Ferruginibacter sp. BO-59

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Ferruginibacter; unclassified Ferruginibacter

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M9NJX1|A0A3M9NJX1_9BACT 3-isopropylmalate dehydratase small subunit OS=Ferruginibacter sp. BO-59 OX=2294116 GN=leuD PE=3 SV=1
MM1 pKa = 7.66SDD3 pKa = 4.69DD4 pKa = 4.99FISEE8 pKa = 4.05NDD10 pKa = 3.36VLSFLLRR17 pKa = 11.84AFGWYY22 pKa = 10.47LFFNFFFSPEE32 pKa = 3.8AGSILFSDD40 pKa = 3.89SCFLLSAFGFTLSAFNHH57 pKa = 5.36FTSSLFCSSPVAATTT72 pKa = 3.54

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M9N9Q7|A0A3M9N9Q7_9BACT Uncharacterized protein OS=Ferruginibacter sp. BO-59 OX=2294116 GN=EFY79_14900 PE=4 SV=1
MM1 pKa = 7.4NKK3 pKa = 9.98LAIRR7 pKa = 11.84KK8 pKa = 8.48NVLSNLLKK16 pKa = 10.89GKK18 pKa = 10.31APRR21 pKa = 11.84KK22 pKa = 9.37NVLPKK27 pKa = 10.33LLKK30 pKa = 10.44GKK32 pKa = 10.21APRR35 pKa = 11.84KK36 pKa = 9.55IILSNLLKK44 pKa = 10.77GKK46 pKa = 10.24APRR49 pKa = 11.84KK50 pKa = 9.55IILSNLLKK58 pKa = 10.86GKK60 pKa = 10.53APGKK64 pKa = 10.06NVLSNLLKK72 pKa = 10.6GKK74 pKa = 9.59RR75 pKa = 11.84LRR77 pKa = 11.84KK78 pKa = 9.46NVLPLLFIVFLKK90 pKa = 9.46MQLVV94 pKa = 3.6

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4167

0

4167

1453408

26

2398

348.8

39.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.862 ± 0.035

0.861 ± 0.011

5.322 ± 0.026

5.751 ± 0.044

5.287 ± 0.031

6.656 ± 0.036

1.874 ± 0.016

7.719 ± 0.035

7.617 ± 0.036

9.026 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.017

6.042 ± 0.036

3.949 ± 0.026

3.538 ± 0.019

3.669 ± 0.023

6.741 ± 0.036

5.432 ± 0.037

6.031 ± 0.028

1.299 ± 0.016

4.012 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski