Phaseolus vulgaris (Kidney bean) (French bean)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V7C7Q6|V7C7Q6_PHAVU Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_003G094600g PE=4 SV=1
MM1 pKa = 6.98FTIEE5 pKa = 4.41CQCHH9 pKa = 5.6EE10 pKa = 4.76SATMLIPEE18 pKa = 4.91PFVSHH23 pKa = 7.38LSTCSAEE30 pKa = 4.41HH31 pKa = 6.66PRR33 pKa = 11.84HH34 pKa = 5.01GHH36 pKa = 5.46VPDD39 pKa = 4.2TSSQPMTCTDD49 pKa = 3.24STTSSVGGDD58 pKa = 3.33NTDD61 pKa = 2.89GTTINVGGNSTNGTTSTVGGDD82 pKa = 3.62STDD85 pKa = 4.02GPSSTVSRR93 pKa = 11.84DD94 pKa = 3.27STDD97 pKa = 2.93GSTSTVGGNGTDD109 pKa = 3.7GATSTVGGNSTDD121 pKa = 4.07IFCCDD126 pKa = 3.47NSTDD130 pKa = 3.77GATSGTTSVGRR141 pKa = 11.84GKK143 pKa = 10.67RR144 pKa = 11.84SDD146 pKa = 3.13GTTRR150 pKa = 11.84TVGGNSTDD158 pKa = 3.67GPTSTLGGNSTDD170 pKa = 4.21DD171 pKa = 3.6PTSVFGGNSTDD182 pKa = 3.65GATSTVGGNTTDD194 pKa = 3.68GATSTVGGNTTDD206 pKa = 3.68GATSTVGGKK215 pKa = 6.38TTDD218 pKa = 3.54GATSTVGGNRR228 pKa = 11.84TDD230 pKa = 3.69GATSTVGCKK239 pKa = 8.17STNGTTSTAGCNSIDD254 pKa = 3.84GATIDD259 pKa = 4.28VGGNSTNGTISIVGGDD275 pKa = 3.75STDD278 pKa = 3.78GPTSTVGGNSTDD290 pKa = 3.59GATSTVVGNSSDD302 pKa = 3.61GATSTVGCNSTDD314 pKa = 3.4GATSTVGGNSTDD326 pKa = 3.59GATSTVGGNSTDD338 pKa = 3.59GATSTVGGNSTDD350 pKa = 3.59GATSTVGGNSTVGSTSTVGGYY371 pKa = 7.85STVGATSTVGCNSTDD386 pKa = 3.53GTPSTVGGDD395 pKa = 3.58STDD398 pKa = 2.95GSTVDD403 pKa = 3.16VGGNNTISTVGGEE416 pKa = 4.37STDD419 pKa = 3.89GPTSTVGGHH428 pKa = 6.34SNDD431 pKa = 3.95CATSTVGGNSTDD443 pKa = 3.1GTTSVVGGNRR453 pKa = 11.84SDD455 pKa = 3.52GTTSTVVVNSTDD467 pKa = 3.55YY468 pKa = 10.16ATSTGNDD475 pKa = 3.58GPNNTVGGNNTDD487 pKa = 3.84GGNSTDD493 pKa = 3.44GSTSTVVGNSTCSGG507 pKa = 3.32

Molecular weight: 47.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V7CN81|V7CN81_PHAVU PORR domain-containing protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_002G252200g PE=4 SV=1
MM1 pKa = 7.47AGSRR5 pKa = 11.84ASSMINGRR13 pKa = 11.84SIPRR17 pKa = 11.84RR18 pKa = 11.84FSGRR22 pKa = 11.84PSPKK26 pKa = 9.16RR27 pKa = 11.84GQVKK31 pKa = 10.2VGIVLGLANSVASIFSRR48 pKa = 11.84NRR50 pKa = 11.84TRR52 pKa = 11.84ASATLSHH59 pKa = 7.2LAHH62 pKa = 7.1

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28020

2481

30501

12704981

8

5429

416.5

46.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.419 ± 0.013

1.917 ± 0.008

5.212 ± 0.01

6.465 ± 0.015

4.337 ± 0.009

6.384 ± 0.013

2.535 ± 0.007

5.34 ± 0.009

6.18 ± 0.013

9.832 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.005

4.651 ± 0.009

4.83 ± 0.013

3.679 ± 0.01

5.109 ± 0.011

9.054 ± 0.017

4.981 ± 0.009

6.604 ± 0.01

1.282 ± 0.005

2.798 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski