Carrot mottle mimic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q66127|Q66127_9TOMB RNA-directed RNA polymerase (Fragment) OS=Carrot mottle mimic virus OX=47736 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 6.64HH3 pKa = 5.96ITKK6 pKa = 10.15FLRR9 pKa = 11.84MDD11 pKa = 4.23FSPKK15 pKa = 10.39DD16 pKa = 3.41GVLTRR21 pKa = 11.84RR22 pKa = 11.84QCAEE26 pKa = 4.11RR27 pKa = 11.84FDD29 pKa = 4.32DD30 pKa = 3.7TWGLLITQARR40 pKa = 11.84MTTAHH45 pKa = 6.33TADD48 pKa = 2.91MCAWYY53 pKa = 9.96EE54 pKa = 4.04GSRR57 pKa = 11.84EE58 pKa = 4.08TNQYY62 pKa = 10.81LPLEE66 pKa = 4.22QFVDD70 pKa = 3.45SRR72 pKa = 11.84DD73 pKa = 3.5ASKK76 pKa = 10.6EE77 pKa = 4.02AEE79 pKa = 4.28DD80 pKa = 4.93KK81 pKa = 11.0VDD83 pKa = 4.16KK84 pKa = 10.92LSSPPTRR91 pKa = 11.84GCGGTTVNADD101 pKa = 3.05GGLVEE106 pKa = 5.76NPLPTNRR113 pKa = 11.84DD114 pKa = 3.26EE115 pKa = 4.57MPASSMQMNAPSGGIAVASVNSEE138 pKa = 3.69VARR141 pKa = 11.84WVFALPRR148 pKa = 11.84EE149 pKa = 3.91LDD151 pKa = 3.71EE152 pKa = 6.05PIMEE156 pKa = 4.42TPVDD160 pKa = 3.88PSAPTEE166 pKa = 4.14AEE168 pKa = 3.86RR169 pKa = 11.84EE170 pKa = 4.08LARR173 pKa = 11.84VLNNTVAVGDD183 pKa = 3.97TASALSTIQEE193 pKa = 4.32EE194 pKa = 4.42PQHH197 pKa = 5.93EE198 pKa = 4.87VIAVPGTAVGVVHH211 pKa = 6.49QEE213 pKa = 3.69DD214 pKa = 3.99PAPVEE219 pKa = 4.47GSPSTRR225 pKa = 11.84DD226 pKa = 2.97AAMEE230 pKa = 4.11GDD232 pKa = 4.15AGHH235 pKa = 6.46SAHH238 pKa = 7.29AGDD241 pKa = 4.54DD242 pKa = 3.51TDD244 pKa = 5.57ASVEE248 pKa = 4.07MPTTVEE254 pKa = 3.57AAGPLMEE261 pKa = 5.71EE262 pKa = 4.97GPSMAHH268 pKa = 5.03LTSYY272 pKa = 8.94TVAMEE277 pKa = 3.71LRR279 pKa = 11.84SRR281 pKa = 11.84FGLRR285 pKa = 11.84PASPANIEE293 pKa = 3.74LGARR297 pKa = 11.84VARR300 pKa = 11.84EE301 pKa = 3.63ILKK304 pKa = 8.97TAGARR309 pKa = 11.84RR310 pKa = 11.84QDD312 pKa = 3.32NYY314 pKa = 11.58YY315 pKa = 10.59LAQQAVKK322 pKa = 10.34FFLTPTLLDD331 pKa = 3.47VVYY334 pKa = 8.98STPIRR339 pKa = 11.84DD340 pKa = 4.68FYY342 pKa = 11.93

Molecular weight: 37.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q66129|Q66129_9TOMB Cell-to-cell movement protein OS=Carrot mottle mimic virus OX=47736 PE=4 SV=1
MM1 pKa = 7.52NLQIQPIGDD10 pKa = 3.89GNNSSRR16 pKa = 11.84NSRR19 pKa = 11.84RR20 pKa = 11.84PNQPSPRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84DD30 pKa = 3.3PRR32 pKa = 11.84QASGSWLWCPNTSPRR47 pKa = 11.84QQYY50 pKa = 9.09PYY52 pKa = 9.45MYY54 pKa = 9.81ATIPPRR60 pKa = 11.84APTNGPGFLYY70 pKa = 10.41PEE72 pKa = 4.48MAHH75 pKa = 5.67EE76 pKa = 4.73AQWGSPVYY84 pKa = 10.4RR85 pKa = 11.84EE86 pKa = 4.1ASSGIPATRR95 pKa = 11.84ARR97 pKa = 11.84RR98 pKa = 11.84NRR100 pKa = 11.84GRR102 pKa = 11.84GSVVAPRR109 pKa = 11.84YY110 pKa = 9.95GPVGPGTGRR119 pKa = 11.84PQSVTEE125 pKa = 4.11PQRR128 pKa = 11.84GTAAGRR134 pKa = 11.84FLSEE138 pKa = 3.92LLYY141 pKa = 10.72SFEE144 pKa = 4.37RR145 pKa = 11.84FGRR148 pKa = 11.84EE149 pKa = 3.76CPAMFLPSDD158 pKa = 3.79PASRR162 pKa = 11.84HH163 pKa = 5.55PPEE166 pKa = 5.54DD167 pKa = 2.8WLQRR171 pKa = 11.84LLPALNVEE179 pKa = 4.13PSHH182 pKa = 7.02RR183 pKa = 11.84RR184 pKa = 11.84EE185 pKa = 4.31GPTLPSNRR193 pKa = 11.84AGVCAHH199 pKa = 6.67SKK201 pKa = 10.89ALDD204 pKa = 3.57KK205 pKa = 11.21EE206 pKa = 4.5LVADD210 pKa = 4.29TGTTAAVSEE219 pKa = 4.32RR220 pKa = 11.84RR221 pKa = 11.84ADD223 pKa = 3.47EE224 pKa = 4.03PTRR227 pKa = 11.84KK228 pKa = 9.58EE229 pKa = 4.17PEE231 pKa = 4.08GQPITEE237 pKa = 4.4APSTGLCSKK246 pKa = 9.62PHH248 pKa = 5.59TRR250 pKa = 11.84GEE252 pKa = 4.23QCSDD256 pKa = 3.34PTLHH260 pKa = 6.79FF261 pKa = 4.51

Molecular weight: 28.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1434

261

569

358.5

39.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.229 ± 1.275

1.953 ± 0.532

5.23 ± 0.81

5.718 ± 0.871

3.766 ± 0.81

7.043 ± 0.312

2.301 ± 0.204

3.696 ± 0.668

3.417 ± 0.908

8.368 ± 1.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.563

3.487 ± 0.432

8.298 ± 1.159

3.975 ± 0.44

7.95 ± 0.791

7.462 ± 0.963

5.858 ± 1.024

7.043 ± 1.097

1.255 ± 0.125

2.58 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski