66SDD86 pKa = 5.3YY87 pKa = 11.7SDD89 pKa = 3.44INIYY93 pKa = 10.12QSNQATTDD101 pKa = 3.08NSSTYY106 pKa = 10.72DD107 pKa = 3.3GEE109 pKa = 4.28NTVNIDD115 pKa = 3.6FDD117 pKa = 4.5SSLSTGRR124 pKa = 11.84NLDD127 pKa = 3.21NDD129 pKa = 3.43VTINQKK135 pKa = 9.85GDD137 pKa = 3.21RR138 pKa = 11.84NEE140 pKa = 3.96IYY142 pKa = 11.11VLVDD146 pKa = 3.2NEE148 pKa = 3.96APAYY152 pKa = 10.11RR153 pKa = 11.84STGNRR158 pKa = 11.84FNIDD162 pKa = 2.9QDD164 pKa = 4.09GKK166 pKa = 11.12FHH168 pKa = 7.19DD169 pKa = 4.09TTISVLDD176 pKa = 3.87GADD179 pKa = 3.31YY180 pKa = 8.9NTFNVDD186 pKa = 5.8LEE188 pKa = 4.42DD189 pKa = 3.69TNNNVDD195 pKa = 3.06ITVDD199 pKa = 3.15GRR201 pKa = 11.84SAYY204 pKa = 9.74GNSVEE209 pKa = 4.34VTSLNNSDD217 pKa = 3.82GNEE220 pKa = 3.77VSIDD224 pKa = 3.44INDD227 pKa = 3.21GHH229 pKa = 6.47MNVVDD234 pKa = 3.54VDD236 pKa = 3.85HH237 pKa = 7.21RR238 pKa = 11.84GSSDD242 pKa = 3.55DD243 pKa = 3.6NQTKK247 pKa = 10.01VEE249 pKa = 4.12VGGDD253 pKa = 3.54HH254 pKa = 6.98NDD256 pKa = 3.08VYY258 pKa = 11.89VDD260 pKa = 4.05FDD262 pKa = 4.65YY263 pKa = 11.63NSDD266 pKa = 3.8DD267 pKa = 3.66NQVLVEE273 pKa = 4.05VAGNYY278 pKa = 10.14NDD280 pKa = 3.66TDD282 pKa = 3.59IDD284 pKa = 3.63ILGGSDD290 pKa = 3.55YY291 pKa = 11.75NHH293 pKa = 5.99VQVTQLGDD301 pKa = 3.43SSVADD306 pKa = 3.33VRR308 pKa = 11.84LVNGSDD314 pKa = 3.74YY315 pKa = 11.49NDD317 pKa = 4.05MIMVTQTTNDD327 pKa = 3.31YY328 pKa = 11.35ANVYY332 pKa = 9.79GSNADD337 pKa = 3.77YY338 pKa = 11.51NSVTVTQNN346 pKa = 2.55

Molecular weight: 37.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090SKD9|A0A090SKD9_9VIBR Probable transmembrane protein OS=Vibrio variabilis OX=990271 GN=JCM19239_6793 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.49RR13 pKa = 11.84KK14 pKa = 7.97RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.44NGRR29 pKa = 11.84KK30 pKa = 9.39VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84LSKK45 pKa = 10.83

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7941

0

7941

1574166

37

2388

198.2

22.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.566 ± 0.037

1.044 ± 0.012

5.568 ± 0.034

6.363 ± 0.03

4.129 ± 0.023

6.897 ± 0.031

2.191 ± 0.016

6.165 ± 0.027

5.157 ± 0.023

10.108 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.018

4.152 ± 0.02

3.834 ± 0.019

4.301 ± 0.024

4.517 ± 0.026

6.884 ± 0.028

5.599 ± 0.023

7.257 ± 0.028

1.292 ± 0.015

3.062 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski