Fragaria chiloensis latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5VI74|Q5VI74_9BROM RNA-directed RNA polymerase 2a OS=Fragaria chiloensis latent virus OX=255238 PE=4 SV=1
MM1 pKa = 7.69FCPKK5 pKa = 9.76ILSFVPSSEE14 pKa = 3.72PWVGSEE20 pKa = 4.86SLDD23 pKa = 3.46ASGDD27 pKa = 3.35IPTIDD32 pKa = 3.87DD33 pKa = 3.9WYY35 pKa = 11.3LLTNTVLAEE44 pKa = 3.74RR45 pKa = 11.84LVIEE49 pKa = 5.27RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84QLEE55 pKa = 4.25KK56 pKa = 11.08VVGQYY61 pKa = 7.72KK62 pKa = 8.97TLLHH66 pKa = 5.71FVGWCYY72 pKa = 10.91LEE74 pKa = 3.91QVVFKK79 pKa = 9.46TSKK82 pKa = 10.74YY83 pKa = 9.34KK84 pKa = 11.23GEE86 pKa = 4.21FNTFPFLVEE95 pKa = 3.73RR96 pKa = 11.84FFAAFDD102 pKa = 3.92LEE104 pKa = 4.31APQYY108 pKa = 10.47EE109 pKa = 4.75VLPMQSDD116 pKa = 4.15CLSDD120 pKa = 4.41FFDD123 pKa = 3.95VGCVDD128 pKa = 4.43LPLPIEE134 pKa = 4.61EE135 pKa = 4.81DD136 pKa = 4.06TISLPSDD143 pKa = 3.13FHH145 pKa = 8.61RR146 pKa = 11.84SISSLSYY153 pKa = 9.01PSCGSSSMEE162 pKa = 4.0SGRR165 pKa = 11.84TSSGGPEE172 pKa = 4.01STSSDD177 pKa = 3.7VCHH180 pKa = 7.04EE181 pKa = 4.45IYY183 pKa = 10.63NAGPVDD189 pKa = 4.25PMPQCSRR196 pKa = 11.84RR197 pKa = 11.84WSDD200 pKa = 3.34YY201 pKa = 9.53TSEE204 pKa = 4.05EE205 pKa = 4.46DD206 pKa = 3.34EE207 pKa = 5.01CEE209 pKa = 4.02LVPLSGDD216 pKa = 3.4TLPRR220 pKa = 11.84GEE222 pKa = 4.58IPNEE226 pKa = 3.87IVSVSATVNRR236 pKa = 11.84CLEE239 pKa = 4.06SSYY242 pKa = 10.76MSTDD246 pKa = 2.88IPDD249 pKa = 4.03LGRR252 pKa = 11.84FIEE255 pKa = 4.58EE256 pKa = 3.83PVEE259 pKa = 3.79FDD261 pKa = 3.11GFDD264 pKa = 2.89RR265 pKa = 11.84VVAVNDD271 pKa = 2.92WWSGMKK277 pKa = 10.15LVPNLGIPDD286 pKa = 4.29DD287 pKa = 4.4SDD289 pKa = 4.39VIFSGILSSAPEE301 pKa = 4.15VMQSAIDD308 pKa = 3.64EE309 pKa = 4.53FLPLHH314 pKa = 5.96HH315 pKa = 6.91QIDD318 pKa = 3.64DD319 pKa = 3.71RR320 pKa = 11.84FFQEE324 pKa = 3.88IVEE327 pKa = 4.31TSDD330 pKa = 3.8ISLEE334 pKa = 4.12LEE336 pKa = 3.98NCSFDD341 pKa = 4.01LSSFKK346 pKa = 10.5SWSSHH351 pKa = 5.29EE352 pKa = 4.49SGCCSSLNSGMTSTRR367 pKa = 11.84TNTFRR372 pKa = 11.84EE373 pKa = 3.74AALAIKK379 pKa = 9.01KK380 pKa = 10.45RR381 pKa = 11.84NMNVPAISSSCDD393 pKa = 2.8IGKK396 pKa = 10.1VSDD399 pKa = 3.94DD400 pKa = 3.53VVEE403 pKa = 4.44KK404 pKa = 10.49FFSRR408 pKa = 11.84IIDD411 pKa = 3.45VDD413 pKa = 3.83KK414 pKa = 11.42LIGLPIIGHH423 pKa = 6.41GEE425 pKa = 3.95LAWFADD431 pKa = 3.89YY432 pKa = 11.43LKK434 pKa = 10.63GKK436 pKa = 8.77PVNEE440 pKa = 4.5DD441 pKa = 3.23MFVDD445 pKa = 5.4PICLVSMDD453 pKa = 4.64KK454 pKa = 10.37YY455 pKa = 9.77RR456 pKa = 11.84HH457 pKa = 4.78MVKK460 pKa = 10.12SQLKK464 pKa = 8.47PVEE467 pKa = 4.55DD468 pKa = 3.5NSLAFEE474 pKa = 4.6RR475 pKa = 11.84PLPATITYY483 pKa = 9.28HH484 pKa = 7.58DD485 pKa = 4.21KK486 pKa = 11.19GKK488 pKa = 10.99VMSTSPLFLALMNRR502 pKa = 11.84LLMSLKK508 pKa = 10.5SKK510 pKa = 10.65ISIPTGKK517 pKa = 9.79FHH519 pKa = 7.59QLFSLDD525 pKa = 3.42AQVFDD530 pKa = 5.98SVLEE534 pKa = 3.99WKK536 pKa = 10.42EE537 pKa = 3.43IDD539 pKa = 3.52FSKK542 pKa = 10.58FDD544 pKa = 3.41KK545 pKa = 10.84SQQEE549 pKa = 4.02LHH551 pKa = 6.93HH552 pKa = 6.2EE553 pKa = 4.26VQKK556 pKa = 11.13KK557 pKa = 9.36IFLRR561 pKa = 11.84LGLPRR566 pKa = 11.84DD567 pKa = 4.16FCDD570 pKa = 2.6TWFTSHH576 pKa = 5.73VRR578 pKa = 11.84SHH580 pKa = 6.86ISDD583 pKa = 3.63PSGLRR588 pKa = 11.84FSVNFQRR595 pKa = 11.84RR596 pKa = 11.84TGDD599 pKa = 2.95AVTYY603 pKa = 10.41LGNTVVTLAVLSYY616 pKa = 11.04VYY618 pKa = 10.58DD619 pKa = 4.05LSDD622 pKa = 4.17PNVLMVVASGDD633 pKa = 3.49DD634 pKa = 3.66SLIGSYY640 pKa = 10.41RR641 pKa = 11.84PLDD644 pKa = 3.48RR645 pKa = 11.84SRR647 pKa = 11.84EE648 pKa = 4.22HH649 pKa = 6.87LCSTLFNFEE658 pKa = 4.24AKK660 pKa = 10.1FPHH663 pKa = 5.93NQPFICSKK671 pKa = 10.66FLLTMPTKK679 pKa = 9.87SGGRR683 pKa = 11.84RR684 pKa = 11.84VVAVPNPLKK693 pKa = 10.83LLIKK697 pKa = 10.55LGIRR701 pKa = 11.84NLQEE705 pKa = 3.97DD706 pKa = 4.47QFDD709 pKa = 3.0AWYY712 pKa = 8.69TSWIDD717 pKa = 3.8LIHH720 pKa = 6.2YY721 pKa = 9.51FNDD724 pKa = 2.8EE725 pKa = 3.98HH726 pKa = 7.66LISVVAEE733 pKa = 4.09MCSYY737 pKa = 10.92RR738 pKa = 11.84YY739 pKa = 9.58LRR741 pKa = 11.84KK742 pKa = 9.37PSMFLKK748 pKa = 10.59PAMCSFNNVFANKK761 pKa = 8.24TKK763 pKa = 10.52LMKK766 pKa = 10.45FLFPSMVLRR775 pKa = 11.84KK776 pKa = 9.77DD777 pKa = 3.03KK778 pKa = 10.81RR779 pKa = 11.84KK780 pKa = 9.88RR781 pKa = 11.84PPRR784 pKa = 11.84KK785 pKa = 9.33RR786 pKa = 3.3

Molecular weight: 89.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5QDM5|Q5QDM5_9BROM ATP-dependent helicase OS=Fragaria chiloensis latent virus OX=255238 PE=3 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84YY3 pKa = 9.02CRR5 pKa = 11.84CSLIVCRR12 pKa = 11.84ISSMSVVLIYY22 pKa = 10.99LFLSKK27 pKa = 10.44RR28 pKa = 11.84IPSVCLPIFIEE39 pKa = 4.15VFLRR43 pKa = 11.84CLTRR47 pKa = 11.84HH48 pKa = 6.33AVRR51 pKa = 11.84QAWKK55 pKa = 8.85VGVPVRR61 pKa = 11.84EE62 pKa = 4.29VPRR65 pKa = 11.84VRR67 pKa = 11.84PVMFVTKK74 pKa = 10.3FIMPDD79 pKa = 3.12LLIRR83 pKa = 11.84CLSVPVAGQIIPARR97 pKa = 11.84KK98 pKa = 7.94MSVNLFHH105 pKa = 7.36LAVTPYY111 pKa = 10.62RR112 pKa = 11.84EE113 pKa = 4.06VRR115 pKa = 11.84SQMRR119 pKa = 11.84SLALVLRR126 pKa = 11.84SIDD129 pKa = 2.87AWRR132 pKa = 11.84VLICLPIFPILDD144 pKa = 3.91DD145 pKa = 3.98LSKK148 pKa = 11.13NRR150 pKa = 11.84SNLMDD155 pKa = 3.46STVWLLSMTGGLGG168 pKa = 3.17

Molecular weight: 19.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2648

78

1083

441.3

49.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.382 ± 1.113

2.644 ± 0.332

6.156 ± 0.632

5.211 ± 0.527

5.249 ± 0.474

5.211 ± 0.587

2.228 ± 0.324

5.74 ± 0.489

5.098 ± 0.818

8.573 ± 0.762

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.185

3.776 ± 0.389

5.098 ± 0.641

2.53 ± 0.26

6.533 ± 1.074

8.988 ± 1.302

5.816 ± 0.702

8.119 ± 0.665

1.133 ± 0.169

2.795 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski