Sus scrofa (Pig)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria;

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A287ASX2|A0A287ASX2_PIG Isoform of A0A287A892 Ubiquitin specific peptidase 19 OS=Sus scrofa OX=9823 GN=USP19 PE=1 SV=2
MM1 pKa = 7.44KK2 pKa = 10.24VLKK5 pKa = 9.38FACKK9 pKa = 9.18VQKK12 pKa = 10.31KK13 pKa = 10.21DD14 pKa = 3.49PKK16 pKa = 10.72DD17 pKa = 2.76WAAQYY22 pKa = 10.63RR23 pKa = 11.84EE24 pKa = 4.28AVEE27 pKa = 4.36MEE29 pKa = 4.14VQAAAVAVAEE39 pKa = 4.18AEE41 pKa = 4.02ARR43 pKa = 11.84AEE45 pKa = 3.93ARR47 pKa = 11.84AQMGIGEE54 pKa = 4.33EE55 pKa = 4.2AVAGPWNWDD64 pKa = 3.77DD65 pKa = 4.4MDD67 pKa = 4.66IDD69 pKa = 3.97CLTRR73 pKa = 11.84EE74 pKa = 4.29EE75 pKa = 5.22LGDD78 pKa = 4.64DD79 pKa = 3.33AQAWSRR85 pKa = 11.84FSFEE89 pKa = 3.54IEE91 pKa = 4.62ARR93 pKa = 11.84DD94 pKa = 3.67QEE96 pKa = 4.36NANASTNIDD105 pKa = 3.84FSRR108 pKa = 11.84GASTRR113 pKa = 11.84ANFSDD118 pKa = 3.96GASICFSGAPSPSDD132 pKa = 3.23GFGGRR137 pKa = 11.84DD138 pKa = 3.53GISFGSTCSTSATFSSTASISFGGTHH164 pKa = 5.11YY165 pKa = 9.96TSGSFSSAANICFGGMPNTCSTFSGGASISFGGAASTSSSFSSEE209 pKa = 3.33ASISFGGTPCTSANFAGGVSPSFSGPLNTSTSFSGGASSGFGGTLSATAGFNDD262 pKa = 4.03ALITTTSFGSTLGTSAVFSGALSTSTAFGGTLSTSVYY299 pKa = 10.46FGGSPSSSASFGGTLSTSICFSGSPSTSTGFDD331 pKa = 3.31GVFSTSVSFGGSSSTSTDD349 pKa = 3.51FSGTLSTSVCFGDD362 pKa = 3.96SLSTSSSFGGALSTSVGFGGALSTSVGFGGALNTSAGFSSAVSINTGFNSVPSTNSGFSSVFSTSADD429 pKa = 3.29FSGALNTTTDD439 pKa = 3.86FGSASSTSIGFGGAPSTSFCFGSLSNTNLCFGGPPSTNTCFSGPTNASFGDD490 pKa = 4.03GLSTGAGFSFGDD502 pKa = 3.96GLNTSTGFSGGLSSSSSFGGGLSTSTGFGGGLISSDD538 pKa = 3.56VFGGGLGTSAGFGSTLGTRR557 pKa = 11.84ANFCSSLSISDD568 pKa = 3.96GFGSEE573 pKa = 4.63PNTSFDD579 pKa = 3.87GGLSTIIGFASSSNTSTGFTGEE601 pKa = 4.39PSTSTSFIGGPSSIVGFGSGLSTDD625 pKa = 3.77AGFNSGPSNAAGFGGGGTSLGACSFSYY652 pKa = 10.98GG653 pKa = 3.4

Molecular weight: 63.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286ZQ31|A0A286ZQ31_PIG Phosphoglycerate mutase OS=Sus scrofa OX=9823 GN=BPGM PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22165

27700

49865

29285362

3

7797

587.3

65.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.064 ± 0.013

2.171 ± 0.01

4.825 ± 0.008

7.126 ± 0.013

3.659 ± 0.007

6.54 ± 0.014

2.554 ± 0.005

4.446 ± 0.01

5.823 ± 0.013

9.974 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.004

3.589 ± 0.008

6.278 ± 0.017

4.741 ± 0.01

5.725 ± 0.011

8.265 ± 0.013

5.237 ± 0.007

6.006 ± 0.009

1.212 ± 0.004

2.629 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski