Bacteroidetes bacterium UKL13-3

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; unclassified Bacteroidetes

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2576 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142L0K5|A0A142L0K5_9BACT Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta OS=Bacteroidetes bacterium UKL13-3 OX=1690483 GN=accD PE=3 SV=1
MM1 pKa = 7.48QDD3 pKa = 3.92LEE5 pKa = 4.22PHH7 pKa = 5.81FRR9 pKa = 11.84WRR11 pKa = 11.84DD12 pKa = 3.42LYY14 pKa = 11.01RR15 pKa = 11.84SEE17 pKa = 5.85DD18 pKa = 3.46DD19 pKa = 3.84HH20 pKa = 7.97LSPFYY25 pKa = 10.38EE26 pKa = 3.87RR27 pKa = 11.84EE28 pKa = 3.92YY29 pKa = 11.5SEE31 pKa = 5.65FEE33 pKa = 4.19YY34 pKa = 10.97TNQVYY39 pKa = 10.53NYY41 pKa = 9.38LLHH44 pKa = 6.14PQWDD48 pKa = 4.15EE49 pKa = 4.14FGSPTLYY56 pKa = 10.41IKK58 pKa = 10.49ILFADD63 pKa = 3.94YY64 pKa = 11.22ADD66 pKa = 4.43GYY68 pKa = 11.28AIIEE72 pKa = 4.72MIGEE76 pKa = 4.03WNDD79 pKa = 3.11AVTNDD84 pKa = 2.96IMYY87 pKa = 10.27LKK89 pKa = 10.13RR90 pKa = 11.84DD91 pKa = 3.65VVEE94 pKa = 4.4VLAEE98 pKa = 3.73NGINKK103 pKa = 9.9FILIGEE109 pKa = 4.29NVLNFHH115 pKa = 6.69TNDD118 pKa = 3.18DD119 pKa = 3.92SYY121 pKa = 11.32YY122 pKa = 9.64EE123 pKa = 3.88EE124 pKa = 4.22WFEE127 pKa = 4.79DD128 pKa = 5.03VEE130 pKa = 4.8DD131 pKa = 3.8GWIAALNFQEE141 pKa = 5.66HH142 pKa = 5.54VLQEE146 pKa = 4.16FRR148 pKa = 11.84DD149 pKa = 4.11GNLDD153 pKa = 3.37YY154 pKa = 11.06YY155 pKa = 10.58INFGGEE161 pKa = 4.39LDD163 pKa = 3.58NMIWRR168 pKa = 11.84KK169 pKa = 9.55LSPMQVFNQVKK180 pKa = 10.15SILEE184 pKa = 3.89FRR186 pKa = 11.84LSS188 pKa = 3.24

Molecular weight: 22.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142L1B9|A0A142L1B9_9BACT Chromosome segregation protein SMC OS=Bacteroidetes bacterium UKL13-3 OX=1690483 GN=AEM51_02300 PE=4 SV=1
MM1 pKa = 8.02PLRR4 pKa = 11.84TFQPSVRR11 pKa = 11.84KK12 pKa = 9.55RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.51HH17 pKa = 3.96GFRR20 pKa = 11.84EE21 pKa = 4.21RR22 pKa = 11.84MSTAAGRR29 pKa = 11.84KK30 pKa = 8.89VIAARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 8.72KK42 pKa = 10.08LTVSSEE48 pKa = 3.88KK49 pKa = 10.0RR50 pKa = 11.84HH51 pKa = 5.63KK52 pKa = 10.4KK53 pKa = 9.77

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2576

0

2576

874788

38

7908

339.6

38.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.827 ± 0.056

0.958 ± 0.018

5.048 ± 0.046

5.794 ± 0.084

5.04 ± 0.04

6.511 ± 0.071

1.91 ± 0.027

7.761 ± 0.04

7.219 ± 0.092

9.213 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.034

6.057 ± 0.069

3.557 ± 0.032

3.748 ± 0.035

3.685 ± 0.029

6.525 ± 0.07

6.246 ± 0.131

6.356 ± 0.041

1.049 ± 0.015

4.086 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski