Betaproteobacteria bacterium UKL13-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2745 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142LH60|A0A142LH60_9PROT Methylase OS=Betaproteobacteria bacterium UKL13-2 OX=1690485 GN=AEM42_02510 PE=4 SV=1
MM1 pKa = 6.11TTAAATKK8 pKa = 8.51TWMCLVCGWIYY19 pKa = 11.2NEE21 pKa = 3.91IEE23 pKa = 3.89GWPEE27 pKa = 3.56DD28 pKa = 4.31GIAPGTAWADD38 pKa = 3.57VPVGWACPEE47 pKa = 3.88CGARR51 pKa = 11.84KK52 pKa = 9.51EE53 pKa = 3.97DD54 pKa = 3.81FEE56 pKa = 4.37MVEE59 pKa = 4.0II60 pKa = 4.86

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142LGU4|A0A142LGU4_9PROT Uncharacterized protein OS=Betaproteobacteria bacterium UKL13-2 OX=1690485 GN=AEM42_01745 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.86QPSVARR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.08KK14 pKa = 7.52THH16 pKa = 5.12GFRR19 pKa = 11.84VRR21 pKa = 11.84MQTAGGRR28 pKa = 11.84KK29 pKa = 8.95VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2745

0

2745

861845

44

4192

314.0

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.446 ± 0.052

0.935 ± 0.017

5.31 ± 0.038

5.284 ± 0.048

3.917 ± 0.031

7.672 ± 0.05

2.096 ± 0.027

5.507 ± 0.037

4.486 ± 0.044

9.732 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.026

3.379 ± 0.035

4.758 ± 0.028

3.555 ± 0.027

6.135 ± 0.05

5.938 ± 0.043

5.905 ± 0.09

7.586 ± 0.041

1.303 ± 0.02

2.474 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski