Pseudomonas phage vB_PaeP_C2-10_Ab22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Bruynoghevirus; Pseudomonas virus Ab22

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1IWR0|A0A0A1IWR0_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_C2-10_Ab22 OX=1548906 GN=ORF39 PE=4 SV=1
MM1 pKa = 7.09TSKK4 pKa = 10.76KK5 pKa = 10.32EE6 pKa = 4.04SLEE9 pKa = 3.95EE10 pKa = 3.74QALKK14 pKa = 10.64EE15 pKa = 3.78IALEE19 pKa = 4.34KK20 pKa = 10.35EE21 pKa = 4.86FSGSWGGPEE30 pKa = 4.13IDD32 pKa = 5.3ADD34 pKa = 4.14DD35 pKa = 4.73FPLGSACGLDD45 pKa = 3.54PEE47 pKa = 4.85VCEE50 pKa = 4.52SCQQ53 pKa = 3.52

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1IUQ0|A0A0A1IUQ0_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_C2-10_Ab22 OX=1548906 GN=ORF23 PE=4 SV=1
MM1 pKa = 7.7GKK3 pKa = 9.85RR4 pKa = 11.84KK5 pKa = 9.69VFYY8 pKa = 10.55EE9 pKa = 4.02RR10 pKa = 11.84LCSNGEE16 pKa = 3.75LTLNGNLKK24 pKa = 8.43ATKK27 pKa = 10.24VSAPTKK33 pKa = 10.02GKK35 pKa = 6.62QQRR38 pKa = 11.84RR39 pKa = 11.84GAFF42 pKa = 3.15

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13934

42

1055

196.3

21.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.418 ± 0.609

1.012 ± 0.159

5.914 ± 0.25

7.191 ± 0.429

3.854 ± 0.212

8.246 ± 0.359

1.974 ± 0.212

5.174 ± 0.201

5.813 ± 0.286

8.153 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.184

4.356 ± 0.218

4.263 ± 0.239

4.127 ± 0.277

5.957 ± 0.345

5.792 ± 0.273

5.167 ± 0.293

6.954 ± 0.227

1.701 ± 0.139

3.208 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski