Mycobacterium phage QueenHazel

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Chebruvirinae; Brujitavirus; unclassified Brujitavirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TW72|A0A5J6TW72_9CAUD 4Fe-4S Wbl-type domain-containing protein OS=Mycobacterium phage QueenHazel OX=2599875 GN=40 PE=3 SV=1
MM1 pKa = 7.66TDD3 pKa = 4.76CLLCDD8 pKa = 4.18HH9 pKa = 7.19PRR11 pKa = 11.84SSHH14 pKa = 6.49APQCRR19 pKa = 11.84VRR21 pKa = 11.84MGVNRR26 pKa = 11.84DD27 pKa = 3.32DD28 pKa = 3.66MNTYY32 pKa = 8.02TICLCPGFEE41 pKa = 4.37GAEE44 pKa = 4.07EE45 pKa = 4.27DD46 pKa = 5.56DD47 pKa = 4.12EE48 pKa = 4.71LL49 pKa = 6.16

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TYR7|A0A5J6TYR7_9CAUD Uncharacterized protein OS=Mycobacterium phage QueenHazel OX=2599875 GN=39 PE=4 SV=1
MM1 pKa = 6.89VNRR4 pKa = 11.84WRR6 pKa = 11.84TWQFAQSLSRR16 pKa = 11.84RR17 pKa = 11.84TVEE20 pKa = 3.71EE21 pKa = 3.55RR22 pKa = 11.84VATVRR27 pKa = 11.84RR28 pKa = 11.84MAEE31 pKa = 3.56WCGVSPEE38 pKa = 4.09FAQVEE43 pKa = 4.5QIVEE47 pKa = 3.97WLAEE51 pKa = 4.18GGTWSARR58 pKa = 11.84TRR60 pKa = 11.84WTYY63 pKa = 11.34YY64 pKa = 10.31GALSAWFLWLQQQGYY79 pKa = 8.47RR80 pKa = 11.84QDD82 pKa = 3.5NPMVMIGRR90 pKa = 11.84PKK92 pKa = 10.09RR93 pKa = 11.84PKK95 pKa = 9.75SVPRR99 pKa = 11.84PVSNLDD105 pKa = 3.3MQRR108 pKa = 11.84LLAVRR113 pKa = 11.84AHH115 pKa = 6.47KK116 pKa = 9.25RR117 pKa = 11.84TKK119 pKa = 11.33AMILLAAFQGLRR131 pKa = 11.84VHH133 pKa = 7.38EE134 pKa = 4.11IAQIKK139 pKa = 10.05GEE141 pKa = 4.07HH142 pKa = 6.8LDD144 pKa = 4.12LIEE147 pKa = 4.13RR148 pKa = 11.84TMTVTGKK155 pKa = 11.18GNITATLPLHH165 pKa = 5.86HH166 pKa = 6.81RR167 pKa = 11.84VVEE170 pKa = 4.17MAYY173 pKa = 9.4QMPRR177 pKa = 11.84KK178 pKa = 9.17GHH180 pKa = 6.4WFPGPDD186 pKa = 2.81RR187 pKa = 11.84GHH189 pKa = 5.51QRR191 pKa = 11.84RR192 pKa = 11.84EE193 pKa = 4.31SVSGTIKK200 pKa = 10.28EE201 pKa = 4.01AMIRR205 pKa = 11.84AGVLGSAHH213 pKa = 7.05CLRR216 pKa = 11.84HH217 pKa = 4.94WFGTALLEE225 pKa = 4.69AGVDD229 pKa = 3.56LRR231 pKa = 11.84TVQEE235 pKa = 4.11LMRR238 pKa = 11.84HH239 pKa = 4.7QSLTSTEE246 pKa = 3.62IYY248 pKa = 10.24TRR250 pKa = 11.84VTDD253 pKa = 3.51RR254 pKa = 11.84RR255 pKa = 11.84RR256 pKa = 11.84AEE258 pKa = 4.57GIQRR262 pKa = 11.84LDD264 pKa = 3.27PFGVARR270 pKa = 11.84QGHH273 pKa = 5.86KK274 pKa = 10.41AADD277 pKa = 3.78IIGEE281 pKa = 4.19MIADD285 pKa = 4.18EE286 pKa = 4.42VAGSGASPAAA296 pKa = 3.84

Molecular weight: 33.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15516

40

1273

212.5

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.904 ± 0.504

1.051 ± 0.14

6.864 ± 0.232

5.936 ± 0.282

2.952 ± 0.144

8.875 ± 0.493

2.133 ± 0.239

4.331 ± 0.18

3.229 ± 0.224

7.856 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.129

3.126 ± 0.202

6.219 ± 0.2

3.828 ± 0.204

7.064 ± 0.396

5.079 ± 0.197

6.013 ± 0.252

6.806 ± 0.254

2.153 ± 0.177

2.468 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski