Microbacterium aerolatum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A511AC89|A0A511AC89_9MICO DNA-binding response regulator OS=Microbacterium aerolatum OX=153731 GN=MAE01_09510 PE=4 SV=1
MM1 pKa = 7.36HH2 pKa = 7.14SKK4 pKa = 10.63ARR6 pKa = 11.84LVAVGSAVAALALAGCSAGQANDD29 pKa = 3.79GGGDD33 pKa = 3.39SADD36 pKa = 4.13PIRR39 pKa = 11.84YY40 pKa = 8.86AVEE43 pKa = 4.25QPDD46 pKa = 3.76SMIPGNQFSSYY57 pKa = 10.83VILEE61 pKa = 4.5TIFAPLTQVDD71 pKa = 3.78EE72 pKa = 4.91DD73 pKa = 4.91GEE75 pKa = 4.7VSFVAAEE82 pKa = 4.22SVEE85 pKa = 4.36SDD87 pKa = 4.69DD88 pKa = 3.98AQTWTITLRR97 pKa = 11.84DD98 pKa = 3.26GWTFHH103 pKa = 7.39DD104 pKa = 4.96GEE106 pKa = 4.71PVTAQDD112 pKa = 6.11YY113 pKa = 10.51VDD115 pKa = 3.3TWNYY119 pKa = 9.32AAYY122 pKa = 9.96GPNAWVNGPQLANVAGYY139 pKa = 11.32ADD141 pKa = 4.85LNPAEE146 pKa = 4.78GEE148 pKa = 4.08PTATEE153 pKa = 3.88LAGLSVVDD161 pKa = 4.77DD162 pKa = 3.56RR163 pKa = 11.84TFTVEE168 pKa = 4.47LSAPDD173 pKa = 3.39RR174 pKa = 11.84QYY176 pKa = 11.59PLQLGIGQTGLYY188 pKa = 8.18PLPSEE193 pKa = 5.16ALDD196 pKa = 5.46DD197 pKa = 3.93LAAWEE202 pKa = 4.29EE203 pKa = 4.31APVGNGPFEE212 pKa = 4.4LTDD215 pKa = 3.33GWSEE219 pKa = 4.06SDD221 pKa = 3.93PIEE224 pKa = 4.06AVAYY228 pKa = 10.28DD229 pKa = 5.11DD230 pKa = 4.26YY231 pKa = 12.04AGDD234 pKa = 4.48APTLDD239 pKa = 3.21AVTFVPYY246 pKa = 10.77LDD248 pKa = 3.6TATAYY253 pKa = 9.58TDD255 pKa = 4.08ALAGDD260 pKa = 3.92IDD262 pKa = 3.8IAAIAAGQAVQARR275 pKa = 11.84NDD277 pKa = 3.65FGDD280 pKa = 3.51SVYY283 pKa = 10.98EE284 pKa = 3.84LDD286 pKa = 4.26APGVDD291 pKa = 4.12FLGFTLSDD299 pKa = 3.31PRR301 pKa = 11.84FSDD304 pKa = 3.12VRR306 pKa = 11.84VRR308 pKa = 11.84QAISMAIDD316 pKa = 3.26RR317 pKa = 11.84EE318 pKa = 4.48AINDD322 pKa = 3.71AVFGGSQVPATSLTAPSMPGDD343 pKa = 3.67PEE345 pKa = 4.75GVCGEE350 pKa = 4.2YY351 pKa = 10.99CEE353 pKa = 5.03FDD355 pKa = 3.66PDD357 pKa = 3.47AAKK360 pKa = 10.85ALLEE364 pKa = 4.11EE365 pKa = 4.8AGGFDD370 pKa = 4.8GEE372 pKa = 4.57MKK374 pKa = 10.5VHH376 pKa = 6.9FVANWGQEE384 pKa = 4.0DD385 pKa = 4.59YY386 pKa = 11.43FEE388 pKa = 6.6AIANQLRR395 pKa = 11.84QNLGIEE401 pKa = 4.28AVIASPSADD410 pKa = 2.97FAAFTDD416 pKa = 4.17AVQNGEE422 pKa = 3.75TDD424 pKa = 3.61GPFRR428 pKa = 11.84ARR430 pKa = 11.84WGALYY435 pKa = 9.57PSQQNTLTAVFTATGEE451 pKa = 4.54GNFGAGGYY459 pKa = 9.65SSPEE463 pKa = 3.5VDD465 pKa = 3.3EE466 pKa = 5.56LLRR469 pKa = 11.84QANAADD475 pKa = 3.77TLEE478 pKa = 4.89DD479 pKa = 3.57SLAGYY484 pKa = 9.31VAAQEE489 pKa = 5.37RR490 pKa = 11.84ILQDD494 pKa = 3.71FPTVPMFGNRR504 pKa = 11.84YY505 pKa = 9.82LYY507 pKa = 9.1VTSDD511 pKa = 4.41RR512 pKa = 11.84IAEE515 pKa = 4.06LHH517 pKa = 6.06TNAGAIDD524 pKa = 3.78VPKK527 pKa = 10.39IAVAGG532 pKa = 3.88

Molecular weight: 56.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A511AEF6|A0A511AEF6_9MICO DNA helicase OS=Microbacterium aerolatum OX=153731 GN=MAE01_17240 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3137

0

3137

990643

41

2029

315.8

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.305 ± 0.064

0.477 ± 0.009

6.409 ± 0.038

5.95 ± 0.035

3.152 ± 0.027

8.755 ± 0.036

2.009 ± 0.022

4.977 ± 0.035

2.07 ± 0.034

9.968 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.974 ± 0.018

2.004 ± 0.025

5.194 ± 0.03

2.871 ± 0.021

7.169 ± 0.054

5.606 ± 0.027

5.999 ± 0.031

8.666 ± 0.038

1.511 ± 0.018

1.934 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski