Bacteriophage Reminis

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Gajwadongvirus; unclassified Gajwadongvirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2U9M6|A0A5Q2U9M6_9CAUD Uncharacterized protein OS=Bacteriophage Reminis OX=2662139 PE=4 SV=1
MM1 pKa = 7.52TYY3 pKa = 11.11ANKK6 pKa = 10.52EE7 pKa = 4.03EE8 pKa = 4.11ALEE11 pKa = 4.3AAAEE15 pKa = 4.01MALDD19 pKa = 4.39GKK21 pKa = 10.96LPTPDD26 pKa = 4.46EE27 pKa = 4.39FDD29 pKa = 3.38AMQSVGVDD37 pKa = 3.19YY38 pKa = 10.96KK39 pKa = 10.64IVYY42 pKa = 10.07ALLSEE47 pKa = 4.18VFYY50 pKa = 11.1DD51 pKa = 4.15GEE53 pKa = 4.16EE54 pKa = 4.31LEE56 pKa = 4.65EE57 pKa = 4.85SSDD60 pKa = 3.45AA61 pKa = 4.59

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2UAL7|A0A5Q2UAL7_9CAUD DNA-directed RNA polymerase OS=Bacteriophage Reminis OX=2662139 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.29LYY4 pKa = 10.29RR5 pKa = 11.84HH6 pKa = 6.3PLGGLFAITSLNTVLTYY23 pKa = 11.25NKK25 pKa = 9.67IGKK28 pKa = 8.52CWKK31 pKa = 9.53KK32 pKa = 10.63SAWNGDD38 pKa = 3.52LDD40 pKa = 4.58KK41 pKa = 11.28LLPEE45 pKa = 4.93LKK47 pKa = 10.59LVGNNFRR54 pKa = 11.84RR55 pKa = 11.84IVRR58 pKa = 3.7

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

13837

40

1288

226.8

25.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.542 ± 0.424

0.997 ± 0.142

6.092 ± 0.213

6.461 ± 0.359

3.585 ± 0.171

7.061 ± 0.273

1.995 ± 0.213

5.572 ± 0.197

6.837 ± 0.344

8.333 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.144 ± 0.168

4.459 ± 0.204

3.433 ± 0.169

4.423 ± 0.483

4.676 ± 0.201

6.504 ± 0.283

5.839 ± 0.409

6.757 ± 0.304

1.431 ± 0.139

3.859 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski