Propionibacterium phage PA6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pahexavirus; Propionibacterium virus PA6

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4K489|A4K489_9CAUD Gp22 OS=Propionibacterium phage PA6 OX=376758 PE=4 SV=1
MM1 pKa = 7.75GKK3 pKa = 9.4QFWLGLLEE11 pKa = 4.33RR12 pKa = 11.84AAKK15 pKa = 9.74TFVQTFVAVLGVTAGVTYY33 pKa = 9.17TAEE36 pKa = 4.35SFRR39 pKa = 11.84GLPWEE44 pKa = 4.34SALITATVAAVLSVATSFGSPSFVAGKK71 pKa = 9.65PKK73 pKa = 8.27TTPVDD78 pKa = 3.55AGLVPPDD85 pKa = 4.11DD86 pKa = 4.58PGIVEE91 pKa = 3.86PHH93 pKa = 5.87MVDD96 pKa = 3.04VSDD99 pKa = 4.46PGMIEE104 pKa = 3.87PADD107 pKa = 4.07DD108 pKa = 3.31VDD110 pKa = 4.94LGVGYY115 pKa = 10.03VPKK118 pKa = 10.25HH119 pKa = 5.1AAEE122 pKa = 4.43SEE124 pKa = 4.3VGTVEE129 pKa = 4.12STVAA133 pKa = 3.37

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4K4B2|A4K4B2_9CAUD Gp45 OS=Propionibacterium phage PA6 OX=376758 PE=4 SV=1
MM1 pKa = 7.29AHH3 pKa = 6.8SSPHH7 pKa = 6.52PSHH10 pKa = 7.46PSTGRR15 pKa = 11.84QTSTRR20 pKa = 11.84SASTASPEE28 pKa = 4.42TIQTTSIWIWCAACTTLKK46 pKa = 10.58YY47 pKa = 10.19KK48 pKa = 10.59KK49 pKa = 9.39PYY51 pKa = 9.89DD52 pKa = 3.61SSGPRR57 pKa = 11.84GLSPSTAPYY66 pKa = 8.75TATTPGSHH74 pKa = 6.6SLRR77 pKa = 11.84TRR79 pKa = 11.84PRR81 pKa = 11.84NSTISSTT88 pKa = 3.47

Molecular weight: 9.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

8963

51

921

186.7

20.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.852 ± 0.662

1.417 ± 0.214

6.226 ± 0.457

4.697 ± 0.404

2.778 ± 0.247

8.636 ± 0.692

2.7 ± 0.382

4.686 ± 0.369

4.005 ± 0.243

7.364 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.302

3.581 ± 0.262

5.367 ± 0.372

3.972 ± 0.277

6.326 ± 0.348

6.895 ± 0.322

6.594 ± 0.622

7.587 ± 0.803

2.019 ± 0.227

2.722 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski