Pseudomonas phage vB_PaeS_PAO1_Ab18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Abidjanvirus; Pseudomonas virus Ab18

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1IVS3|A0A0A1IVS3_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeS_PAO1_Ab18 OX=1548905 GN=ORF56 PE=4 SV=1
MM1 pKa = 7.42NYY3 pKa = 10.15EE4 pKa = 3.85MTKK7 pKa = 10.04ILVTALVADD16 pKa = 4.69TSATVEE22 pKa = 3.94WAQDD26 pKa = 3.62VLGGDD31 pKa = 3.58AFGVAVSLGDD41 pKa = 3.64KK42 pKa = 10.75LFLVYY47 pKa = 10.78QMDD50 pKa = 4.39NDD52 pKa = 4.03PQEE55 pKa = 4.25DD56 pKa = 3.74WLVDD60 pKa = 3.25IMEE63 pKa = 4.88TEE65 pKa = 4.24GEE67 pKa = 4.06MAYY70 pKa = 10.78CPDD73 pKa = 3.38VQGFASQAAALRR85 pKa = 11.84YY86 pKa = 8.7LASKK90 pKa = 9.97II91 pKa = 3.75

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1IV60|A0A0A1IV60_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeS_PAO1_Ab18 OX=1548905 GN=ORF42 PE=4 SV=1
MM1 pKa = 7.22TLDD4 pKa = 6.25AILLWSGWATLVLGVLFSIASGLALVLVLLDD35 pKa = 3.85YY36 pKa = 10.88LGKK39 pKa = 10.21RR40 pKa = 11.84LWHH43 pKa = 6.15RR44 pKa = 11.84TLKK47 pKa = 10.67FYY49 pKa = 10.81DD50 pKa = 3.43LHH52 pKa = 5.59EE53 pKa = 4.29VARR56 pKa = 11.84VVRR59 pKa = 11.84EE60 pKa = 3.96AEE62 pKa = 4.23LEE64 pKa = 4.18GKK66 pKa = 8.04LTRR69 pKa = 11.84RR70 pKa = 11.84APPEE74 pKa = 3.8PPRR77 pKa = 11.84KK78 pKa = 9.46RR79 pKa = 11.84RR80 pKa = 11.84GLWKK84 pKa = 10.37KK85 pKa = 10.44

Molecular weight: 9.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

17719

55

825

233.1

26.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.679 ± 0.403

1.106 ± 0.107

5.666 ± 0.206

7.777 ± 0.319

3.629 ± 0.181

7.856 ± 0.224

2.009 ± 0.154

3.9 ± 0.185

4.876 ± 0.265

9.306 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.109

3.403 ± 0.192

4.854 ± 0.241

3.973 ± 0.167

6.863 ± 0.243

5.621 ± 0.276

5.39 ± 0.251

6.964 ± 0.212

2.116 ± 0.156

2.912 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski