Mycoplasma bovoculi M165/69

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma bovoculi

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 579 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5USN2|W5USN2_9MOLU Guanylate kinase OS=Mycoplasma bovoculi M165/69 OX=743966 GN=gmk PE=3 SV=1
MM1 pKa = 7.66GKK3 pKa = 9.82NKK5 pKa = 9.71ILKK8 pKa = 9.34FYY10 pKa = 10.83SSEE13 pKa = 3.78LCYY16 pKa = 10.7EE17 pKa = 4.05NNGQRR22 pKa = 11.84VCQHH26 pKa = 5.52SLRR29 pKa = 11.84YY30 pKa = 9.47DD31 pKa = 3.19KK32 pKa = 10.3QTIGVFANFFDD43 pKa = 4.81LLVKK47 pKa = 10.42INSLNLVARR56 pKa = 11.84LWVRR60 pKa = 11.84QNKK63 pKa = 9.18KK64 pKa = 9.27FSLVVRR70 pKa = 11.84FPFKK74 pKa = 10.65RR75 pKa = 11.84SLDD78 pKa = 3.82SLNTADD84 pKa = 5.13IQMLLDD90 pKa = 4.9KK91 pKa = 10.71IADD94 pKa = 3.85PSSTGDD100 pKa = 3.48IIEE103 pKa = 3.99QLQYY107 pKa = 10.66TEE109 pKa = 4.49EE110 pKa = 4.27EE111 pKa = 4.3FVQVLEE117 pKa = 4.24EE118 pKa = 4.0QNNNKK123 pKa = 9.66DD124 pKa = 3.74LNISKK129 pKa = 8.94TINKK133 pKa = 9.3ILFDD137 pKa = 4.1VEE139 pKa = 4.99SDD141 pKa = 3.93DD142 pKa = 5.13EE143 pKa = 4.21PVEE146 pKa = 4.86CEE148 pKa = 3.5ICQEE152 pKa = 4.15TLRR155 pKa = 11.84EE156 pKa = 4.05DD157 pKa = 4.99EE158 pKa = 4.75IDD160 pKa = 4.01CGCDD164 pKa = 2.76SIEE167 pKa = 3.64IHH169 pKa = 6.51RR170 pKa = 11.84ADD172 pKa = 4.75FEE174 pKa = 4.88GFSCDD179 pKa = 5.02CPICLSDD186 pKa = 3.64KK187 pKa = 10.42HH188 pKa = 6.25AHH190 pKa = 5.71TYY192 pKa = 9.55EE193 pKa = 3.99EE194 pKa = 4.36PRR196 pKa = 11.84VEE198 pKa = 4.45EE199 pKa = 4.14EE200 pKa = 3.74PLYY203 pKa = 11.03LCEE206 pKa = 4.34CAKK209 pKa = 10.82CLGQEE214 pKa = 3.55VDD216 pKa = 3.56EE217 pKa = 4.53PLNIIEE223 pKa = 4.45EE224 pKa = 4.38TDD226 pKa = 3.99CKK228 pKa = 10.97CEE230 pKa = 4.07SCVKK234 pKa = 10.51GFDD237 pKa = 4.16DD238 pKa = 4.74IKK240 pKa = 10.81PIIIDD245 pKa = 3.6EE246 pKa = 4.56PTTQDD251 pKa = 4.29YY252 pKa = 8.65PCHH255 pKa = 6.0CQNNQQSNAQLIQTYY270 pKa = 9.55FDD272 pKa = 3.52EE273 pKa = 5.21SLYY276 pKa = 11.23SCNCVDD282 pKa = 4.61CASEE286 pKa = 4.01QKK288 pKa = 10.2QVEE291 pKa = 4.42EE292 pKa = 4.71FVDD295 pKa = 4.04EE296 pKa = 4.41PEE298 pKa = 4.35IKK300 pKa = 10.69VGTLLSNSCKK310 pKa = 10.27GVRR313 pKa = 11.84CPISKK318 pKa = 10.52EE319 pKa = 4.04EE320 pKa = 4.15TSDD323 pKa = 3.57EE324 pKa = 4.33PKK326 pKa = 10.49TEE328 pKa = 3.97VLEE331 pKa = 4.09PTIDD335 pKa = 3.66EE336 pKa = 4.49SKK338 pKa = 9.47TAYY341 pKa = 8.85EE342 pKa = 4.06LPYY345 pKa = 10.59EE346 pKa = 4.59CFCHH350 pKa = 6.67EE351 pKa = 4.55CQNPVPSNLDD361 pKa = 3.03YY362 pKa = 10.96FLWFKK367 pKa = 10.73QSQPKK372 pKa = 9.76QIDD375 pKa = 3.24YY376 pKa = 8.38TLPYY380 pKa = 9.96EE381 pKa = 4.63CQCHH385 pKa = 5.72LCSGQEE391 pKa = 3.79IEE393 pKa = 5.83LSLDD397 pKa = 3.5YY398 pKa = 10.84ILAEE402 pKa = 3.73TSVNKK407 pKa = 9.92EE408 pKa = 3.58QLEE411 pKa = 4.26EE412 pKa = 3.92VLIADD417 pKa = 4.12EE418 pKa = 5.3PIIVDD423 pKa = 3.44NFEE426 pKa = 4.4SKK428 pKa = 10.9SEE430 pKa = 3.88ILCNCDD436 pKa = 4.08HH437 pKa = 6.81NEE439 pKa = 3.92CDD441 pKa = 4.57EE442 pKa = 4.52PQLDD446 pKa = 4.22PIVNLDD452 pKa = 3.91HH453 pKa = 7.35LNAPLLAPTTTATNFEE469 pKa = 4.73CPACQHH475 pKa = 5.94VATCDD480 pKa = 3.26FCQKK484 pKa = 10.51YY485 pKa = 10.19KK486 pKa = 11.02LYY488 pKa = 10.39LLEE491 pKa = 6.12AIEE494 pKa = 5.5CPGCQKK500 pKa = 10.71LFADD504 pKa = 4.28RR505 pKa = 11.84GISIEE510 pKa = 4.32EE511 pKa = 3.92LLKK514 pKa = 10.95SYY516 pKa = 10.13FPHH519 pKa = 7.22ISYY522 pKa = 10.41DD523 pKa = 3.82GKK525 pKa = 10.71SHH527 pKa = 7.0GSQLCSLSTSEE538 pKa = 5.08QEE540 pKa = 4.72CPLAKK545 pKa = 10.6LKK547 pKa = 10.33DD548 pKa = 3.51QKK550 pKa = 11.11FIDD553 pKa = 3.63LHH555 pKa = 6.9DD556 pKa = 4.22EE557 pKa = 3.91NRR559 pKa = 11.84GIIYY563 pKa = 9.3TPEE566 pKa = 5.76DD567 pKa = 3.83GVSSGDD573 pKa = 3.47YY574 pKa = 11.11DD575 pKa = 3.68EE576 pKa = 6.01LLEE579 pKa = 4.83GNVNTKK585 pKa = 10.02RR586 pKa = 11.84GYY588 pKa = 10.36GGGGVGGPATGQGVGRR604 pKa = 11.84FAVDD608 pKa = 3.25DD609 pKa = 3.9TSAIIGAVSEE619 pKa = 4.25KK620 pKa = 10.67NKK622 pKa = 9.91PDD624 pKa = 3.5EE625 pKa = 4.78EE626 pKa = 4.39IVLVTTKK633 pKa = 9.54KK634 pKa = 8.88TGFWGIPAYY643 pKa = 9.81VLWFIVFALIAAIIIIVVMFFVFGSGLII671 pKa = 3.78

Molecular weight: 75.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5V0R2|W5V0R2_9MOLU Putative phase variable surface protein VpbC OS=Mycoplasma bovoculi M165/69 OX=743966 GN=vpbC PE=4 SV=1
MM1 pKa = 7.49ALKK4 pKa = 10.12HH5 pKa = 5.7YY6 pKa = 10.68KK7 pKa = 9.88PVTNGRR13 pKa = 11.84RR14 pKa = 11.84HH15 pKa = 5.13MSALNYY21 pKa = 10.01RR22 pKa = 11.84EE23 pKa = 4.25NLTTDD28 pKa = 3.35RR29 pKa = 11.84PEE31 pKa = 4.01KK32 pKa = 10.57SLVVILKK39 pKa = 9.92KK40 pKa = 10.26HH41 pKa = 5.57SGRR44 pKa = 11.84NNQGKK49 pKa = 8.05ITTRR53 pKa = 11.84HH54 pKa = 4.31QGGRR58 pKa = 11.84QKK60 pKa = 10.67RR61 pKa = 11.84KK62 pKa = 9.61YY63 pKa = 10.33RR64 pKa = 11.84LIDD67 pKa = 3.67FKK69 pKa = 11.14RR70 pKa = 11.84NKK72 pKa = 10.2DD73 pKa = 3.69NIVAIVKK80 pKa = 9.09TIEE83 pKa = 3.71YY84 pKa = 10.37DD85 pKa = 3.49PNRR88 pKa = 11.84SANIALVSYY97 pKa = 10.98KK98 pKa = 10.57DD99 pKa = 3.44GEE101 pKa = 4.16KK102 pKa = 10.29RR103 pKa = 11.84YY104 pKa = 9.94ILAPKK109 pKa = 9.29GLQVGQQVISGDD121 pKa = 3.58NVDD124 pKa = 3.69ILVGNTLPLANIPEE138 pKa = 4.48GTFVHH143 pKa = 6.69NIEE146 pKa = 4.36LHH148 pKa = 6.19PGAGGQLMRR157 pKa = 11.84SAGASAQIQGRR168 pKa = 11.84DD169 pKa = 3.17EE170 pKa = 4.47DD171 pKa = 3.91GKK173 pKa = 11.17YY174 pKa = 10.87VILKK178 pKa = 9.66LKK180 pKa = 9.58SGEE183 pKa = 3.79YY184 pKa = 9.85RR185 pKa = 11.84RR186 pKa = 11.84ILARR190 pKa = 11.84CRR192 pKa = 11.84ATIGVVGNEE201 pKa = 3.79EE202 pKa = 3.93HH203 pKa = 6.97SLVNIGKK210 pKa = 9.86AGRR213 pKa = 11.84SRR215 pKa = 11.84HH216 pKa = 5.5LGIRR220 pKa = 11.84PTVRR224 pKa = 11.84GSVMNPNDD232 pKa = 3.69HH233 pKa = 6.5PHH235 pKa = 6.6GGGEE239 pKa = 4.24GKK241 pKa = 10.04QPIGRR246 pKa = 11.84KK247 pKa = 9.29SPLTPWGKK255 pKa = 10.11KK256 pKa = 9.7ALGVKK261 pKa = 7.53TRR263 pKa = 11.84RR264 pKa = 11.84NKK266 pKa = 10.08KK267 pKa = 10.26ASTKK271 pKa = 10.47LIIRR275 pKa = 11.84RR276 pKa = 11.84RR277 pKa = 11.84KK278 pKa = 9.64DD279 pKa = 3.11SKK281 pKa = 11.34

Molecular weight: 31.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

579

0

579

230652

37

3779

398.4

45.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.559 ± 0.115

0.407 ± 0.028

5.424 ± 0.091

6.049 ± 0.114

6.154 ± 0.09

4.938 ± 0.098

1.411 ± 0.035

9.072 ± 0.127

9.777 ± 0.11

9.298 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.37 ± 0.042

7.514 ± 0.121

2.992 ± 0.055

4.55 ± 0.067

2.643 ± 0.06

7.38 ± 0.08

5.367 ± 0.125

5.554 ± 0.074

0.928 ± 0.03

3.615 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski