Novosphingobium sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519QZG0|A0A519QZG0_9SPHN Nuclear transport factor 2 family protein OS=Novosphingobium sp. OX=1874826 GN=EOO76_00640 PE=4 SV=1
MM1 pKa = 7.2VAITFIAADD10 pKa = 3.7GAEE13 pKa = 4.09RR14 pKa = 11.84TIDD17 pKa = 3.46ASEE20 pKa = 4.61DD21 pKa = 3.27LSLMEE26 pKa = 4.26NAVGNGIEE34 pKa = 4.68AIEE37 pKa = 4.19AVCSGNAYY45 pKa = 10.57CGTCRR50 pKa = 11.84VYY52 pKa = 11.35VDD54 pKa = 5.05ADD56 pKa = 3.23WQGRR60 pKa = 11.84LGEE63 pKa = 4.73AGDD66 pKa = 4.01IEE68 pKa = 5.19LPMIEE73 pKa = 4.41ASGDD77 pKa = 3.37EE78 pKa = 4.47TPGVRR83 pKa = 11.84LSCQIVVTPDD93 pKa = 3.11LDD95 pKa = 3.77GLVVRR100 pKa = 11.84TPASQGG106 pKa = 3.19

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A519QNU0|A0A519QNU0_9SPHN Alpha/beta hydrolase OS=Novosphingobium sp. OX=1874826 GN=EOO76_13265 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.45GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4332

0

4332

1345091

21

4287

310.5

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.223 ± 0.055

0.791 ± 0.012

5.877 ± 0.03

5.67 ± 0.031

3.616 ± 0.021

8.938 ± 0.032

1.99 ± 0.02

4.842 ± 0.023

3.054 ± 0.03

9.912 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.02

2.591 ± 0.031

5.337 ± 0.025

3.208 ± 0.022

7.16 ± 0.041

5.195 ± 0.028

5.365 ± 0.045

7.253 ± 0.027

1.445 ± 0.016

2.236 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski