Kwoniella shandongensis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Kwoniella

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5M6BS84|A0A5M6BS84_9TREE Uncharacterized protein OS=Kwoniella shandongensis OX=1734106 GN=CI109_007192 PE=4 SV=1
MM1 pKa = 7.3FSKK4 pKa = 10.59FALVTLTAIIGAGVGVTAAPFTKK27 pKa = 9.8RR28 pKa = 11.84DD29 pKa = 3.94TNVLLYY35 pKa = 10.03SQKK38 pKa = 11.39DD39 pKa = 3.71NLCLSVEE46 pKa = 4.42TGAQANDD53 pKa = 3.14QTQAYY58 pKa = 8.87SNHH61 pKa = 5.97SSPYY65 pKa = 8.43LYY67 pKa = 10.36EE68 pKa = 4.03GLRR71 pKa = 11.84VVSLPCEE78 pKa = 4.24NSSTWDD84 pKa = 3.37VSQGNGSVYY93 pKa = 9.13VTNSDD98 pKa = 3.24QAFALEE104 pKa = 4.67AGPHH108 pKa = 5.49VDD110 pKa = 3.59NFQTLWVNKK119 pKa = 8.22TYY121 pKa = 10.06PGKK124 pKa = 10.06YY125 pKa = 9.04SQTFFYY131 pKa = 10.57TGDD134 pKa = 3.23RR135 pKa = 11.84RR136 pKa = 11.84LAVTNSTEE144 pKa = 4.55CVDD147 pKa = 3.9EE148 pKa = 4.63GDD150 pKa = 4.42NGAQEE155 pKa = 4.38YY156 pKa = 10.71SCTTGDD162 pKa = 3.89TNQIWDD168 pKa = 3.76ATSPNGPLDD177 pKa = 4.18PQPLTTFASSVSTAAATASPASASSDD203 pKa = 3.45VTSASSGDD211 pKa = 3.91DD212 pKa = 3.49PSTATADD219 pKa = 3.66ATSVTAAGATASTGSSPTDD238 pKa = 3.38NASGSSDD245 pKa = 2.63IAAAATTSPSDD256 pKa = 3.38TASAATASADD266 pKa = 3.27ITRR269 pKa = 11.84LASSPSSDD277 pKa = 3.05VAAAATASPAFTAATFALGGEE298 pKa = 4.71PEE300 pKa = 4.65SSTAPASAASDD311 pKa = 3.61SASASSCKK319 pKa = 10.02RR320 pKa = 11.84SGANN324 pKa = 3.14

Molecular weight:
32.52 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5M6BWC3|A0A5M6BWC3_9TREE L51_S25_CI-B8 domain-containing protein OS=Kwoniella shandongensis OX=1734106 GN=CI109_004470 PE=4 SV=1
MM1 pKa = 7.5PRR3 pKa = 11.84LPRR6 pKa = 11.84TAFLRR11 pKa = 11.84SLPLPPLQPRR21 pKa = 11.84LALPSVSRR29 pKa = 11.84TTLTPSTFTSSFASLRR45 pKa = 11.84LSPLNSSLITSSLRR59 pKa = 11.84PSISNLPTHH68 pKa = 6.83ILSSSSTSQIQLGGVRR84 pKa = 11.84GAAMGTFYY92 pKa = 10.92QPSQRR97 pKa = 11.84KK98 pKa = 8.72RR99 pKa = 11.84KK100 pKa = 8.95NKK102 pKa = 9.88HH103 pKa = 4.66GFLARR108 pKa = 11.84LRR110 pKa = 11.84GGKK113 pKa = 7.91NARR116 pKa = 11.84KK117 pKa = 8.1MLSRR121 pKa = 11.84RR122 pKa = 11.84LLKK125 pKa = 10.3GRR127 pKa = 11.84KK128 pKa = 8.56NLSHH132 pKa = 7.01

Molecular weight:
14.52 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7422

0

7422

3956821

40

4986

533.1

58.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.478 ± 0.026

0.911 ± 0.008

5.541 ± 0.021

6.405 ± 0.026

3.265 ± 0.017

7.519 ± 0.027

2.31 ± 0.013

4.609 ± 0.018

4.918 ± 0.024

8.724 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.043 ± 0.011

3.293 ± 0.013

6.793 ± 0.032

3.73 ± 0.023

6.026 ± 0.023

9.247 ± 0.041

6.299 ± 0.02

6.083 ± 0.019

1.351 ± 0.009

2.456 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski