Magnaporthe oryzae RNA virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7RYK7|A0A0A7RYK7_9VIRU RNA-dependent RNA polymerase OS=Magnaporthe oryzae RNA virus OX=1580607 GN=RdRp PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84TACPTSFVGMWVPAVHH18 pKa = 7.25ADD20 pKa = 3.68CPHH23 pKa = 5.93NQVASLLKK31 pKa = 9.42RR32 pKa = 11.84TLGPVPDD39 pKa = 4.66PVHH42 pKa = 6.62EE43 pKa = 4.23PVGPTVKK50 pKa = 10.46AVFSRR55 pKa = 11.84LRR57 pKa = 11.84ALAARR62 pKa = 11.84SGVSRR67 pKa = 11.84WSLRR71 pKa = 11.84RR72 pKa = 11.84TAEE75 pKa = 4.06SYY77 pKa = 10.54KK78 pKa = 10.22GALGRR83 pKa = 11.84RR84 pKa = 11.84YY85 pKa = 9.19MEE87 pKa = 4.26AEE89 pKa = 3.45RR90 pKa = 11.84SLRR93 pKa = 11.84VDD95 pKa = 3.46GPVNSGDD102 pKa = 3.3TYY104 pKa = 11.36LRR106 pKa = 11.84PFLKK110 pKa = 10.59AEE112 pKa = 4.14KK113 pKa = 9.99VKK115 pKa = 11.01VMDD118 pKa = 4.0KK119 pKa = 10.3QSKK122 pKa = 9.27PRR124 pKa = 11.84MIFPRR129 pKa = 11.84SPRR132 pKa = 11.84YY133 pKa = 9.31NLSIASRR140 pKa = 11.84LKK142 pKa = 10.7PFEE145 pKa = 3.71HH146 pKa = 6.45WVWGRR151 pKa = 11.84LTTQVVLGTGVGRR164 pKa = 11.84VVAKK168 pKa = 10.32GLNPRR173 pKa = 11.84QRR175 pKa = 11.84ANLIVRR181 pKa = 11.84KK182 pKa = 9.49FRR184 pKa = 11.84ALDD187 pKa = 3.53DD188 pKa = 4.25CVCVEE193 pKa = 4.11VDD195 pKa = 3.6GKK197 pKa = 10.99AFEE200 pKa = 4.12AHH202 pKa = 5.87VGEE205 pKa = 4.52DD206 pKa = 3.39QLLEE210 pKa = 3.85EE211 pKa = 4.66HH212 pKa = 6.39GVYY215 pKa = 9.77EE216 pKa = 4.76AAFPGDD222 pKa = 3.43WEE224 pKa = 4.25LQRR227 pKa = 11.84LLRR230 pKa = 11.84VQLSLRR236 pKa = 11.84GKK238 pKa = 9.06LPCGAEE244 pKa = 4.05FTRR247 pKa = 11.84PGGRR251 pKa = 11.84ASGDD255 pKa = 3.49FNTGMGNSLIMLAVVGAVMKK275 pKa = 10.12EE276 pKa = 3.9LAPGAFDD283 pKa = 5.68LLVDD287 pKa = 4.48GDD289 pKa = 3.96NALVFMPRR297 pKa = 11.84GLLGLVRR304 pKa = 11.84ANFEE308 pKa = 4.03RR309 pKa = 11.84RR310 pKa = 11.84VLEE313 pKa = 4.06VSGHH317 pKa = 6.02EE318 pKa = 4.08IQLEE322 pKa = 4.58SPCSVIEE329 pKa = 4.24EE330 pKa = 4.08IRR332 pKa = 11.84FGQSAPINLGGSRR345 pKa = 11.84GWTMVRR351 pKa = 11.84DD352 pKa = 3.74PRR354 pKa = 11.84KK355 pKa = 10.33VISQALSSHH364 pKa = 5.72RR365 pKa = 11.84WLRR368 pKa = 11.84EE369 pKa = 3.41PTFASEE375 pKa = 4.74WIRR378 pKa = 11.84GVAACEE384 pKa = 3.99LSLSRR389 pKa = 11.84GVPVLQAWSASLQRR403 pKa = 11.84VYY405 pKa = 10.92GGPGGVRR412 pKa = 11.84EE413 pKa = 4.76HH414 pKa = 6.78PHH416 pKa = 5.64TDD418 pKa = 3.06YY419 pKa = 10.85FYY421 pKa = 10.59QGAWFADD428 pKa = 3.65VSQSVEE434 pKa = 3.86VTQQARR440 pKa = 11.84ISFWAAFGVAPDD452 pKa = 3.83EE453 pKa = 4.06QLALEE458 pKa = 4.81GGAFEE463 pKa = 5.13PSSEE467 pKa = 4.06DD468 pKa = 3.33YY469 pKa = 10.99EE470 pKa = 4.49RR471 pKa = 11.84VVLEE475 pKa = 4.01SFQWRR480 pKa = 11.84DD481 pKa = 3.42MPPGVAEE488 pKa = 4.41PLADD492 pKa = 3.67SLL494 pKa = 4.89

Molecular weight: 54.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7RYK7|A0A0A7RYK7_9VIRU RNA-dependent RNA polymerase OS=Magnaporthe oryzae RNA virus OX=1580607 GN=RdRp PE=4 SV=1
MM1 pKa = 7.71AFYY4 pKa = 10.71FDD6 pKa = 4.05PTYY9 pKa = 10.8HH10 pKa = 7.37ALGLNADD17 pKa = 3.44RR18 pKa = 11.84MMLGEE23 pKa = 4.32ALFVVKK29 pKa = 10.55VAGCFAAPVLISGVATVLRR48 pKa = 11.84GIFAVPPPGSWSPRR62 pKa = 11.84GFLTDD67 pKa = 3.18IGRR70 pKa = 11.84RR71 pKa = 11.84FATPEE76 pKa = 3.89TDD78 pKa = 3.41RR79 pKa = 11.84SGCGAIAPSSAVGITRR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84ARR101 pKa = 11.84WVRR104 pKa = 11.84ALQDD108 pKa = 3.48MLGGPDD114 pKa = 3.41GCVGRR119 pKa = 11.84LFRR122 pKa = 11.84GRR124 pKa = 11.84WTPDD128 pKa = 2.85LPSSKK133 pKa = 9.66RR134 pKa = 11.84TDD136 pKa = 3.27VQNYY140 pKa = 9.3LLASIEE146 pKa = 4.16NGVKK150 pKa = 10.03ILGGGSEE157 pKa = 4.4KK158 pKa = 10.59VAPDD162 pKa = 3.6RR163 pKa = 11.84PSLDD167 pKa = 2.7GLYY170 pKa = 10.32FVVEE174 pKa = 3.9TSEE177 pKa = 4.5RR178 pKa = 11.84SVEE181 pKa = 4.18LVFPALLGRR190 pKa = 11.84LRR192 pKa = 11.84QYY194 pKa = 11.49ALWRR198 pKa = 11.84EE199 pKa = 4.04RR200 pKa = 11.84DD201 pKa = 3.71DD202 pKa = 3.9TLLGALRR209 pKa = 11.84TRR211 pKa = 11.84AVDD214 pKa = 3.1WCRR217 pKa = 11.84AVALRR222 pKa = 11.84PHH224 pKa = 5.85VADD227 pKa = 4.59LAVASCVSIAFEE239 pKa = 4.15PSLHH243 pKa = 6.02EE244 pKa = 4.16RR245 pKa = 11.84STRR248 pKa = 11.84PRR250 pKa = 11.84VDD252 pKa = 2.64RR253 pKa = 11.84HH254 pKa = 4.9MLRR257 pKa = 11.84SPGRR261 pKa = 11.84LSIYY265 pKa = 10.73

Molecular weight: 29.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

759

265

494

379.5

41.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.486 ± 0.383

1.581 ± 0.167

4.743 ± 0.5

5.665 ± 1.03

4.216 ± 0.035

9.091 ± 0.019

1.845 ± 0.183

2.635 ± 0.415

2.503 ± 0.541

10.145 ± 0.435

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.12

1.713 ± 0.316

6.588 ± 0.112

2.767 ± 0.89

10.277 ± 1.185

7.246 ± 0.042

3.557 ± 0.529

9.618 ± 0.511

2.24 ± 0.192

1.976 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski