Amoebophilus asiaticus (strain 5a2)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Amoebophilaceae; Candidatus Amoebophilus; Candidatus Amoebophilus asiaticus

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3ERG0|B3ERG0_AMOA5 Uncharacterized protein OS=Amoebophilus asiaticus (strain 5a2) OX=452471 GN=Aasi_0392 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.04LASYY10 pKa = 10.82LDD12 pKa = 4.11DD13 pKa = 5.06APWPATKK20 pKa = 10.19EE21 pKa = 3.92QLIDD25 pKa = 3.46FADD28 pKa = 4.99RR29 pKa = 11.84IGAPIEE35 pKa = 4.06VIEE38 pKa = 4.15NLEE41 pKa = 3.89EE42 pKa = 5.89LEE44 pKa = 5.54DD45 pKa = 4.78DD46 pKa = 3.7DD47 pKa = 5.92HH48 pKa = 7.95PYY50 pKa = 11.49DD51 pKa = 4.53SIEE54 pKa = 4.5EE55 pKa = 3.98IWPEE59 pKa = 4.09YY60 pKa = 7.32PTKK63 pKa = 10.82EE64 pKa = 3.61DD65 pKa = 3.56FLFNEE70 pKa = 4.91DD71 pKa = 3.49EE72 pKa = 4.45YY73 pKa = 12.0

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3ESA9|B3ESA9_AMOA5 Smr domain-containing protein OS=Amoebophilus asiaticus (strain 5a2) OX=452471 GN=Aasi_0727 PE=4 SV=1
MM1 pKa = 7.53PHH3 pKa = 6.91PKK5 pKa = 9.55RR6 pKa = 11.84RR7 pKa = 11.84QSRR10 pKa = 11.84TRR12 pKa = 11.84RR13 pKa = 11.84DD14 pKa = 2.9KK15 pKa = 11.23RR16 pKa = 11.84RR17 pKa = 11.84THH19 pKa = 7.16DD20 pKa = 3.68KK21 pKa = 11.45APMPNLAVCPTTGEE35 pKa = 3.69LHH37 pKa = 6.04LPHH40 pKa = 7.02RR41 pKa = 11.84AFWYY45 pKa = 9.83EE46 pKa = 3.58DD47 pKa = 2.81KK48 pKa = 10.89LYY50 pKa = 10.89YY51 pKa = 9.44KK52 pKa = 10.39GKK54 pKa = 10.64VVMEE58 pKa = 4.72RR59 pKa = 11.84NASS62 pKa = 3.32

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1253

0

1253

470492

38

4520

375.5

42.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.211 ± 0.071

0.946 ± 0.021

4.591 ± 0.047

6.652 ± 0.065

3.884 ± 0.049

5.838 ± 0.058

2.402 ± 0.037

8.25 ± 0.066

7.96 ± 0.072

10.593 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.028

5.085 ± 0.054

3.543 ± 0.036

5.007 ± 0.063

3.949 ± 0.044

5.992 ± 0.058

5.501 ± 0.054

5.579 ± 0.046

0.978 ± 0.023

4.108 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski