Mannheimia phage vB_MhS_535AP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3LSX4|A0A0M3LSX4_9CAUD Uncharacterized protein OS=Mannheimia phage vB_MhS_535AP2 OX=1572743 GN=535AP2_50 PE=4 SV=1
MM1 pKa = 7.68NEE3 pKa = 3.68EE4 pKa = 4.84KK5 pKa = 10.81YY6 pKa = 11.02FSVDD10 pKa = 2.99VSDD13 pKa = 3.64EE14 pKa = 4.11THH16 pKa = 6.84VIRR19 pKa = 11.84LHH21 pKa = 5.55EE22 pKa = 4.34TLEE25 pKa = 4.09QAKK28 pKa = 9.83QSCLNGATEE37 pKa = 4.64AYY39 pKa = 9.52EE40 pKa = 4.2FAGDD44 pKa = 3.78MDD46 pKa = 3.97DD47 pKa = 4.09HH48 pKa = 6.86EE49 pKa = 5.61SYY51 pKa = 11.0EE52 pKa = 4.33SYY54 pKa = 10.55EE55 pKa = 4.7AYY57 pKa = 10.33DD58 pKa = 3.71LPYY61 pKa = 10.7AVYY64 pKa = 10.22GVVLGRR70 pKa = 11.84AKK72 pKa = 10.72SDD74 pKa = 3.03IRR76 pKa = 11.84PLTDD80 pKa = 2.99EE81 pKa = 4.38EE82 pKa = 4.39RR83 pKa = 11.84ASEE86 pKa = 4.11LFGEE90 pKa = 4.72AEE92 pKa = 4.12QVIEE96 pKa = 4.72PPTLLEE102 pKa = 4.12NNGWISIEE110 pKa = 4.33DD111 pKa = 3.78KK112 pKa = 10.91LPPIEE117 pKa = 4.18TDD119 pKa = 3.54VLGLCDD125 pKa = 3.61ISGMQLILIVSRR137 pKa = 11.84EE138 pKa = 3.88LADD141 pKa = 3.7NEE143 pKa = 4.5WYY145 pKa = 10.22FLSVNQYY152 pKa = 11.14GLDD155 pKa = 3.77DD156 pKa = 4.54DD157 pKa = 5.49VIAVTHH163 pKa = 6.15WQPLPEE169 pKa = 4.46PPKK172 pKa = 10.36EE173 pKa = 4.13EE174 pKa = 4.18KK175 pKa = 10.71

Molecular weight: 19.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3LSJ6|A0A0M3LSJ6_9CAUD Uncharacterized protein OS=Mannheimia phage vB_MhS_535AP2 OX=1572743 GN=535AP2_09 PE=4 SV=1
MM1 pKa = 7.45GNKK4 pKa = 8.25KK5 pKa = 8.69TSNTANVRR13 pKa = 11.84GFVLYY18 pKa = 10.27KK19 pKa = 9.96SHH21 pKa = 7.12HH22 pKa = 6.41LGISSVFYY30 pKa = 7.79PTNPEE35 pKa = 4.05TIAKK39 pKa = 9.24LCNIYY44 pKa = 10.32FPSKK48 pKa = 10.74QKK50 pKa = 10.53GSCYY54 pKa = 10.73APIFNSGG61 pKa = 2.84

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15084

38

2241

190.9

21.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.174 ± 0.624

1.008 ± 0.189

5.237 ± 0.196

7.2 ± 0.403

3.812 ± 0.259

6.358 ± 0.267

1.604 ± 0.157

7.001 ± 0.214

7.246 ± 0.391

8.307 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.213

5.841 ± 0.327

3.116 ± 0.227

4.946 ± 0.27

4.879 ± 0.228

6.431 ± 0.29

5.88 ± 0.603

5.993 ± 0.238

1.352 ± 0.118

3.381 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski