Strawberry mild yellow edge-associated virus (SMYEaV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|Q00848|TGB3_SMYEA Movement protein TGBp3 OS=Strawberry mild yellow edge-associated virus OX=12187 GN=ORF4 PE=3 SV=1
MM1 pKa = 8.47VEE3 pKa = 3.87FTKK6 pKa = 10.79RR7 pKa = 11.84LLLEE11 pKa = 4.44RR12 pKa = 11.84NFEE15 pKa = 4.1RR16 pKa = 11.84TNRR19 pKa = 11.84PITGPIVVHH28 pKa = 6.33AVAGAGKK35 pKa = 10.02SSVINTVSLTFQLICWTTLPEE56 pKa = 4.06EE57 pKa = 4.33KK58 pKa = 10.64ASFNCLHH65 pKa = 6.46LRR67 pKa = 11.84HH68 pKa = 6.62LDD70 pKa = 3.59GPAFPGAFVDD80 pKa = 5.08EE81 pKa = 4.46YY82 pKa = 11.61QLADD86 pKa = 3.54TDD88 pKa = 4.0LSEE91 pKa = 4.38AAFLFGDD98 pKa = 4.15PLQYY102 pKa = 10.1PGPAAQVPHH111 pKa = 6.03FVKK114 pKa = 10.61LFSHH118 pKa = 6.69RR119 pKa = 11.84CGLNSASLIRR129 pKa = 11.84EE130 pKa = 3.9LGIAFEE136 pKa = 4.12ASKK139 pKa = 10.81LDD141 pKa = 3.92SVQHH145 pKa = 6.45LDD147 pKa = 4.18PYY149 pKa = 11.25SSDD152 pKa = 3.6PEE154 pKa = 4.16GTILAFEE161 pKa = 4.95PEE163 pKa = 4.32VQAALASHH171 pKa = 6.61SLDD174 pKa = 3.66FLCLDD179 pKa = 4.26EE180 pKa = 5.84FRR182 pKa = 11.84GKK184 pKa = 9.04QWPVCTLYY192 pKa = 11.21VSTKK196 pKa = 10.11NLCDD200 pKa = 3.92LDD202 pKa = 4.25RR203 pKa = 11.84PSVYY207 pKa = 10.35VALTRR212 pKa = 11.84HH213 pKa = 5.36YY214 pKa = 10.41EE215 pKa = 3.84RR216 pKa = 11.84LLIMSFDD223 pKa = 3.99AADD226 pKa = 3.57TSAA229 pKa = 4.49

Molecular weight:
25.39 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q88468|Q88468_SMYEA 11K protein OS=Strawberry mild yellow edge-associated virus OX=12187 PE=4 SV=1
MM1 pKa = 7.29TLALHH6 pKa = 6.68PLPNRR11 pKa = 11.84SVLHH15 pKa = 6.12PSAARR20 pKa = 11.84ACKK23 pKa = 10.16SPPSFEE29 pKa = 3.81ITALSASSVCSTPQASGTKK48 pKa = 9.41QSGTTVPLAIGAISSLHH65 pKa = 6.02RR66 pKa = 11.84RR67 pKa = 11.84PSSLPLISSMVCSIQPAKK85 pKa = 10.37KK86 pKa = 10.02SRR88 pKa = 11.84FGVSPPPRR96 pKa = 11.84RR97 pKa = 11.84FMPQPRR103 pKa = 11.84IRR105 pKa = 11.84MM106 pKa = 3.79

Molecular weight:
11.23 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2083

75

1323

347.2

38.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.785 ± 0.787

2.064 ± 0.496

5.713 ± 0.856

4.897 ± 1.034

4.273 ± 0.363

5.233 ± 0.425

2.784 ± 0.26

4.609 ± 0.477

5.473 ± 1.625

9.65 ± 0.681

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.488 ± 0.194

4.033 ± 0.593

7.489 ± 1.861

3.505 ± 0.323

5.377 ± 0.333

7.249 ± 1.519

6.097 ± 0.164

6.673 ± 0.18

1.344 ± 0.295

3.265 ± 0.509

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski