bacterium HR23

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5YF25|A0A2H5YF25_9BACT Glucose 1-dehydrogenase OS=bacterium HR23 OX=2035418 GN=HRbin23_01662 PE=4 SV=1
MM1 pKa = 8.01PEE3 pKa = 3.3QTAQQKK9 pKa = 9.84PRR11 pKa = 11.84VTTEE15 pKa = 3.57QVIEE19 pKa = 4.14ALRR22 pKa = 11.84EE23 pKa = 4.08VYY25 pKa = 10.54DD26 pKa = 3.67PEE28 pKa = 5.14IPVNIYY34 pKa = 10.89DD35 pKa = 4.15LGLVYY40 pKa = 10.49DD41 pKa = 4.25VQVDD45 pKa = 3.77DD46 pKa = 4.05NNRR49 pKa = 11.84VYY51 pKa = 11.25VAMTLTFPGCGMGPYY66 pKa = 9.58IAQQAEE72 pKa = 3.73WRR74 pKa = 11.84IAEE77 pKa = 4.05IEE79 pKa = 4.19GVEE82 pKa = 4.15DD83 pKa = 3.68VQVEE87 pKa = 4.32LVWDD91 pKa = 4.5PPWTPDD97 pKa = 3.29MIRR100 pKa = 11.84PEE102 pKa = 4.27GKK104 pKa = 9.92KK105 pKa = 10.59LLGLEE110 pKa = 4.31EE111 pKa = 4.14

Molecular weight: 12.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5YDH7|A0A2H5YDH7_9BACT Hypoxanthine phosphoribosyltransferase OS=bacterium HR23 OX=2035418 GN=hpt_2 PE=3 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.97RR4 pKa = 11.84TYY6 pKa = 8.89QPKK9 pKa = 8.51RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84QRR15 pKa = 11.84VHH17 pKa = 6.43GFLARR22 pKa = 11.84MATRR26 pKa = 11.84GGRR29 pKa = 11.84KK30 pKa = 7.38VLKK33 pKa = 9.95RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84LAGRR40 pKa = 11.84WRR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1642

0

1642

473262

29

1371

288.2

31.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.613 ± 0.063

1.006 ± 0.023

4.099 ± 0.036

6.226 ± 0.053

3.322 ± 0.036

9.002 ± 0.057

2.28 ± 0.031

4.175 ± 0.041

2.595 ± 0.041

11.594 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.987 ± 0.023

1.887 ± 0.026

6.725 ± 0.061

3.905 ± 0.043

8.387 ± 0.055

4.296 ± 0.038

5.039 ± 0.052

8.731 ± 0.06

1.614 ± 0.029

2.517 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski