Mesonia phycicola

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Mesonia

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6C3A9|A0A1M6C3A9_9FLAO Glucoamylase (Glucan-1 4-alpha-glucosidase) GH15 family OS=Mesonia phycicola OX=579105 GN=SAMN04488096_102334 PE=4 SV=1
MM1 pKa = 7.58IKK3 pKa = 10.31GKK5 pKa = 10.57KK6 pKa = 9.65FFIGLALASLGVACSSDD23 pKa = 3.65DD24 pKa = 4.63DD25 pKa = 4.29FDD27 pKa = 4.7RR28 pKa = 11.84GNWVEE33 pKa = 4.23SSVFDD38 pKa = 3.7GVPRR42 pKa = 11.84SSAAAFTIDD51 pKa = 2.93NLGYY55 pKa = 9.95MGTGYY60 pKa = 10.89DD61 pKa = 3.7GDD63 pKa = 5.42DD64 pKa = 3.64YY65 pKa = 11.8LSDD68 pKa = 3.12FWEE71 pKa = 4.23YY72 pKa = 11.1NIEE75 pKa = 3.67GDD77 pKa = 3.85YY78 pKa = 10.02WVQKK82 pKa = 11.07ADD84 pKa = 3.89FPSTPRR90 pKa = 11.84SAASAFTIDD99 pKa = 3.09GKK101 pKa = 11.43GYY103 pKa = 10.56LGLGYY108 pKa = 10.68DD109 pKa = 5.32GDD111 pKa = 6.11DD112 pKa = 3.49EE113 pKa = 5.93LQDD116 pKa = 3.4FWEE119 pKa = 4.56YY120 pKa = 11.59NPATNTWDD128 pKa = 3.62SIADD132 pKa = 3.48FGGGVRR138 pKa = 11.84RR139 pKa = 11.84SATGFAINGTGYY151 pKa = 10.91VGTGYY156 pKa = 10.91DD157 pKa = 3.99GDD159 pKa = 4.53NDD161 pKa = 5.42LKK163 pKa = 11.27DD164 pKa = 3.62FWKK167 pKa = 10.8YY168 pKa = 10.95NPSTDD173 pKa = 2.43QWTEE177 pKa = 3.55MVGFGGGKK185 pKa = 9.59RR186 pKa = 11.84RR187 pKa = 11.84DD188 pKa = 3.16AAYY191 pKa = 10.04FILNDD196 pKa = 3.68LVYY199 pKa = 10.57FGTGVTNGVYY209 pKa = 8.74NTDD212 pKa = 3.67FWQFDD217 pKa = 3.68PSSEE221 pKa = 3.68VWTRR225 pKa = 11.84LNDD228 pKa = 3.7LDD230 pKa = 5.25EE231 pKa = 4.72EE232 pKa = 4.5DD233 pKa = 4.05SYY235 pKa = 12.21SVVRR239 pKa = 11.84SNAVGFSQAGYY250 pKa = 10.15GYY252 pKa = 10.37FATGYY257 pKa = 10.55NGGALDD263 pKa = 4.89TIWEE267 pKa = 4.14YY268 pKa = 11.71DD269 pKa = 3.49PTNDD273 pKa = 3.16DD274 pKa = 3.41WEE276 pKa = 5.22KK277 pKa = 9.5ITEE280 pKa = 4.24FEE282 pKa = 3.81ATARR286 pKa = 11.84QDD288 pKa = 3.21AVSFSTATRR297 pKa = 11.84AFVLLGRR304 pKa = 11.84TGSLYY309 pKa = 10.84LDD311 pKa = 4.19DD312 pKa = 4.76NYY314 pKa = 11.04EE315 pKa = 3.94VFTQDD320 pKa = 4.3EE321 pKa = 4.5YY322 pKa = 11.78DD323 pKa = 3.83EE324 pKa = 4.73EE325 pKa = 4.56DD326 pKa = 3.13

Molecular weight: 36.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6C0F6|A0A1M6C0F6_9FLAO CRP/FNR family transcriptional regulator anaerobic regulatory protein OS=Mesonia phycicola OX=579105 GN=SAMN04488096_102309 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.05GFRR19 pKa = 11.84EE20 pKa = 4.13RR21 pKa = 11.84MSSVNGRR28 pKa = 11.84KK29 pKa = 9.17VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2974

0

2974

992302

40

3340

333.7

37.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.095 ± 0.038

0.715 ± 0.013

5.351 ± 0.042

7.202 ± 0.046

5.259 ± 0.042

5.988 ± 0.046

1.692 ± 0.021

8.043 ± 0.046

8.156 ± 0.071

9.391 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.021

6.49 ± 0.047

3.204 ± 0.023

3.653 ± 0.026

3.085 ± 0.028

6.568 ± 0.043

5.78 ± 0.05

6.077 ± 0.034

0.998 ± 0.016

4.272 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski