Conexibacter woesei (strain DSM 14684 / CIP 108061 / JCM 11494 / NBRC 100937 / ID131577)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Thermoleophilia; Solirubrobacterales; Conexibacteraceae; Conexibacter; Conexibacter woesei

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5912 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|D3F094|D3F094_CONWI Major facilitator superfamily MFS_1 OS=Conexibacter woesei (strain DSM 14684 / CIP 108061 / JCM 11494 / NBRC 100937 / ID131577) OX=469383 GN=Cwoe_3536 PE=4 SV=1
MM1 pKa = 7.57SATQTSQKK9 pKa = 9.16WICEE13 pKa = 4.24SCGFIYY19 pKa = 10.56DD20 pKa = 4.59PEE22 pKa = 6.8DD23 pKa = 4.11GDD25 pKa = 4.31PDD27 pKa = 3.95GGIPPGTAFEE37 pKa = 5.84DD38 pKa = 3.83IPEE41 pKa = 4.12TWFCPVCGARR51 pKa = 11.84KK52 pKa = 9.28RR53 pKa = 11.84DD54 pKa = 3.61FVPYY58 pKa = 10.36EE59 pKa = 3.91DD60 pKa = 3.89

Molecular weight:
6.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|D3F9J2|D3F9J2_CONWI Usp domain-containing protein OS=Conexibacter woesei (strain DSM 14684 / CIP 108061 / JCM 11494 / NBRC 100937 / ID131577) OX=469383 GN=Cwoe_0726 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84ASIAAKK8 pKa = 10.07KK9 pKa = 8.81RR10 pKa = 11.84TASAMAIKK18 pKa = 10.44SMPSLWGRR26 pKa = 11.84PRR28 pKa = 11.84PGRR31 pKa = 11.84IKK33 pKa = 10.45IQTAGIKK40 pKa = 9.28MLL42 pKa = 4.23

Molecular weight:
4.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5912

0

5912

1985113

31

4255

335.8

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.9 ± 0.06

0.779 ± 0.008

6.001 ± 0.026

5.739 ± 0.038

2.829 ± 0.016

9.582 ± 0.039

1.966 ± 0.015

3.644 ± 0.023

1.462 ± 0.018

10.486 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.617 ± 0.012

1.676 ± 0.019

5.776 ± 0.03

2.651 ± 0.016

8.727 ± 0.033

4.855 ± 0.026

5.587 ± 0.036

8.594 ± 0.027

1.32 ± 0.014

1.811 ± 0.014

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski