Cronobacter phage GW1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Cronobacter virus GW1

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7N8Q8|A0A3S7N8Q8_9CAUD Uncharacterized protein OS=Cronobacter phage GW1 OX=2200756 GN=GW1_00038 PE=4 SV=1
MM1 pKa = 7.39SLLKK5 pKa = 10.76RR6 pKa = 11.84FDD8 pKa = 4.66DD9 pKa = 5.65LMSHH13 pKa = 7.29LNGEE17 pKa = 4.51DD18 pKa = 3.15NSLAEE23 pKa = 4.54EE24 pKa = 3.97FRR26 pKa = 11.84EE27 pKa = 4.19ALSEE31 pKa = 4.09AVEE34 pKa = 4.35DD35 pKa = 4.43LQFLDD40 pKa = 3.71ALRR43 pKa = 11.84AAGVDD48 pKa = 2.8NWEE51 pKa = 4.68GYY53 pKa = 10.02EE54 pKa = 4.1CAQEE58 pKa = 3.9LMEE61 pKa = 4.98YY62 pKa = 10.45EE63 pKa = 4.5DD64 pKa = 5.88DD65 pKa = 4.0EE66 pKa = 4.64

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7N8N3|A0A3S7N8N3_9CAUD Uncharacterized protein OS=Cronobacter phage GW1 OX=2200756 GN=GW1_00017 PE=4 SV=1
MM1 pKa = 7.42GWLLLVIGYY10 pKa = 9.16GLILAVVTKK19 pKa = 10.36DD20 pKa = 2.95IIKK23 pKa = 10.1ARR25 pKa = 11.84KK26 pKa = 8.36VYY28 pKa = 9.89RR29 pKa = 11.84FQYY32 pKa = 10.09VSLGRR37 pKa = 11.84WTVRR41 pKa = 11.84QPNGRR46 pKa = 11.84FMRR49 pKa = 11.84NLANVWDD56 pKa = 4.02IATLGSKK63 pKa = 10.07LL64 pKa = 3.58

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12028

37

1217

245.5

27.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.037 ± 0.42

0.873 ± 0.175

6.219 ± 0.275

6.759 ± 0.403

3.708 ± 0.19

7.998 ± 0.355

1.696 ± 0.199

4.706 ± 0.148

6.46 ± 0.35

8.156 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.133

4.182 ± 0.221

3.799 ± 0.26

4.29 ± 0.41

5.321 ± 0.198

6.194 ± 0.257

5.72 ± 0.323

7.092 ± 0.311

1.497 ± 0.178

3.533 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski