Mycoplasma canis UFG4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis; Mycoplasmopsis canis

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1A5U9|I1A5U9_9MOLU Type I restriction enzyme S protein OS=Mycoplasma canis UFG4 OX=1131455 GN=MCANUFG4_02291 PE=4 SV=1
MM1 pKa = 6.98IRR3 pKa = 11.84SWKK6 pKa = 9.79EE7 pKa = 3.67SNNVPIKK14 pKa = 11.08GEE16 pKa = 4.5LIDD19 pKa = 5.34AMVFDD24 pKa = 4.98FFEE27 pKa = 4.38NYY29 pKa = 9.62KK30 pKa = 10.37GLKK33 pKa = 9.67SEE35 pKa = 4.6PFNCFDD41 pKa = 4.62EE42 pKa = 4.42YY43 pKa = 11.25TKK45 pKa = 11.31DD46 pKa = 3.31FFEE49 pKa = 4.81YY50 pKa = 9.65IYY52 pKa = 11.06TNIDD56 pKa = 3.64YY57 pKa = 10.64LSQAAIEE64 pKa = 4.07YY65 pKa = 10.5LKK67 pKa = 11.13

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1A4Q1|I1A4Q1_9MOLU 50S ribosomal protein L6 OS=Mycoplasma canis UFG4 OX=1131455 GN=rplF PE=3 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.93KK9 pKa = 10.2AKK11 pKa = 10.03RR12 pKa = 11.84HH13 pKa = 5.81PKK15 pKa = 10.03FSTRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CEE25 pKa = 3.78LCGRR29 pKa = 11.84PHH31 pKa = 6.83SVLRR35 pKa = 11.84KK36 pKa = 9.13YY37 pKa = 10.01KK38 pKa = 9.42VCRR41 pKa = 11.84ICFRR45 pKa = 11.84NLAHH49 pKa = 6.89EE50 pKa = 4.48GKK52 pKa = 10.19IPGVKK57 pKa = 9.38KK58 pKa = 10.89ASWW61 pKa = 3.03

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

642

0

642

262833

31

6955

409.4

46.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.804 ± 0.126

0.294 ± 0.021

5.598 ± 0.075

7.495 ± 0.11

5.549 ± 0.117

4.585 ± 0.112

1.329 ± 0.036

9.439 ± 0.12

10.326 ± 0.128

9.203 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.578 ± 0.057

8.62 ± 0.242

2.556 ± 0.041

2.843 ± 0.053

3.039 ± 0.07

7.49 ± 0.093

5.002 ± 0.103

5.469 ± 0.082

0.869 ± 0.028

3.914 ± 0.08
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski