Proteome-pI 2.0 - Proteome Isoelectric Point Database

Rhodobacteraceae bacterium EhC02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3813 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1B6YPK8|A0A1B6YPK8_9RHOB Proline--tRNA ligase OS=Rhodobacteraceae bacterium EhC02 OX=1849170 GN=proS PE=3 SV=1

MM1 pKa = 8.33DD2 pKa = 6.23DD3 pKa = 5.03NIDD6 pKa = 3.99LEE8 pKa = 4.67ALDD11 pKa = 5.67GYY13 pKa = 10.87LSSDD17 pKa = 4.15DD18 pKa = 4.31SPEE21 pKa = 3.87NCMMLSDD28 pKa = 4.94LDD30 pKa = 4.11GFIHH34 pKa = 7.28GVICSPVMIHH44 pKa = 6.23SDD46 pKa = 2.82EE47 pKa = 4.17WLPVALGGDD56 pKa = 3.79PGEE59 pKa = 4.31VPVWVLNDD67 pKa = 3.07ITDD70 pKa = 4.05LYY72 pKa = 10.48MGIAQGLVADD82 pKa = 4.65PPVVEE87 pKa = 4.81PIFWQAHH94 pKa = 4.87EE95 pKa = 4.2GHH97 pKa = 6.79VIAMDD102 pKa = 3.12WCEE105 pKa = 4.26GFMQAVALRR114 pKa = 11.84PKK116 pKa = 9.41EE117 pKa = 3.95WLRR120 pKa = 11.84LTEE123 pKa = 4.36SGTDD127 pKa = 3.3GHH129 pKa = 6.78LVNPIVAHH137 pKa = 6.72LLDD140 pKa = 5.24DD141 pKa = 4.58NGNSLLGIPQEE152 pKa = 4.31EE153 pKa = 4.49LDD155 pKa = 3.84QVLAEE160 pKa = 4.59AADD163 pKa = 4.91AIPDD167 pKa = 3.51AVAEE171 pKa = 4.1IFSYY175 pKa = 9.48WRR177 pKa = 11.84QAA179 pKa = 2.91

Molecular weight: 19.59 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1B6YQP4|A0A1B6YQP4_9RHOB Transcriptional regulator OS=Rhodobacteraceae bacterium EhC02 OX=1849170 GN=A8B83_11295 PE=3 SV=1

MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.03ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.19 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3813	0	3813	1208368	41	3252	316.9	34.29

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
12.895 ± 0.062	0.875 ± 0.013	6.099 ± 0.039	5.295 ± 0.038	3.611 ± 0.024	8.862 ± 0.048	2.109 ± 0.02	5.209 ± 0.027	2.794 ± 0.034	10.259 ± 0.042

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.999 ± 0.023	2.455 ± 0.025	5.176 ± 0.031	3.34 ± 0.024	6.975 ± 0.04	4.866 ± 0.026	5.467 ± 0.03	7.234 ± 0.035	1.388 ± 0.017	2.091 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski